Ginkgolide B preconditioning on astrocytes promotes neuronal survival in ischemic injury via up-regulating erythropoietin secretion.

Abstract:

:Although ischemic preconditioning (IP) can provide powerful protection on brain against ischemic insult, it is rarely used in clinic to prevent the occurrence of ischemic stroke because of safety concerns. It is therefore necessary to seek the safer stimuli to initiate pharmacological preconditioning. Our previous work demonstrated that ginkgolide B (GB) could protect neurons against ischemia-induced apoptosis. Astrocytes are the most numerous cells in mammalian central nervous system and there is a close bi-directional communication between neurons and astrocytes in brain. Besides neurons, whether GB can exert the role of preconditioning on astrocytes through which to further improve neuronal survival under ischemic condition is not yet known. In the present study, primary cultured astrocytes were treated with GB for 24h or short-term ischemia (ischemia for 3h, as ischemic preconditioning/IP), and then cultured back to normoxia and normal medium for 24h to induce the preconditioning response. Astrocyte-conditioned medium (ACM) was then collected and used to incubate the cultured neurons for 24h before neurons were subjected to severe ischemia. Our results demonstrated that not only GB and IP increased astrocytic viability in ischemia, but also the conditioned medium from astrocytes treated with GB or IP increased cell viability and decreased the number of apoptosis of neurons in ischemia. We also found that GB and IP significantly stimulated astrocytes to express and secrete erythropoietin (EPO) into ACM, and the addition of anti-EPO antibody blocked the protective effect of GB or IP-treated astrocytes culture medium on neurons in ischemia. Further study of above protection revealed that ACM from astrocytes treated with GB or IP induced the inactivation of proapoptotic factor Bad by phosphorylation at serine 136 and 112 ((136)p-Bad and (112)p-Bad) in neurons. Together, our results suggest that GB is capable of preconditioning on astrocytes as IP and then protects neurons against ischemia-induced apoptosis, which is mediated by EPO.

journal_name

Neurochem Int

authors

Wu X,Zhou C,Du F,Lu Y,Peng B,Chen L,Zhu L

doi

10.1016/j.neuint.2012.11.007

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

157-64

issue

2

eissn

0197-0186

issn

1872-9754

pii

S0197-0186(12)00367-1

journal_volume

62

pub_type

杂志文章
  • Rac1 activity changes are associated with neuronal pathology and spatial memory long-term recovery after global cerebral ischemia.

    abstract::Excitotoxicity is the main event during neurological disorders producing drastic morphological and functional changes. Rac-GTPase is involved in cytoskeletal remodeling and survival. However, the role of Rac1 after cerebral ischemia has not been completely understood yet. In this study, we evaluated the activity of Ra...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2010.08.014

    authors: Johanna GV,Fredy CA,David VC,Natalia MV,Angel CR,Patricia CG

    更新日期:2010-12-01 00:00:00

  • Differentiation of neuroblastoma cells by chick gizzard extract.

    abstract::Cholinergic neuroblastoma NS20Y cells were differentiated by the chicken gizzard extract. They were first inoculated into a glass culture bottle and the aggregated cells which grew in the suspension culture were collected. The aggregated cells (round and immature neuroblastoma cells) were seeded on a polyornithinecoat...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(82)90026-2

    authors: Miki N,Higashida H,Hayashi Y,Akiyama M,Kuo CH

    更新日期:1982-01-01 00:00:00

  • HEK-293 cells expressing the human dopamine transporter are susceptible to low concentrations of 1-methyl-4-phenylpyridine (MPP+) via impairment of energy metabolism.

    abstract::Selective dopaminergic neurotoxicity induced by 1-methyl-4-phenylpyridine (MPP+) is believed to be due to the transmembrane uptake by the dopamine transporter and subsequent inhibition of mitochondrial complex I and/or production of free radicals. However, little is known about the molecular sequence of intracellular ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(99)00083-2

    authors: Storch A,Ludolph AC,Schwarz J

    更新日期:1999-11-01 00:00:00

  • Protein tyrosine phosphorylation: implications for synaptic function.

    abstract::The phosphorylation of proteins on tyrosine residues, initially believed to be primarily involved in cell growth and differentiation, is now recognized as having a critical role in regulating the function of mature cells. The brain exhibits one of the highest levels of tyrosine kinase activity in the adult animal and ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/s0197-0186(97)00022-3

    authors: Gurd JW

    更新日期:1997-11-01 00:00:00

  • Ventral tegmental area/substantia nigra and prefrontal cortex rodent organotypic brain slices as an integrated model to study the cellular changes induced by oxygen/glucose deprivation and reperfusion: effect of neuroprotective agents.

