A Novel qPCR Method for Simultaneous Detection and Quantification of Viable Pathogenic and Non-pathogenic Vibrio parahaemolyticus (tlh+ , tdh+ , and ureR + ).

Abstract:

:Pathogenic and non-pathogenic Vibrio parahaemolyticus strains were simultaneously detected and quantified using a novel viable multiplex real-time PCR (novel qPCR). We used a new PCR primer and probe, ureR, as a surrogate for detection of the toxin trh gene as the primer was better at identifying variant V. parahaemolyticus trh strains. The specificity of all primers and probes used in this study were validated on three standard strains of V. parahaemolyticus, 42 clinical strains, 12 wild strains, 4 strains of Vibrio spp., and 4 strains of other bacteria. Then, propidium monoazide (PMA) was applied to inhibit DNA of dead cell, and the results of PMA optimized treatments were 15 μM concentration, 5 min incubation periods, 15 min light exposure periods and 30 RPM rotational speed, which resulted in time and cost savings. Pathogenic and non-pathogenic strains were quantified using a two-reaction tube method where the tlh, tdh, and ureR genes were amplified. Additionally, standard curves with a 7-log dynamic range were generated for quantifying viable V. parahaemolyticus and the amplification efficiencies were 108.68, 105.17, and 115.61% for tlh+ , tdh+ , and ureR + . This novel qPCR accurately monitored V. parahaemolyticus contamination rates in shrimps (Penaeus vannamei) and clams (Ruditapes philippinarum) sampled from retail stores located in a major district in Shanghai. In conclusion, our assay can prioritize the detection and quantification of viable pathogenic V. parahaemolyticus and can prove to be a more effective tool for reducing infection risks from consumption of seafood in Shanghai.

journal_name

Front Microbiol

authors

Niu B,Hong B,Zhang Z,Mu L,Malakar PK,Liu H,Pan Y,Zhao Y

doi

10.3389/fmicb.2018.01747

subject

Has Abstract

pub_date

2018-08-02 00:00:00

pages

1747

issn

1664-302X

journal_volume

9

pub_type

杂志文章
  • Colistin Resistance Gene mcr-1 Mediates Cell Permeability and Resistance to Hydrophobic Antibiotics.

    abstract::Colistin is considered the last-resort antibiotic used to treat multidrug resistant bacteria-related infections. However, the discovery of the plasmid-mediated colistin resistance gene, mcr-1, threatens the clinical utility of colistin antibiotics. In this study, the physiological function of MCR-1, which encodes an L...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03015

    authors: Li B,Yin F,Zhao X,Guo Y,Wang W,Wang P,Zhu H,Yin Y,Wang X

    更新日期:2020-01-10 00:00:00

  • Maturation Mechanism of Nitrile Hydratase From Streptomyces canus CGMCC 13662 and Its Structural Character.

    abstract::Nitrile hydratases have received significant interest both in the large-scale industrial production of acrylamide and nicotinamide, and the remediation of environmental contamination with nitrile-containing pollutants. Almost all known nitrile hydratases include an α-subunit (AnhA) and β-subunit (AnhB), and a specific...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01419

    authors: Guo L,Cheng X,Jiang HY,Dai YJ

    更新日期:2020-06-25 00:00:00

  • Degradation of Chrysene by Enriched Bacterial Consortium.

    abstract::Chrysene is a high molecular weight (HMW), polycyclic aromatic hydrocarbon (PAH) known for its recalcitrance and carcinogenic properties and sparsely soluble (0.003 mg/L) in aqueous medium. Due to these refractory properties, bioavailability of chrysene is very low and therefore is persistence in the environment escap...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01333

    authors: Vaidya S,Devpura N,Jain K,Madamwar D

    更新日期:2018-06-26 00:00:00

  • Stochastic Individual-Based Modeling of Bacterial Growth and Division Using Flow Cytometry.

