Abstract:
BACKGROUND:Adipose-derived stem cells and other stromal vascular fraction cells were used more often for stem cell therapy, even though limitations such as poor cell retention rate, complicated and expensive isolation processes, and the use of specific laboratory equipment need to be overcome. METHODS:Here, the authors developed a novel but simple method for generating an injectable mixture of stromal vascular fraction cells and native adipose extracellular matrix. It is a purely mechanical process in which lipoaspirate is processed into an extracellular matrix/stromal vascular fraction gel. The standard processing procedure was established using quantized tests. The therapeutic potential of the product for wound healing was then tested. RESULTS:Extracellular matrix/stromal vascular fraction gel derived from lipoaspirate and processed using a standard Coleman technique, followed by 1 minute of mechanical processing by passage back and forth between two 10-ml syringes at a flow rate of 10 ml/second, showed the highest adipose-derived stem cell and endothelial cell density. The stromal vascular fraction cells within the product also showed potential for multipotent differentiation similar to that of normal fat samples. In addition, the product showed better therapeutic results than stromal vascular fraction cell suspension when used to treat a nude mouse model of wound healing. CONCLUSIONS:Extracellular matrix/stromal vascular fraction gel is an autologous injectable derived from native extracellular matrix and is a functional cellular component generated using a simple mechanical process. As such, it may offer a novel mode of tissue repair suitable for clinical application in stem cell therapies.
journal_name
Plast Reconstr Surgjournal_title
Plastic and reconstructive surgeryauthors
Yao Y,Dong Z,Liao Y,Zhang P,Ma J,Gao J,Lu Fdoi
10.1097/PRS.0000000000003214subject
Has Abstractpub_date
2017-04-01 00:00:00pages
867-879issue
4eissn
0032-1052issn
1529-4242pii
00006534-201704000-00019journal_volume
139pub_type
杂志文章abstract:BACKGROUND:Surgical-site infection is a common postoperative nosocomial infection. Surgeons frequently treat operative patients with protective antibiotics and often choose cefazolin as the drug. Treatment schemes include both preoperative intravenous dosing and intraoperative dosing by irrigation. This study was desig...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,随机对照试验
doi:10.1097/PRS.0b013e31818d5899
更新日期:2008-12-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to assess the efficacy of botulinum toxin in reducing the frequency of migraine headaches. METHODS:The MEDLINE, Embase, and Cochrane Library databases were searched to identify randomized, double-blind, placebo-controlled trials that compared patients receiving botulinum toxin ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,meta分析
doi:10.1097/PRS.0000000000005111
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Modern breast reconstruction often involves microvascular reconstruction. The most common recipient vessels are the internal mammary artery and vein. Recently, there has been great focus on efficiency, but much of this emphasis has been directed to faster flap harvest or recovery protocols for expeditious di...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000003774
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:To plan abdominal perforator-based microsurgical breast reconstruction, duplex ultrasound is often employed to preoperatively identify the location of abdominal wall perforating vessels. Recently, several groups have published the use of computed tomography angiography for preoperative planning in perforator...
journal_title:Plastic and reconstructive surgery
pub_type: 临床试验,杂志文章
doi:10.1097/PRS.0b013e3181c82d24
更新日期:2010-02-01 00:00:00
abstract::Although endothelial cell injury and microcirculatory intravascular thrombosis have been implicated in the pathophysiology of skin-flap failure, the basic underlying pathophysiology has not been documented previously. This study focuses on the morphologic changes and the alteration in platelet, fibrinogen, antithrombi...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199402000-00024
更新日期:1994-02-01 00:00:00
abstract::An experimental, anatomical, and clinical study has confirmed the feasibility of free neurovascular flaps. The interrelationship between the cutaneous vascular and neural supply has been defined and demonstrated electrophysiologically, both in man and in experimental animals. A potential donor site in man has been e...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-197507000-00004
更新日期:1975-07-01 00:00:00
abstract:BACKGROUND:Our understanding of breast shape and size changes after surgery is limited by a lack of measurement studies that evaluate and compare changes in breast dimensions after cosmetic breast procedures. This study was undertaken to remedy this deficiency. METHODS:Women undergoing primary vertical mastopexy, augm...
