Abstract:
:Development of tissue structure and three-dimensional microenvironment is crucial for regeneration of axons in the peripheral nerve repair. In this study we aimed to evaluate the efficacy of nerve regeneration by using an acellular nerve allograft (ANA) injected with allogenic skin-derived precursor differentiated Schwann cells (SKP-SCs) and heregulin-1β. Skin-derived precursor cells (SKPs) were generated from dermis of newborn (postnatal day 2) Wistar rats. In a rat model, nerve regeneration was determined across a 15 mm lesion in the sciatic nerve by using an ANA injected with allogenic SKP-SCs and heregulin-1β. The electrophysiological analysis, histological examination and electron microscopy were involved in this study. Cultured SKPs expressed nestin and fibronectin, and differentiated into cells with phenotype of SCs that presented characteristic markers of p75NGFR and S100-β. Implantation of SKP-SCs into the rat models by using ANA and allogenic skin-derived precursor differentiated Schwann cells (SKP-SCs) increases sciatic nerve functional index (SFI), peak amplitudes, nerve conduction velocities, number of myelinated fibers within the graft, while reduces incubation period, sciatic nerve injury-induced weight and contractions loss. Using ANA injected with SKP-SCs combined with heregulin-1β greatly promote peripheral nerve repair in a rat model. Our results suggest that SKP-SCs transplantation with heregulin-1β represents a powerful therapeutic approach, and facilitates the efficacy of acellular nerve allograft in peripheral nerve injury, though the detailed mechanism remains to be elucidated.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Wang H,Wu J,Zhang X,Ding L,Zeng Qdoi
10.1016/j.neuint.2016.04.003subject
Has Abstractpub_date
2016-07-01 00:00:00pages
146-53eissn
0197-0186issn
1872-9754pii
S0197-0186(16)30052-3journal_volume
97pub_type
杂志文章abstract::Neurosteroids are modulators of several receptors and ion channels and are implicated in the pathophysiology of several neuropsychiatric diseases including hepatic encephalopathy (HE). The neurosteroid, allopregnanolone, a positive allosteric modulator of GABA(A) receptors, accumulates in the brains of HE patients whe...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2005.12.006
更新日期:2006-06-01 00:00:00
abstract::NADPH derived from glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway, has been implicated not only to promote reduced glutathione (GSH) but also enhance oxidative stress in specific cellular conditions. In this study, the effects of G6PD antisense oligodeoxynucleotides...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2012.05.003
更新日期:2012-07-01 00:00:00
abstract::To investigate the contribution of the dopamine (DA) synthesis to both the calcium-dependent and the carrier-mediated, mechanisms of DA release in the striatum, anaesthetized rats were locally superfused in the striatum with a push pull cannula supplied with an artificial CSF containing tritiated tyrosine. DA, dihydro...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(99)00094-7
更新日期:1999-12-01 00:00:00
abstract::Recent data indicate that BMY 7378 demonstrates high affinity, selectivity and low intrinsic activity at hippocampal 5-HT(1A) receptors, suggesting that BMY 7378 may represent the first selective 5-HT(1A) functional antagonist. The present study examined the agonist and antagonist properties of BMY 7378 at spinal cord...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(90)90011-h
更新日期:1990-01-01 00:00:00
abstract::The effects of ?-amino-n-butyric acid (GABA), (+)bicuculline, isoguvacine and 3-(4-chlorophenyl)-4-aminobutyrate [(+/-)baclofen] on the K-induced release of [(3)H]acetylcholine (ACh) were studied in the superior cervical ganglia of the rat in vitro. GABA and isoguvacine inhibited [(3)H]ACh release and these inhibition...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(86)90193-2
更新日期:1986-01-01 00:00:00
abstract::Human neuroblastoma GOTO cell lines were established that stably express recombinant human lysosomal protective protein/cathepsin A (PPCA) cDNA by transfection. Intracellular cathepsin A (acid serine carboxypeptidase) activity increased four-fold compared with in those of the parent and mock-transfected cell lines. Th...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2003.07.001
更新日期:2004-05-01 00:00:00
abstract::Excitotoxicity is the main event during neurological disorders producing drastic morphological and functional changes. Rac-GTPase is involved in cytoskeletal remodeling and survival. However, the role of Rac1 after cerebral ischemia has not been completely understood yet. In this study, we evaluated the activity of Ra...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2010.08.014
更新日期:2010-12-01 00:00:00