    abstract::A realistic description of the variability in bacterial growth and division is critical to produce reliable predictions of safety risks along the food chain. Individual-based modeling of bacteria provides the theoretical framework to deal with this variability, but it requires information about the individual behavior...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02626

    authors: García MR,Vázquez JA,Teixeira IG,Alonso AA

    更新日期:2018-01-05 00:00:00

  • Bacillus amyloliquefaciens Ameliorates Dextran Sulfate Sodium-Induced Colitis by Improving Gut Microbial Dysbiosis in Mice Model.

    abstract::Several Bacillus strains exert beneficial effects on the maintenance of intestinal homeostasis and host health. However, whether Bacillus amyloliquefaciens (BA) can improve gut microbial dysbiosis and ameliorate colitis is unknown. Therefore, we conducted the present study to investigate the effects of BA administrati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03260

    authors: Cao G,Wang K,Li Z,Tao F,Xu Y,Lan J,Chen G,Yang C

    更新日期:2019-01-08 00:00:00

  • WadD, a New Brucella Lipopolysaccharide Core Glycosyltransferase Identified by Genomic Search and Phenotypic Characterization.

    abstract::Brucellosis, an infectious disease caused by Brucella, is one of the most extended bacterial zoonosis in the world and an important cause of economic losses and human suffering. The lipopolysaccharide (LPS) of Brucella plays a major role in virulence as it impairs normal recognition by the innate immune system and del...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02293

    authors: Salvador-Bescós M,Gil-Ramírez Y,Zúñiga-Ripa A,Martínez-Gómez E,de Miguel MJ,Muñoz PM,Cloeckaert A,Zygmunt MS,Moriyón I,Iriarte M,Conde-Álvarez R

    更新日期:2018-09-27 00:00:00

  • The Putative Methyltransferase TlLAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2.

    abstract::The biocontrol fungus Trichoderma longibrachiatum SMF2 secretes a large quantity of peptaibols that have been shown to have a range of biological activities and therefore great application values. However, the mechanism of the regulatory expression of peptaibols is still unclear. The putative methyltransferase LaeA/LA...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01267

    authors: Shi JC,Shi WL,Zhou YR,Chen XL,Zhang YZ,Zhang X,Zhang WX,Song XY

    更新日期:2020-06-12 00:00:00

  • Dynamics in the Strawberry Rhizosphere Microbiome in Response to Biochar and Botrytis cinerea Leaf Infection.

    abstract::Adding biochar, the solid coproduct of biofuel production, to peat can enhance strawberry growth, and disease resistance against the airborne fungal pathogen Botrytis cinerea. Additionally, biochar can induce shifts in the strawberry rhizosphere microbiome. However, the moment that this biochar-mediated shift occurs i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.02062

    authors: De Tender C,Haegeman A,Vandecasteele B,Clement L,Cremelie P,Dawyndt P,Maes M,Debode J

    更新日期:2016-12-22 00:00:00

  • Prevalence and Antibiotic Resistance of Non-typhoidal Salmonella Isolated from Raw Chicken Carcasses of Commercial Broilers and Spent Hens in Tai'an, China.

    abstract::The present study was aimed to determine the prevalence and characteristics of Salmonella isolated from meat samples of commercial broilers (CB) and spent hens (SH). Between March and June 2016, 200 retail raw chicken carcasses (100 from CB and 100 from SH) were obtained from local supermarkets in Tai'an city of China...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02106

    authors: Li S,Zhou Y,Miao Z

    更新日期:2017-10-31 00:00:00

  • Genetic Basis and Clonal Population Structure of Antibiotic Resistance in Campylobacter jejuni Isolated From Broiler Carcasses in Belgium.

    abstract::Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of Campylobacter jejuni strains isolated from broiler carcasses in Belgiu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01014

    authors: Elhadidy M,Miller WG,Arguello H,Álvarez-Ordóñez A,Duarte A,Dierick K,Botteldoorn N

