Selection of topical antimicrobial agents for cultured skin for burns by combined assessment of cellular cytotoxicity and antimicrobial activity.


:Cultured epidermal skin has become an adjunctive therapy for treatment of major burn injuries, but its effectiveness is greatly limited due to destruction by microbial contamination. To evaluate candidate drugs for use with cultured skin, a combined cytotoxicity-antimicrobial assay system was developed for determination of toxicity to cultured human keratinocytes and fibroblasts, and to common burn wound organisms (20 bacterial and 4 fungal strains). Candidate agents including Hibiclens (n = 3), amikacin, piperacillin, norfloxacin, and nystatin were tested separately and in combination (n = 6 each) for inhibition of growth of human cells and lytic activity on microorganisms in the wet disc assay. The data showed that: (1) Hibiclens was uniformly toxic to both cultured human cells and microorganisms; (2) norfloxacin had dose-dependent toxicity to human cells and broad effectiveness against microorganisms; and (3) norfloxacin (25 micrograms/mL) plus nystatin (100 U/mL) had low toxicity to human cells and high toxicity to both Gram-positive and Gram-negative bacteria (20 of 20) and fungi (4 of 4). Selection of topical antimicrobial drugs by these assays may improve effectiveness of cultured skin for burns and may be extended to the control of other surgical wound infections.


Plast Reconstr Surg


Boyce ST,Holder IA




Has Abstract


1993-09-01 00:00:00












  • The intravascular stenting method for treatment of extremity lymphedema with multiconfiguration lymphaticovenous anastomoses.

    abstract:BACKGROUND:In secondary extremity lymphedema, normal antegrade lymphatic flow is disrupted by the disease state. Attempts to capture aberrant retrograde lymphatic flow by means of microsurgical lymphaticovenous anastomoses have been hindered because of technical limitations. The authors applied the intravascular stenti...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Narushima M,Mihara M,Yamamoto Y,Iida T,Koshima I,Mundinger GS

    更新日期:2010-03-01 00:00:00

  • Delayed-immediate breast reconstruction: technical and timing considerations.

    abstract:BACKGROUND:In 2002, the author implemented a new two-stage approach, "delayed-immediate breast reconstruction," for patients who desire breast reconstruction and who are at an increased risk for conditions necessitating postmastectomy radiotherapy. There is increasing acceptance of this approach in clinical practice. T...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Kronowitz SJ

    更新日期:2010-02-01 00:00:00

  • Does the pulsed tunable dye laser have a role in the management of infantile hemangiomas? Observations based on 3 years' experience.

    abstract::Over a 3-year period, 50 patients with strawberry hemangiomas and 7 patients with postinvolutional redness or telangiectasia were treated with the SPTL-1 laser (Candela Corporation, Wayland, Mass.). The SPTL-1 laser emits pulsed light at a wavelength of 585 nm with a pulse duration of 450 ms. Lesions with a superficia...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Scheepers JH,Quaba AA

    更新日期:1995-02-01 00:00:00

  • Neurovascular free flaps. A preliminary report.

    abstract::An experimental, anatomical, and clinical study has confirmed the feasibility of free neurovascular flaps. The interrelationship between the cutaneous vascular and neural supply has been defined and demonstrated electrophysiologically, both in man and in experimental animals. A potential donor site in man has been e...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Daniel RK,Terzis J,Schwarz G

    更新日期:1975-07-01 00:00:00

  • Increasing the volume of vascularized tissue formation in engineered constructs: an experimental study in rats.

    abstract::The authors have previously described a model of in vivo tissue generation based on an implanted, microsurgically created vessel loop in a plastic chamber (volume, 0.45 ml) containing a poly(DL-lactic-co-glycolic acid) (PLGA) scaffold. Tissue grew spontaneously in association with an intense angiogenic sprouting from ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Hofer SO,Knight KM,Cooper-White JJ,O'Connor AJ,Perera JM,Romeo-Meeuw R,Penington AJ,Knight KR,Morrison WA,Messina A

    更新日期:2003-03-01 00:00:00

  • Subperiosteal rejuvenation of the midface and periorbital area: a simplified approach.