abstract::In recent years there has been a great deal of research within the stem cell field which has led to the definition and classification of a range of stem cells from a plethora of tissues and organs. Stem cells, by classification, are considered to be pluri- or multipotent and have both self-renewal and multi-differenti...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2016.08.001
更新日期:2017-06-01 00:00:00
abstract::Tryptophan hydroxylation in cockroach (Periplaneta americana) nervous tissue was measured and compared to the hydroxylation of tryptophan in rat brain. Tryptophan hydroxylation in both tissues requires a pterine cofactor, and is inhibited by p-chlorophenylalanine. The molecular weight of the protein responsible for hy...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(87)90045-3
更新日期:1987-01-01 00:00:00
abstract::A significant loss of dopamine was found in rat striatal slices incubated with 1-methyl-4-phenylpyridinium ion (MPP+) at a concentration of 2 microM or higher. The addition of 7-nitroindazole, a specific inhibitor of neuronal nitric oxide synthase (nNOS), prevented this effect on dopamine when the concentration of MPP...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(05)80006-3
更新日期:1998-07-01 00:00:00
abstract::Diabetic mice exhibit increased mortality and morbidity following stroke. Recent studies from our laboratory have indicated that increased morbidity in diabetic db/db mice relative to their non-diabetic db/+ littermates is associated with increased levels of MMP-9 protease activity, increased blood-brain barrier (BBB)...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2020.104790
更新日期:2020-10-01 00:00:00
abstract::Gamma-aminobutyric acid (GABA) is the main chemical inhibitory neurotransmitter in the brain. In the central nervous system (CNS) it acts on two distinct types of receptor: an ion channel, i.e., an "ionotropic" receptor permeable to Cl- and HCO3- (GABAA receptors) and a G-protein coupled "metabotropic" receptor that i...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2007.07.010
更新日期:2008-01-01 00:00:00
abstract::Control of serotonin release and synthesis by amino acid neurotransmitters was investigated in rat rostral and caudal rhombencephalic raphe cells in primary cultures respectively. Endogenous amounts of taurine, glycine, GABA and glutamate were measured in both types of cultures. These amino acids were spontaneously re...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(93)90118-o
更新日期:1993-09-01 00:00:00
abstract::Although ischemic preconditioning (IP) can provide powerful protection on brain against ischemic insult, it is rarely used in clinic to prevent the occurrence of ischemic stroke because of safety concerns. It is therefore necessary to seek the safer stimuli to initiate pharmacological preconditioning. Our previous wor...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2012.11.007
更新日期:2013-01-01 00:00:00
abstract::The mediatory role of kinins in both acute and chronic inflammation within nervous tissues has been widely described. Bradykinin, the major representative of these bioactive peptides, is one of a few mediators of inflammation that directly stimulates afferent nerves due to the broad expression of specific kinin recept...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2012.04.013
更新日期:2012-07-01 00:00:00
abstract::Two puromycin-sensitive aminopeptidase isozymes (PSA-I and PSA-II) were isolated from chicken brain cytosol by ammonium sulfate fractionation followed by column chromatography on Cellex D and AH-Sepharose 4B and separated on Bio-Gel HTP. Each was purified to homogeneity on Sephadex G-200, Arg-Tyr-AH-Sepharose, Bio-Gel...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(93)90039-8
更新日期:1993-05-01 00:00:00
abstract::The sensitivity of [(3)H]GABA and [(3)H]muscimol high-affinity binding sites to physiological (Krebs-Ringer's bicarbonate) and non-physiological (Tris-citrate) buffers was examined using synaptosomal membranes from bovine retinas. The maximum number of sites (B(max)) for [(3)H]GABA was present when the tissue was assa...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(86)90167-1
更新日期:1986-01-01 00:00:00
abstract::Amyloid β (Aβ) oligomers are presumed to be one of the causes of Alzheimer's disease (AD). Previously, we identified the E693Δ mutation in amyloid precursor protein (APP) in patients with AD who displayed almost no signals of amyloid plaques in amyloid imaging. We generated APP-transgenic mice expressing the E693Δ mut...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2012.05.018
更新日期:2012-08-01 00:00:00
abstract::Recently, a new experimental model of epilepsy was introduced by the authors [Neurochem. Int. 40 (2002) 413]. This model combines pentylenetetrazole (PTZ)-kindling in senescence-accelerated mice P8 (SAMP8), a genetic model of aging. Since imbalance of glutamate and GABA is a major cause of seizures, the study of glial...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(03)00093-7