    更新日期:2018-05-17 00:00:00

  • Exposure to Antibiotics Affects Saponin Immersion-Induced Immune Stimulation and Shift in Microbial Composition in Zebrafish Larvae.

    abstract::In the last decades, pollution of the environment by large scale use of antibiotics in agriculture and human medicine have led to increased antimicrobial resistance in both the environment and the host animal microbiome. Disturbances in the host microbiome can result in impaired immunity and reduced resilience of aqua...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02588

    authors: López Nadal A,Peggs D,Wiegertjes GF,Brugman S

    更新日期:2018-10-29 00:00:00

  • Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage.

    abstract::F-specific filamentous phage of Escherichia coli (Ff: f1, M13, or fd) are long thin filaments (860 nm × 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00316

    authors: Sattar S,Bennett NJ,Wen WX,Guthrie JM,Blackwell LF,Conway JF,Rakonjac J

    更新日期:2015-04-20 00:00:00

  • Increased Prevalence of Human Polyomavirus JC Viruria in Chronic Inflammatory Rheumatic Diseases Patients in Treatment with Anti-TNF α: A 18 Month Follow-Up Study.

    abstract::Chronic inflammatory rheumatic diseases (CIRDs) are immune-mediated pathologies involving joints. To date, TNFα-blocking agents administration is the most promising therapy, although these treatments are associated with an increased Polyomavirus JC (JCPyV) reactivation, the etiological agent of the Progressive Multifo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00672

    authors: Rodio DM,Anzivino E,Mischitelli M,Bellizzi A,Scrivo R,Scribano D,Conte G,Prezioso C,Trancassini M,Valesini G,Palamara AT,Pietropaolo V

    更新日期:2016-05-10 00:00:00

  • Transcriptomics Indicates Active and Passive Metronidazole Resistance Mechanisms in Three Seminal Giardia Lines.

    abstract::Giardia duodenalis is an intestinal parasite that causes 200-300 million episodes of diarrhoea annually. Metronidazole (Mtz) is a front-line anti-giardial, but treatment failure is common and clinical resistance has been demonstrated. Mtz is thought to be activated within the parasite by oxidoreductase enzymes, and to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00398

    authors: Ansell BR,Baker L,Emery SJ,McConville MJ,Svärd SG,Gasser RB,Jex AR

    更新日期:2017-03-17 00:00:00

  • The Role of Candida albicans Secreted Polysaccharides in Augmenting Streptococcus mutans Adherence and Mixed Biofilm Formation: In vitro and in vivo Studies.

    abstract::The oral cavity is a complex environment harboring diverse microbial species that often co-exist within biofilms formed on oral surfaces. Within a biofilm, inter-species interactions can be synergistic in that the presence of one organism generates a niche for another enhancing colonization. Among these species are th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00307

    authors: Khoury ZH,Vila T,Puthran TR,Sultan AS,Montelongo-Jauregui D,Melo MAS,Jabra-Rizk MA

    更新日期:2020-02-28 00:00:00

  • Precise Fecal Microbiome of the Herbivorous Tibetan Antelope Inhabiting High-Altitude Alpine Plateau.

    abstract::The metataxonomic approach combining 16S rRNA gene amplicon sequencing using the PacBio Technology with the application of the operational phylogenetic unit (OPU) approach, has been used to analyze the fecal microbial composition of the high-altitude and herbivorous Tibetan antelopes. The fecal samples of the antelope...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02321

    authors: Bai X,Lu S,Yang J,Jin D,Pu J,Díaz Moyá S,Xiong Y,Rossello-Mora R,Xu J

    更新日期:2018-09-28 00:00:00

  • Novel plant inputs influencing Ralstonia solanacearum during infection.