    abstract::A simplified approach to subperiosteal midface rejuvenation is presented. The technique was developed through experience with 60 subperiosteal face lifts over an 8-year period. Because it does not involve a lower eyelid incision, there is no risk of ectropion, yet the midfacial structures and orbicularis oculi muscle ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Hobar PC,Flood J

    更新日期:1999-09-01 00:00:00

  • Quantitative assessment of osseous, ocular, and periocular changes after hypertelorism surgery.

    abstract::The purpose of this study was to develop a methodology to quantify osseous, ocular, and periocular fat changes caused by correction of orbital hypertelorism to test the hypothesis that there is a quantitatively predictable relationship between the movement of the osseous orbit and that of the ocular globe. A retrospec...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Panchal J,Kim YO,Stelnicki E,Pilgram T,Marsh JL

    更新日期:1999-07-01 00:00:00

  • Scalp stretching with a tissue expander for closure of scalp defects.

    abstract::The authors show a way of reconstruction of scalp defects with excellent results using a tissue expander. This method creates "new" scalp tissue for coverage of defects with normal hair-bearing skin having a dense and even growth of hair. The hairs grow in the correct direction. The authors also show that almost all o...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Nordström RE,Devine JW

    更新日期:1985-04-01 00:00:00

  • Expression of androgen, estrogen, progesterone, and growth hormone receptors in vascular malformations.

    abstract:BACKGROUND:Vascular malformations frequently enlarge during adolescence, suggesting that hormones may be involved. The purpose of this study was to determine whether pubertal hormone receptors are present in vascular malformations and whether they differ from normal tissue. METHODS:Tissue specimens (arteriovenous malf...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Kulungowski AM,Hassanein AH,Nosé V,Fishman SJ,Mulliken JB,Upton J,Zurakowski D,DiVasta AD,Greene AK

    更新日期:2012-06-01 00:00:00

  • rhBMP-4 gene therapy in a juvenile canine alveolar defect model.

    abstract:BACKGROUND:Autologous bone grafts have the disadvantages of donor-site pain and morbidity, finite supply, increased costs, and prolonged hospitalizations. Using a juvenile canine model, the authors hypothesized that recombinant human (rh) bone morphogenetic protein (BMP)-4 gene therapy-treated alveolar defects would pr...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Chen JC,Winn SR,Gong X,Ozaki WH

    更新日期:2007-11-01 00:00:00

  • External distraction osteogenesis in the pediatric mandible.

    abstract:SUMMARY:Mandibular distraction osteogenesis is an effective tool with which to correct facial asymmetry, restore proper occlusion and, more importantly, create an appropriate airway in the micrognathic infant. As a component of many syndromes, micrognathia can impair feeding and speech development and can obstruct the ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Kaufman Y,Cole PD,McKnight A,Hatef DA,Hollier LH

    更新日期:2009-01-01 00:00:00

  • Craniectomy gap patency and neosuture formation following endoscopic suturectomy for unilateral coronal craniosynostosis.

    abstract:BACKGROUND:The combination of endoscope-assisted suturectomy and postoperative helmet therapy has been advocated to treat unilateral coronal synostosis. However, surgical outcomes can vary. One possible explanation for this inconsistency is early closure of the craniectomy gap. The authors examined short-term postopera...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Sauerhammer TM,Seruya M,Ropper AE,Oh AK,Proctor MR,Rogers GF

    更新日期:2014-07-01 00:00:00

  • Middle Eastern rhinoplasty in the United States: Part II. Secondary rhinoplasty.

    abstract:BACKGROUND:There have been relatively few articles in the English language on secondary Middle Eastern rhinoplasty. This article analyzes the cause and treatment of secondary Middle Eastern rhinoplasty. METHODS:A prospective study of 40 consecutive female secondary Middle Eastern rhinoplasty patients was completed. R...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Daniel RK

    更新日期:2009-11-01 00:00:00

  • Labial incompetence: a marker for progressive bone resorption in silastic chin augmentation.