更新日期:2003-12-01 00:00:00
abstract::The neonatal administration of 5,7-dihydroxytryptamine to rats (100 mg kg(?1) s.c. on the 1st and 2nd day after birth) resulted in marked reductions in serotoninergic presynaptic markers ([(3)H]-5-HT synaptosomal uptake, tryptophan hydroxylase activity and endogenous 5-HT content) in various forebrain areas, particula...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(81)90051-6
更新日期:1981-03-01 00:00:00
abstract::Ketone bodies serve as a source of energy and as precursors for lipid synthesis in developing brain. Neurons, astrocytes and oligodendroglia all have the necessary enzymes for metabolizing ketone bodies. In this in vivo study it was found that all three cell types were able to use ketone bodies as precursors for the s...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(91)90040-k
更新日期:1991-01-01 00:00:00
abstract::In order to elucidate the effects of MPTP on enkephalinergic neurons, dopamine (DA), norepinephrine (NE), proenkephalin (PE) mRNA and met-enkephalin (ME) were measured in striatum, olfactory tubercle, and prefrontal cortex of C57/B16 mice 1 day-2 weeks following treatment with 96 mg/kg MPTP HCl (24 mg/kg i.p., twice/d...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(93)90010-3
更新日期:1993-02-01 00:00:00
abstract::Phosphine-borane complexes are novel cell-permeable drugs that protect neurons from axonal injury in vitro and in vivo. These drugs activate the extracellular signal-regulated kinases 1/2 (ERK1/2) cell survival pathway and are therefore neuroprotective, but do not scavenge superoxide. In order to understand the intera...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2016.05.014
更新日期:2016-10-01 00:00:00
abstract::The short-term influences of stress on the activities of tyrosine hydroxylase in vivo and in vitro were examined in mice. The in vivo tyrosine hydroxylase activity was estimated by the rate of dopa accumulation which was measured at 30 min after the injection of NSD-1015 (100 mg kg), an aromatic l-amino acid decarboxy...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(82)90079-1
更新日期:1982-01-01 00:00:00
abstract::Potassium bisperoxo(1,10-phenantroline)oxovanadate (V) [bpV(phen)] is a potent protein tyrocine phosphatase inhibitor which mediates a variety of biological effects. The aim of these studies was to examine the role(s) of mitogen activated protein kinase (MAPK) pathways in PC12 cell proliferation and toxicity by bpV(ph...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(99)00018-2
更新日期:1999-04-01 00:00:00
abstract:BACKGROUND:Neuronal iron accumulation is thought to be relevant to the pathogenesis of Parkinson's disease (PD), although the mechanism remains elusive. We hypothesized that neuronal iron uptake may be stimulated by functional mitochondrial iron deficiency. OBJECTIVE:To determine firstly whether the mitochondrial toxi...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2011.05.006
更新日期:2011-08-01 00:00:00
abstract::Aging is a biological process characterized by impairment of cellular bioenergetic function, increased oxidative stress, attenuated ability to respond to stresses, increased risk of contracting age-associated disorders that affects many tissues, with a more marked effect on brain and heart function. Oxidative stress i...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2010.12.016
更新日期:2011-03-01 00:00:00
abstract::A sandwich enzyme immunoassay (EIA) was established by using purified human serum dopamine-?-hydroxylase (DBH) as a standard protein and a monospecific polyclonal antibody raised against human DBH purified from human pheochromocytoma. The EIA was applied to measuring DBH levels in human CSF from Parkinsonian patients ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(88)90126-x
更新日期:1988-01-01 00:00:00
abstract::Excessive glucocorticoid levels in depressed patients have been associated with atrophic changes in some brain regions, but only few studies suggest that some antidepressants can interfere with deleterious effect of glucocorticoids on neuronal cells. The aim of the present study was to examine the effect of dexamethas...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2013.07.007
更新日期:2013-11-01 00:00:00
abstract::To identify whether the process of apoptosis bears a topographic relationship to selected aspects of Alzheimer's disease (AD) pathology, we used an in situ nick translation method (TUNEL) to map DNA fragmentation in hippocampal sections immunostained for abnormally phosphorylated tau, which exists in the neurofibrilla...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(96)00158-1
更新日期:1997-08-01 00:00:00