    abstract::Unveiling the roles of distinct cell types in brain response to insults is a partially unsolved challenge and a key issue for new neuroreparative approaches. In vivo models are not able to dissect the contribution of residential microglia and infiltrating blood-borne monocytes/macrophages, which are fundamentally undi...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2014.01.008

    authors: Colombo L,Parravicini C,Lecca D,Dossi E,Heine C,Cimino M,Wanke E,Illes P,Franke H,Abbracchio MP

    更新日期:2014-01-01 00:00:00

  • Blue light exacerbates and red light counteracts negative insults to retinal ganglion cells in situ and R28 cells in vitro.

    abstract::Neurones are dependent on their mitochondria to produce the necessary amounts of ATP for survival. Retinal ganglion cells (RGCs) have a particularly large number of mitochondria which-unlike neurones in the brain-are exposed to visual light of 400-850 nm. Here we demonstrate that short wavelength visual blue light neg...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2019.02.018

    authors: Núñez-Álvarez C,Osborne NN

    更新日期:2019-05-01 00:00:00

  • Impaired neuronal and astroglial metabolic activity in chronic unpredictable mild stress model of depression: Reversal of behavioral and metabolic deficit with lanicemine.

    abstract::Major depressive disorder is the leading cause of disability and suicidality worldwide. Here, we evaluated neural metabolic activity in prefrontal cortex (PFC) in C57BL6 mice undergoing a chronic unpredictable mild stress (CUMS) for three weeks to induce depression. Further, the efficacy of Lanicemine, a low trapping ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2020.104750

    authors: Mishra PK,Adusumilli M,Deolal P,Mason GF,Kumar A,Patel AB

    更新日期:2020-07-01 00:00:00

  • Abnormal glycosylation of myelin-associated glycoprotein in quaking mouse brain.

    abstract::Brain slices from actively myelinating (26-28 days) quaking and normal littermates were dual-labeled with radioactive mannose and fucose for 2 h. Following the incubation myelin was isolated by sucrose density gradient centrifugation and the incorporation of sugars into the major myelinassociated glycoprotein (MAG) de...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(87)90084-2

    authors: Konat G,Hogan EL,Leskawa KC,Gantt G,Singh I

    更新日期:1987-01-01 00:00:00

  • A novel copper-containing protein from brain.

    abstract::A novel copper-containing protein has been isolated in electrophoretically homogeneous state from bovine brain extracts. Some physico-chemical properties of the protein are determined (molecular weight, copper content, isoelectric point, amino acid composition, optical and EPR spectra), and they are compared with thos...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(88)90071-x

    authors: Mikaelyan MV,Nalbandyan RM

    更新日期:1988-01-01 00:00:00

  • The protective effect of eicosapentaenoic acid-enriched phospholipids from sea cucumber Cucumaria frondosa on oxidative stress in PC12 cells and SAMP8 mice.

    abstract::Alzheimer's disease (AD) is a common neurodegenerative disorders, in which oxidative stress plays an important role. The present study investigated the effect of eicosapentaenoic acid-enriched phospholipids (EPA-enriched PL) from the sea cucumber Cucumaria frondosa on oxidative injury in PC12 cells induced by hydrogen...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2013.10.015

    authors: Wu FJ,Xue Y,Liu XF,Xue CH,Wang JF,Du L,Takahashi K,Wang YM

    更新日期:2014-01-01 00:00:00

  • Parecoxib and its metabolite valdecoxib directly interact with cannabinoid binding sites in CB1-expressing HEK 293 cells and rat brain tissue.

    abstract::Cyclooxygenase 2 inhibitors (COX 2) such as parecoxib (par) and valdecoxib (val) are used in the treatment of neuropathic pain. Using the radioligand binding assay it was demonstrated that both the prodrug par as well as its active metabolite val have a specific affinity to the cannabinoid (CB) receptor measured in CB...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2010.10.018

    authors: Schröder H,Höllt V,Becker A

    更新日期:2011-01-01 00:00:00

  • The neurosteroids, allopregnanolone and progesterone, induce autophagy in cultured astrocytes.

    abstract::Recent studies have suggested that neurosteroids such as pregnenolone, progesterone (PG) and their derivatives, have a role in activating autophagy in addition to diverse other functions. In our previous studies, we demonstrated that cellular free Zn(2+) is involved in oxidative stress-induced autophagy and autophagic...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2011.11.015

    authors: Kim HN,Lee SJ,Koh JY

    更新日期:2012-01-01 00:00:00

  • In vitro neurotransmitter release in an animal model of depression.

    abstract::Sprague-Dawley rats exposed to uncontrollable shock can be separated by a subsequent shock escape test into two groups: a "helpless" (LH) group which demonstrates a deficit in escape behavior, and a "nonlearned helpless" (NLH) group which shows no escape deficit and acquires the escape response as readily as naive con...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(92)90065-y

    authors: Edwards E,Kornrich W,van Houtten P,Henn FA

    更新日期:1992-07-01 00:00:00

  • CNS distribution of D1 receptors: use of a new specific D1 receptor antagonist, [3H]SCH39166.