    abstract::Ralstonia solanacearum is a soil and water-borne pathogen that can infect a wide range of plants and cause the devastating bacterial wilt disease. To successfully colonize a host, R. solanacearum requires the type III secretion system (T3SS), which delivers bacterial effector proteins inside the plant cells. HrpG is a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00349

    authors: Zuluaga AP,Puigvert M,Valls M

    更新日期:2013-11-20 00:00:00

  • Growth and membrane fluidity of food-borne pathogen Listeria monocytogenes in the presence of weak acid preservatives and hydrochloric acid.

    abstract::This study addresses a major issue in microbial food safety, the elucidation of correlations between acid stress and changes in membrane fluidity of the pathogen Listeria monocytogenes. In order to assess the possible role that membrane fluidity changes play in L. monocytogenes tolerance to antimicrobial acids (acetic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00152

    authors: Diakogiannis I,Berberi A,Siapi E,Arkoudi-Vafea A,Giannopoulou L,Mastronicolis SK

    更新日期:2013-06-14 00:00:00

  • Corrigendum: Cross-Talk between Staphylococcus aureus and Other Staphylococcal Species via the agr Quorum Sensing System.

    abstract::[This corrects the article on p. 1733 in vol. 7, PMID: 27877157.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2017.01075

    authors: Canovas J,Baldry M,Bojer MS,Andersen PS,Grzeskowiak PK,Stegger M,Damborg P,Olsen CA,Ingmer H

    更新日期:2017-06-08 00:00:00

  • Three Capsular Polysaccharide Synthesis-Related Glucosyltransferases, GT-1, GT-2 and WcaJ, Are Associated With Virulence and Phage Sensitivity of Klebsiella pneumoniae.

    abstract::Klebsiella pneumoniae (K. pneumoniae) spp. are important nosocomial and community-acquired opportunistic pathogens, which cause various infections. We observed that K. pneumoniae strain K7 abruptly mutates to rough-type phage-resistant phenotype upon treatment with phage GH-K3. In the present study, the rough-type pha...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01189

    authors: Cai R,Wang G,Le S,Wu M,Cheng M,Guo Z,Ji Y,Xi H,Zhao C,Wang X,Xue Y,Wang Z,Zhang H,Fu Y,Sun C,Feng X,Lei L,Yang Y,Ur Rahman S,Liu X,Han W,Gu J

    更新日期:2019-05-28 00:00:00

  • Alternatives to Antibiotics to Prevent Necrotic Enteritis in Broiler Chickens: A Microbiologist's Perspective.

    abstract::Since the 2006 European ban on the use of antibiotics as growth promoters in animal feed, numerous studies have been published describing alternative strategies to prevent diseases in animals. A particular focus has been on prevention of necrotic enteritis in poultry caused by Clostridium perfringens by the use of mic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.01336

    authors: Caly DL,D'Inca R,Auclair E,Drider D

    更新日期:2015-12-01 00:00:00

  • Induction of Escherichia coli Into a VBNC State by Continuous-Flow UVC and Subsequent Changes in Metabolic Activity at the Single-Cell Level.

    abstract::A viable but non-culturable (VBNC) state of bacteria induced by disinfection in water treatment poses serious health risks because of possible resuscitation of VBNC cells during transportation. In this study, a setup using continuous-flow ultraviolet (UVC) irradiation ranging from 0 to 172.2 mJ cm-2 was designed to si...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02243

    authors: Zhang S,Guo L,Yang K,Zhang Y,Ye C,Chen S,Yu X,Huang WE,Cui L

    更新日期:2018-09-25 00:00:00

  • Rare Plankton Subcommunities Are Far More Affected by DNA Extraction Kits Than Abundant Plankton.

    abstract::Advances in high-throughput sequencing technologies allow a more complete study of microbial plankton community composition and diversity, especially in the rare microbial biosphere. The DNA extraction of plankton is a key step for such studies; however, little is known about its influences on the abundant or rare mic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00454

    authors: Liu M,Xue Y,Yang J

    更新日期:2019-03-11 00:00:00

  • The distribution of thiamin and pyridoxine in the western tropical North Atlantic Amazon River plume.