    abstract::Bone erosion below silicone rubber implants is a wellknown sequela, occurring in a majority of patients undergoing augmentation genioplasty. It is generally considered to be self-limiting and with minimal adverse consequences. However, if bone erosion progresses to the extent to which teeth are jeopardized or alterati...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Matarasso A,Elias AC,Elias RL

    更新日期:1996-11-01 00:00:00

  • One-stage lip-switch operation.

    abstract::A one-stage lip-switch operation was successfully performed on an 80-year-old woman with accompanying disorders. Postoperative venous congestion was controlled mainly by dressing changes with wet gauze. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Ohtsuka H

    更新日期:1985-10-01 00:00:00

  • Determining the Safety and Efficacy of Gluteal Augmentation: A Systematic Review of Outcomes and Complications.

    abstract:BACKGROUND:Augmentation gluteoplasty has been performed more frequently in the past decade, with over 21,000 procedures performed in the past year alone. The most popular methods for buttock augmentation involve silicone prostheses and autologous fat grafting. A comparison of complications of these two techniques does ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审


    authors: Sinno S,Chang JB,Brownstone ND,Saadeh PB,Wall S Jr

    更新日期:2016-04-01 00:00:00

  • The Associations of Hospital Volume, Surgeon Volume, and Surgeon Experience with Complications and 30-Day Rehospitalization after Free Tissue Transfer: A National Population Study.

    abstract:BACKGROUND:Greater provider volume is associated with better outcomes. There is, however, a paucity of evidence on volume-outcome associations for surgical complications and 30-day all-cause rehospitalization after free tissue transfer or free flap surgery. Surgical complications and frequent rehospitalization are impo...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,多中心研究


    authors: Mahmoudi E,Lu Y,Chang SC,Lin CY,Wang YC,Chang CJ,Cheng MH,Chung KC

    更新日期:2017-08-01 00:00:00

  • Nonepidermal origin of malignant melanoma associated with a giant congenital nevocellular nevus.

    abstract::We report an adult who developed primary malignant melanoma arising in association with residual nevus cells in the pectoralis muscle under an intact skin graft 13 years following the partial excision of a giant congenital nevocellular nevus (GCNN). The case serves to emphasize the malignant potential of GCNN, for whi...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Rhodes AR,Wood WC,Sober AJ,Mihm MC Jr

    更新日期:1981-06-01 00:00:00

  • The history of injectable silicone fluids for soft-tissue augmentation.

    abstract:BACKGROUND:The debate over the legitimacy of silicone as a safe tool for soft-tissue augmentation has spanned well over half a century. Proponents concede that injections of questionable purity and/or of massive quantities have produced unfavorable outcomes. They assert that in experienced hands with "injectable-grade"...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审


    authors: Chasan PE

    更新日期:2007-12-01 00:00:00

  • Revascularization of cranial versus iliac crest bone grafts in the rat.

    abstract::Full-thickness cranial (membranous) and split-thickness iliac crest (endochondral) onlay bone grafts were placed subperiosteally without fixation onto the snout (membranous) and tibia (endochondral) in 30 rats. The animals had been divided into three equal groups in which the bone grafts had been demineralized, autocl...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Sullivan WG,Szwajkun PR

    更新日期:1991-06-01 00:00:00

  • Anxiety disorders in plastic surgery.

    abstract::Surgery is a stressful event, with the potential for profound disturbance to the patient's psychological and physiologic homeostasis. Cosmetic surgery is a particularly intense psychological experience because, in addition to the usual concerns about surgical side effects, cosmetic patients bring their hopes and expec...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Rankin M,Borah GL

    更新日期:1997-08-01 00:00:00

  • Mechanisms of murine cranial suture patency mediated by a dominant negative transforming growth factor-beta receptor adenovirus.

    abstract::Using a physiologic model of mouse cranial suture fusion, the authors' laboratory has previously demonstrated that transforming growth factor (TGF)-betas appear to be more abundantly expressed in the suture complex of the fusing posterior frontal compared with the patent sagittal suture. Furthermore, the authors have ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Song HM,Fong KD,Nacamuli RP,Warren SM,Fang TD,Mathy JA,Cowan CM,Aalami OO,Longaker MT

