Abstract：
:Hernandezine, a novel anticancer AMPK activator, is a major active constituent of Thalictrum Ranunculaceae. A simple, specific and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantification of hernandezine in rat plasma and tissues after intravenous administration. Sample preparation was carried out through a protein-precipitation extraction with acetonitrile using tetrandrine as internal standard (IS). The chromatographic separation was achieved by using an Agilent ZORBAX Eclipse Plus C18 column with a mobile phase of acetonitrile and water (containing 10mM ammonium acetate) in an isocratic elution way. The mass spectrometry (MS) analysis was conducted in positive ionization mode with multiple reaction monitoring (MRM) transitions at m/z 653.4→411.2 for hernandezine and m/z 623.3→381.3 for tetrandrine (IS). Calibration curves were linear over the ranges of 20.0-4000ng/ml f or both plasma samples and tissue samples (r>0.991). The lower limit of quantification (LLOQ) was 20.0ng/ml. The intra-day and inter-day precision (RSD%) were less than 14.0%, while the accuracy was ranged from 85.2% to 114.9%. Finally, this developed method was successfully applied in the pharmacokinetics and tissue distribution study of hernandezine after intravenous administration.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Song Y,Wang Z,Zhang B,Zhang Y,Zhang W,Yang C,Meng F,Feng Xdoi
10.1016/j.jpba.2017.03.038subject
Has Abstractpub_date
2017-07-15 00:00:00pages
132-139eissn
0731-7085issn
1873-264Xpii
S0731-7085(16)31425-Xjournal_volume
141pub_type
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