    abstract::B-vitamins are recognized as essential organic growth factors for many organisms, although little is known about their abundance and distribution in marine ecosystems. Despite their metabolic functions regulating important enzymatic reactions, the methodology to directly measure different B-vitamins in aquatic environ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00025

    authors: Barada LP,Cutter L,Montoya JP,Webb EA,Capone DG,Sañudo-Wilhelmy SA

    更新日期:2013-03-07 00:00:00

  • Antibiotic Resistance Patterns of Pseudomonas spp. Isolated from the River Danube.

    abstract::Spread and persistence of antibiotic resistance pose a severe threat to human health, yet there is still lack of knowledge about reservoirs of antibiotic resistant bacteria in the environment. We took the opportunity of the Joint Danube Survey 3 (JDS3), the world's biggest river research expedition of its kind in 2013...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00586

    authors: Kittinger C,Lipp M,Baumert R,Folli B,Koraimann G,Toplitsch D,Liebmann A,Grisold AJ,Farnleitner AH,Kirschner A,Zarfel G

    更新日期:2016-05-03 00:00:00

  • Anchors for effectors: subversion of phosphoinositide lipids by legionella.

    abstract::The facultative intracellular pathogen Legionella pneumophila replicates in free-living amoebae and macrophages within a distinct compartment, the "Legionella-containing vacuole" (LCV). LCV formation involves phosphoinositide (PI) glycerolipids, which are key factors controlling vesicle trafficking pathways and membra...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00091

    authors: Hilbi H,Weber S,Finsel I

    更新日期:2011-04-27 00:00:00

  • Molecular Analysis of S-morphology Aflatoxin Producers From the United States Reveals Previously Unknown Diversity and Two New Taxa.

    abstract::Aflatoxins are highly toxic carcinogens that detrimentally influence profitability of agriculture and the health of humans and domestic animals. Several phylogenetically distinct fungi within Aspergillus section Flavi have S-morphology (average sclerotial size < 400 μm), and consistently produce high concentrations of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01236

    authors: Singh P,Callicott KA,Orbach MJ,Cotty PJ

    更新日期:2020-06-11 00:00:00

  • The Nuclear-Localized RxLR Effector PvAvh74 From Plasmopara viticola Induces Cell Death and Immunity Responses in Nicotiana benthamiana.

    abstract::Downy mildew is one of the most serious diseases of grapevine (Vitis spp). The causal agent of grapevine downy mildew, Plasmopara viticola, is an obligate biotrophic oomycete. Although oomycete pathogens such as P. viticola are known to secrete RxLR effectors to manipulate host immunity, there have been few studies of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01531

    authors: Yin X,Shang B,Dou M,Liu R,Chen T,Xiang G,Li Y,Liu G,Xu Y

    更新日期:2019-07-10 00:00:00

  • The Gut Microbiome as a Major Regulator of the Gut-Skin Axis.

    abstract::The adult intestine hosts a myriad of diverse bacterial species that reside mostly in the lower gut maintaining a symbiosis with the human habitat. In the current review, we describe the neoteric advancement in our comprehension of how the gut microbiota communicates with the skin as one of the main regulators in the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.01459

    authors: Salem I,Ramser A,Isham N,Ghannoum MA

    更新日期:2018-07-10 00:00:00

  • Integration of PK/PD for dose optimization of Cefquinome against Staphylococcus aureus causing septicemia in cattle.

    abstract::Cefquinome is a fourth generation cephalosporin with antimicrobial activity against gram negative and gram positive bacterial species, including Staphylococcus aureus. The aim of our study was to observe the ex-vivo activity of cefquinome against Staphylococcus aureus strains by using bovine serum from intravenously t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00588

    authors: Ahmad I,Hao H,Huang L,Sanders P,Wang X,Chen D,Tao Y,Xie S,Xiuhua K,Li J,Dan W,Yuan Z

    更新日期:2015-06-17 00:00:00