    abstract::D1 dopamine receptors have been localized using a radioactive form of a new specific antagonist, [3H]SCH39166. This compound has been shown, in in vitro binding studies, to be highly selective for the D1 receptor subtype; more so than its predecessor, [3H]SCH23390. These ligand binds saturably, reversibly and with hig...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/0197-0186(92)90224-f

    authors: Wamsley JK,Alburges ME,McQuade RD,Hunt M

    更新日期:1992-03-01 00:00:00

  • Adult development of the hippocampal-serotonin system of C57BL/6N mice; analysis of high-affinity uptake of (3)H-5HT in slices and synaptosomes.

    abstract::The saturable and specific high-affinity uptake of [(3)H]serotonin ([(3)H]5HT) (5 x 10(?8) M) was studied in slices from the hippocampus, parietal cortex, septum-preoptic area, and hypothalamus of male 2, 6, 12 and 24-32 month old C57BL/6N mice. Hippocampal [(3)H]5-HT uptake showed a significant biphasic relationship ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(83)90006-2

    authors: Azmitia E,Brennan MJ,Quartermain D

    更新日期:1983-01-01 00:00:00

  • Neurotensin concentrations in rat striatum and nucleus accumbens: Further studies of their regulation.

    abstract::The possible influence of cholinergic and dopaminergic mechanisms on neurotensin-containing neurones was examined at two different levels; nucleus accumbens and striatum in the rat brain. The acute treatment with the anticholinergic drugs atropine and scopolamine increased neurotensin concentrations in the striatum an...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(88)90145-3

    authors: Frey P,Lis M,Coward DM

    更新日期:1988-01-01 00:00:00

  • Characterization of cyclothiazide-enhanced kainate excitotoxicity in rat hippocampal cultures.

    abstract::Cyclothiazide has been shown to block desensitization of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-preferring receptors and to enhance quisqualate-, AMPA- and kainate-induced neurotoxicity. The pharmacology behind this cyclothiazide-enhanced kainate-induced excitotoxicity was characterized in em...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(97)00098-3

    authors: Ohno K,Okada M,Tsutsumi R,Matsumoto N,Yamaguchi T

    更新日期:1998-03-01 00:00:00

  • Light exposure stimulates the activity of ganglioside glycosyltransferases of retina ganglion cells.

    abstract::In chicks submitted to light stimulation, the synthesis of gangliosides of the retina ganglion cell increases with respect to chicks maintained in the dark. In an attempt to elucidate if the activation of glycosyltransferases participates in the establishment of these light-dark differences detected in vivo, we examin...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(96)00128-3

    authors: Bussolino DF,Guido ME,Caputto BL

    更新日期:1997-07-01 00:00:00

  • Comparison of [(3)H]-(2S,4R)-4-methylglutamate and [(3)H]D-aspartate as ligands for binding and autoradiographic analyses of glutamate transporters.

    abstract::While studies with [(3)H]D-aspartate ([(3)H]d-Asp) illustrate specific interactions with excitatory amino acid transporters (EAATs), new insights into the pharmacological characteristics and localization of specific EAAT subtypes depend upon the availability of novel ligands. One such ligand is [(3)H]-(2S,4R)-4-methyl...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2007.05.011

    authors: Apricò K,Beart PM,Crawford D,O'shea RD

    更新日期:2007-12-01 00:00:00

  • Neurosteroids: measurement and pathophysiologic relevance.

    abstract::In the last few years, several attempts were made for the setting up of specific methodologies aiming to evaluate steroid contents directly in nervous tissues. The main objective of these attempts was to determine accurately changes intervening in endogenous neuroactive steroid levels during pathological situations. T...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuint.2007.09.010

    authors: Melcangi RC,Mensah-Nyagan AG

    更新日期:2008-03-01 00:00:00

  • Peripheral administration of an anti-TNF-α receptor fusion protein counteracts the amyloid induced elevation of hippocampal TNF-α levels and memory deficits in mice.

    abstract::Alzheimer's disease has long been associated with increased inflammation in the brain. Activated microglia and increased production of the inflammatory cytokines such as TNF-α, have been proposed to contribute to the onset and progression of the disease. We investigated if systemic administration of an anti-tumor necr...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2014.04.001

    authors: Detrait ER,Danis B,Lamberty Y,Foerch P

    更新日期:2014-06-01 00:00:00

  • Brain energy metabolism spurns fatty acids as fuel due to their inherent mitotoxicity and potential capacity to unleash neurodegeneration.