journal_title:Plastic and reconstructive surgery
pub_type: 临床试验,杂志文章
doi:10.1097/PRS.0b013e3182865e20
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:High-energy trauma or peripheral arteriopathy may render recipient vessels unsuitable for free tissue transfer. Vein grafts can be used to lengthen the vascular pedicles to reach healthy distant vessels but with an increased complication rate. METHODS:From 1995 to 2006, 56 arteriovenous loops were construct...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/01.prs.0000297634.53915.e5
更新日期:2008-02-01 00:00:00
abstract::After reviewing the historical aspects of the various lip-switch flaps, the authors draw attention to the existence of a report of a lip-switch flap performed in Sweden in 1756 by J.G.Hierzel. ...
journal_title:Plastic and reconstructive surgery
pub_type: 传,历史文章,杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Hospital readmissions have become a topic of focus for quality care measures and cost-reduction efforts. However, no comparative multi-institutional data on plastic surgery outpatient readmission rates currently exist. The authors endeavored to investigate hospital readmission rates and predictors of readmis...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/01.prs.0000436833.11442.8d
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:This study was proposed to evaluate the facial contour and electrophysiologic changes of the masseter and temporalis muscles before and after botulinum toxin A injection in the wide lower face (square face). METHODS:The botulinum toxin A injections were performed on 10 patients for the treatment of square f...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/01.prs.0000271068.71399.ae
更新日期:2007-09-01 00:00:00
abstract::A technique is presented for correction of supratarsal depression in the upper lids of orientals, using silicone bag-gel prostheses. ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-197812000-00005
更新日期:1978-12-01 00:00:00
abstract:BACKGROUND:Peripheral neuropathy is highly prevalent among patients with diabetes mellitus and demonstrates well-established consequences of limb loss following lower extremity ulceration, infection, and amputation and upper extremity loss of function. Symptomatic neuropathy is recognized by neuropathic pain, paresthes...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,评审
doi:10.1097/PRS.0b013e31820063d4
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Breast reconstruction after breast cancer surgery has come to be regarded as an achievable treatment option to improve patients' quality of life. Although it has been pointed out that surgeons' information-giving behaviors affect women's decision to undergo breast reconstruction surgery, little is known abou...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/01.prs.0000233149.36751.d2
更新日期:2006-12-01 00:00:00
abstract::The adenosine triphosphate (ATP) content of keloids and scars resected from patients was demonstrated by high-performance liquid chromatography. The surface color of hypertrophic scars was red or pink and that of atrophic scars was white. The ATP content of red scars was (in mmol/g protein) 1.06 +/- 0.14, of pink scar...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199909030-00012
更新日期:1999-09-01 00:00:00
abstract::Ehlers-Danlos syndrome is an inherited collagen disorder characterized by skin hyperextensibility, joint laxity, and tissue friability. In this study, it was hypothesized that Ehlers-Danlos syndrome is frequently undiagnosed in patients who present for repair of ventral abdominal wall hernias. A retrospective chart re...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-200012000-00012
更新日期:2000-12-01 00:00:00
abstract::The anatomy of the vascular connections between the eighth intercostal vessels and the deep epigastric vessels was studied in a series of 20 dissections in 10 preserved cadavers to develop the rectus abdominis myocutaneous flap combined with a vascularized rib. The distance between the point where the eighth intercost...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199511000-00015
更新日期:1995-11-01 00:00:00
abstract::The case series is the most prevalent type of clinical research in the plastic surgery literature. However, this is a lower level study design in the hierarchy of evidence. The case series is nevertheless a useful hypothesis generator for future studies. These in turn can be tested with more robust study designs such ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,评审
doi:10.1097/PRS.0b013e318221f2ec
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Diced cartilage grafts are useful for dorsal nasal augmentation; however, long-term resorption usually occurs. The aim of this study was to examine the effects of adipose-derived stem cells on the viability of diced cartilage grafts. METHODS:Twenty Fischer rats were divided into four groups with diced carti...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0b013e31822b65fd