    更新日期:2004-05-01 00:00:00

  • Complications after polyacrylamide hydrogel injection for facial soft-tissue augmentation in China: twenty-four cases and their surgical management.

    abstract:BACKGROUND:Polyacrylamide hydrogel has been used for soft-tissue augmentation for more than 10 years. Although it is considered a nontoxic, nonimmunogenic material, complications after polyacrylamide hydrogel injections during facial soft-tissue augmentation have been reported. METHODS:Between 2003 and 2009, 24 patien...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Shen H,Lv Y,Xu JH,Hong XY,Zeng BW,Xiao W,Zheng LJ

    更新日期:2012-08-01 00:00:00

  • Five-year outcome of surgical treatment of migraine headaches.

    abstract:BACKGROUND:This study was designed to assess the long-term efficacy of surgical deactivation of migraine headache trigger sites. METHODS:One hundred twenty-five volunteers were randomly assigned to the treatment (n = 100) or control group (n = 25) after examination by the team neurologist to ensure a diagnosis of migr...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,随机对照试验


    authors: Guyuron B,Kriegler JS,Davis J,Amini SB

    更新日期:2011-02-01 00:00:00

  • Role of Early Application of Pressure Garments following Burn Injury and Autografting.

    abstract:BACKGROUND:Pressure garment therapy, used for reduction of postburn scarring, is commonly initiated after complete healing of the wound or autograft. Although some clinicians have suggested that earlier treatment may improve outcomes, the effect of early initiation of therapy has not been studied in a controlled enviro...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: DeBruler DM,Baumann ME,Blackstone BN,Zbinden JC,McFarland KL,Bailey JK,Supp DM,Powell HM

    更新日期:2019-02-01 00:00:00

  • Comparison of vascular hemodynamics in experimental models of microvascular anastomoses.

    abstract::Blood flow was measured in the canine saphenous artery using electromagnetic flowmetry. Significant increase in blood flow was noted after occlusion of the distal femoral artery. However, after raising a saphenous island flap there was no significant change in the blood flow before and after distal femoral artery occl...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Rao VK,Morrison WA,Angus JA,O'Brien BM

    更新日期:1983-02-01 00:00:00

  • Use of the vascularized free fibula graft with an arteriovenous loop for fusion of cervical and thoracic spinal defects in previously irradiated pediatric patients.

    abstract:BACKGROUND:Extensive spinal neoplasms are difficult to manage. Following resection, arthrodesis of the spine can be performed with instrumentation, but this often fails in the setting of radiation therapy. Use of the free fibula flap for anterior spinal fusion to correct deformities has been described in multiple studi...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Jandali S,Diluna ML,Storm PB,Low DW

    更新日期:2011-05-01 00:00:00

  • Clinical experience with Heyer-Schulte inflatable implants in breast augmentation.

    abstract::Following breast augmentation, 100 patients with inflatable saline implants were followed for a period of 6 months to 8 1/2 years. Capsular contracture rate, Baker II or greater, was 3.4 percent. This low rate of capsular contracture is attributed to early manipulation of the implant. The deflation rate was also 3.4 p...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Capozzi A

    更新日期:1986-05-01 00:00:00

  • Salvage of proximal humeral amputations with a remnant forearm flap.

    abstract::Spare-part surgery of upper extremity avulsions may facilitate primary soft-tissue healing and prosthetic rehabilitation. This procedure also may be applicable to the management of analogous proximal lower extremity avulsions where a short femoral stump and a shortage of soft tissue exist. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Mixter RC,Rao VK,De Angelis A,Donald CE

    更新日期:1991-05-01 00:00:00

  • The internal oblique muscle flap: an anatomic and clinical study.

    abstract::A new muscle flap based on the ascending branch of the deep circumflex iliac artery is described. Twenty internal oblique muscle flaps have been dissected and studied in 10 fresh cadavers. This muscle flap has been used successfully as a free-tissue transfer in seven lower extremity defects. There was one loss of flap...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Ramasastry SS,Tucker JB,Swartz WM,Hurwitz DJ

    更新日期:1984-05-01 00:00:00