Selection of topical antimicrobial agents for cultured skin for burns by combined assessment of cellular cytotoxicity and antimicrobial activity.

Abstract:

:Cultured epidermal skin has become an adjunctive therapy for treatment of major burn injuries, but its effectiveness is greatly limited due to destruction by microbial contamination. To evaluate candidate drugs for use with cultured skin, a combined cytotoxicity-antimicrobial assay system was developed for determination of toxicity to cultured human keratinocytes and fibroblasts, and to common burn wound organisms (20 bacterial and 4 fungal strains). Candidate agents including Hibiclens (n = 3), amikacin, piperacillin, norfloxacin, and nystatin were tested separately and in combination (n = 6 each) for inhibition of growth of human cells and lytic activity on microorganisms in the wet disc assay. The data showed that: (1) Hibiclens was uniformly toxic to both cultured human cells and microorganisms; (2) norfloxacin had dose-dependent toxicity to human cells and broad effectiveness against microorganisms; and (3) norfloxacin (25 micrograms/mL) plus nystatin (100 U/mL) had low toxicity to human cells and high toxicity to both Gram-positive and Gram-negative bacteria (20 of 20) and fungi (4 of 4). Selection of topical antimicrobial drugs by these assays may improve effectiveness of cultured skin for burns and may be extended to the control of other surgical wound infections.

journal_name

Plast Reconstr Surg

authors

Boyce ST,Holder IA

doi

10.1097/00006534-199309000-00018

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

493-500

issue

3

eissn

0032-1052

issn

1529-4242

journal_volume

92

pub_type

杂志文章
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    doi:

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    doi:

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  • Scalp stretching with a tissue expander for closure of scalp defects.

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  • Determining the Safety and Efficacy of Gluteal Augmentation: A Systematic Review of Outcomes and Complications.

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    pub_type: 杂志文章,多中心研究

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  • The history of injectable silicone fluids for soft-tissue augmentation.

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  • Anxiety disorders in plastic surgery.

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  • Mechanisms of murine cranial suture patency mediated by a dominant negative transforming growth factor-beta receptor adenovirus.

    abstract::Using a physiologic model of mouse cranial suture fusion, the authors' laboratory has previously demonstrated that transforming growth factor (TGF)-betas appear to be more abundantly expressed in the suture complex of the fusing posterior frontal compared with the patent sagittal suture. Furthermore, the authors have ...

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    pub_type: 杂志文章

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  • Complications after polyacrylamide hydrogel injection for facial soft-tissue augmentation in China: twenty-four cases and their surgical management.

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    更新日期:2012-08-01 00:00:00

  • Five-year outcome of surgical treatment of migraine headaches.

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    更新日期:2011-02-01 00:00:00

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