更新日期:2012-02-01 00:00:00
abstract::The purpose of this study was to gain information which might be helpful in understanding the influence of palate surgery on abnormal maxillary growth. The finding of actin-rich cells in the granulation tissue of palatal wounds supports the hypothesis that one of the functions of those cells is contraction--and the la...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-197809000-00015
更新日期:1978-09-01 00:00:00
abstract:BACKGROUND:Wound complications after perineal and groin obliterative procedures are a significant cause of morbidity, particularly following chemoradiation therapy. Vertical and, increasingly, oblique rectus abdominis myocutaneous flaps have been used to fill potential dead space and bring healthy, vascularized tissue ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000000324
更新日期:2014-08-01 00:00:00
abstract:LEARNING OBJECTIVES:After studying this article, the participant should be able to: 1. Identify clinical situations in which hand sonography can result in the detection of partial extensor tendon tears. 2. Identify the limitations of magnetic resonance imaging in diagnosing extensor tendon tears. 3. Understand the vari...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,评审
doi:10.1097/01.prs.0000345599.95343.2a
更新日期:2009-02-01 00:00:00
abstract::Cultured epidermal skin has become an adjunctive therapy for treatment of major burn injuries, but its effectiveness is greatly limited due to destruction by microbial contamination. To evaluate candidate drugs for use with cultured skin, a combined cytotoxicity-antimicrobial assay system was developed for determinati...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199309000-00018
更新日期:1993-09-01 00:00:00
abstract::Twenty-six nail deformities secondary to ganglions of the distal interphalangeal joint were retrospectively reviewed to assess the important aspects of their management. The patients' ages ranged from 41 to 79 years. The long and index fingers were most commonly involved. A depression or groove was present in 23 of 26...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-199104000-00017
更新日期:1991-04-01 00:00:00
abstract:BACKGROUND:Acellular dermal matrix is a commonly used adjunct in implant-based breast reconstruction. Several investigations have shown increased complications associated with its use. Therefore, the authors' institution placed strict limitations on its use and transitioned to sterile "ready-to-use" acellular dermal ma...
journal_title:Plastic and reconstructive surgery
pub_type: 临床试验,杂志文章
doi:10.1097/PRS.0b013e31829fe35b
更新日期:2013-10-01 00:00:00
abstract::We report a case in which an undelayed cervical myocutaneous flap was used early to cover the denuded cartilage of a burned ear. The ear was saved, and a good result was obtained. ...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/00006534-197810000-00030
更新日期:1978-10-01 00:00:00
abstract:BACKGROUND:The combination of sedative and analgesic drugs is increasingly being used during minimally invasive surgery. The authors compared the clinical efficacy of two different fentanyl regimens, in combination with midazolam, for sedation analgesia in patients undergoing office-based plastic surgery procedures und...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,随机对照试验
doi:10.1097/01.prs.0000260754.59310.38
更新日期:2007-06-01 00:00:00
abstract:LEARNING OBJECTIVES:After studying this article, the participant should be able to: 1. Select appropriate local and pedicled flaps for oral cavity reconstruction based on the location and extent of the defect and donor-site characteristics. 2. Understand the functions of the tongue and select the appropriate reconstruc...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,评审
doi:10.1097/PRS.0000000000000722
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:A previous report indicated that secondary rhinoplasty patients with normal preoperative noses displayed significantly higher prevalences of depression, demanding behavior, previous aesthetic operations, and confirmed trauma (abuse/neglect) histories than patients who originally had dorsal deformities or str...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章
doi:10.1097/PRS.0000000000000552
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Carpal tunnel syndrome is very common. Although surgery is effective in mild and moderate cases, recovery is often incomplete in severe cases. Therefore, adjuvant therapy to improve nerve regeneration in those patients is much needed. Acetyl-L-carnitine has been shown to be effective in other neuropathies. T...
journal_title:Plastic and reconstructive surgery
pub_type: 杂志文章,随机对照试验
doi:10.1097/PRS.0000000000005089
更新日期:2019-01-01 00:00:00