    abstract::The brain uses long-chain fatty acids (LCFAs) to a negligible extent as fuel for the mitochondrial energy generation, in contrast to other tissues that also demand high energy. Besides this generally accepted view, some studies using cultured neural cells or whole brain indicate a moderately active mitochondrial β-oxi...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuint.2017.03.018

    authors: Schönfeld P,Reiser G

    更新日期:2017-10-01 00:00:00

  • Alzheimer's disease as oligomeropathy.

    abstract::Alzheimer's disease (AD) is the most common age-related neurodegenerative disorder and is characterized by pathological aggregates of amyloid β-protein (Aβ) and tau protein. On the basis of genetic evidence, biochemical data, and animal models, Aβ has been suggested to be responsible for the pathogenesis of AD (the am...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuint.2017.08.010

    authors: Ono K

    更新日期:2018-10-01 00:00:00

  • Presenilin-1 and the amyloid precursor protein are transported bidirectionally in the sciatic nerve of adult rat.

    abstract::The amyloid precursor protein (APP) and presenilin-1 (PS-1) are not only of importance for the normal functioning of the various neurons, but also play central roles in the pathogenesis of Alzheimer's disease (AD). Through the use of immunohistochemical and Western blot techniques, the bidirectional axonal transport o...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/s0197-0186(02)00014-1

    authors: Papp H,Pakaski M,Kasa P

    更新日期:2002-12-01 00:00:00

  • Solar light induces expression of acetylcholinesterase in skin keratinocytes: Signalling mediated by activator protein 1 transcription factor.

    abstract::Acetylcholinesterase (AChE) hydrolyses acetylcholine to choline and acetate, playing an important role in terminating the neurotransmission in brain and muscle. Recently, the non-neuronal functions of AChE have been proposed in different tissues, in which there are various factors to regulate the expression of AChE. I...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2020.104861

    authors: Wu Q,Bai P,Xia Y,Lai QWS,Guo MSS,Dai K,Zheng Z,Ling CSJ,Dong TTX,Pi R,Tsim KWK

    更新日期:2020-12-01 00:00:00

  • Unchanged glutamine synthetase activity and increased NMDA receptor density in epileptic human neocortex: implications for the pathophysiology of epilepsy.

    abstract::We investigated whether alterations in glutamate metabolising glutamine synthetase activity occur in human epileptic neocortex, as shown previously for human epileptic hippocampus [Eid, T., Thomas, M.J., Spencer, D.D., Rundén-Pran, E., Lai, J.C.K., Malthankar, G.V., Kim, J.H., Danbolt, N.C., Ottersen, O.P., de Lanerol...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2005.06.001

    authors: Steffens M,Huppertz HJ,Zentner J,Chauzit E,Feuerstein TJ

    更新日期:2005-11-01 00:00:00

  • Neutrophil granulocytes in cerebral ischemia - Evolution from killers to key players.

    abstract::Neutrophil granulocytes (or polymorphonuclear cells, PMNs) have long been considered as crude killing machines, particularly trained to attack bacterial or fungal pathogens in wounds or infected tissues. That perspective has fundamentally changed over the last decades, as PMNs have been shown to exert a livery exchang...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuint.2016.11.006

    authors: Strecker JK,Schmidt A,Schäbitz WR,Minnerup J

    更新日期:2017-07-01 00:00:00

  • Different roles of adenosine A1, A2A and A3 receptors in controlling kainate-induced toxicity in cortical cultured neurons.

    abstract::Adenosine is a neuromodulator that can control brain damage through activation of A(1), A(2A) and A(3) receptors, which are located in both neurons and other brain cells. We took advantage of cultured neurons to investigate the role of neuronal adenosine receptors in the control of neurotoxicity caused by kainate and ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/j.neuint.2005.05.009

    authors: Rebola N,Rodrigues RJ,Oliveira CR,Cunha RA

    更新日期:2005-10-01 00:00:00

  • Astrocyte functions in the copper homeostasis of the brain.

    abstract::Copper is an essential element that is required for a variety of important cellular functions. Since not only copper deficiency but also excess of copper can seriously affect cellular functions, the cellular copper metabolism is tightly regulated. In brain, astrocytes appear to play a pivotal role in the copper metabo...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuint.2012.08.017

    authors: Scheiber IF,Dringen R

    更新日期:2013-04-01 00:00:00

  • Effects of nigral dopaminergic lesions and striatal excitotoxin lesions on brain converting enzyme.

    abstract::High levels of angiotensin converting enzyme (ACE) are found in the caudate-putamen (CPu), globus pallidus, entopeduncular nucleus and substantia nigra pars reticulata; these structures were connected by a continuous band of ACE. In order to investigate whether ACE was associated with dopaminergic projections from the...

    journal_title:Neurochemistry international

    pub_type: 杂志文章

    doi:10.1016/0197-0186(87)90180-x

    authors: Chai SY,Christie MJ,Beart PM,Mendelsohn FA

    更新日期:1987-01-01 00:00:00