Adenosine Triphosphate Release is Required for Toll-Like Receptor-Induced Monocyte/Macrophage Activation, Inflammasome Signaling, Interleukin-1β Production, and the Host Immune Response to Infection.

Abstract:

OBJECTIVES:Monocytes and macrophages produce interleukin-1β by inflammasome activation which involves adenosine triphosphate release, pannexin-1 channels, and P2X7 receptors. However, interleukin-1β can also be produced in an inflammasome-independent fashion. Here we studied if this mechanism also involves adenosine triphosphate signaling and how it contributes to inflammasome activation. DESIGN:In vitro studies with human cells and randomized animal experiments. SETTING:Preclinical academic research laboratory. SUBJECTS:Wild-type C57BL/6 and pannexin-1 knockout mice, healthy human subjects for cell isolation. INTERVENTIONS:Human monocytes and U937 macrophages were treated with different inhibitors to study how purinergic signaling contributes to toll-like receptor-induced cell activation and interleukin-1β production. Wild-type and pannexin-1 knockout mice were subjected to cecal ligation and puncture to study the role of purinergic signaling in interleukin-1β production and host immune defense. MEASUREMENTS AND MAIN RESULTS:Toll-like receptor agonists triggered mitochondrial adenosine triphosphate production and adenosine triphosphate release within seconds. Inhibition of mitochondria, adenosine triphosphate release, or P2 receptors blocked p38 mitogen-activated protein kinase and caspase-1 activation and interleukin-1β secretion. Mice lacking pannexin-1 failed to activate monocytes, to produce interleukin-1β, and to effectively clear bacteria following cecal ligation and puncture. CONCLUSIONS:Purinergic signaling has two separate roles in monocyte/macrophage activation, namely to facilitate the initial detection of danger signals via toll-like receptors and subsequently to regulate nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3 inflammasome activation. Further dissection of these mechanisms may reveal novel therapeutic targets for immunomodulation in critical care patients.

journal_name

Crit Care Med

journal_title

Critical care medicine

authors

Lee AH,Ledderose C,Li X,Slubowski CJ,Sueyoshi K,Staudenmaier L,Bao Y,Zhang J,Junger WG

doi

10.1097/CCM.0000000000003446

subject

Has Abstract

pub_date

2018-12-01 00:00:00

pages

e1183-e1189

issue

12

eissn

0090-3493

issn

1530-0293

journal_volume

46

pub_type

杂志文章
  • RBC transfusion practices among critically ill patients: has evidence changed practice?

    abstract:OBJECTIVE:Increasing evidence, including publication of the Transfusion Requirements in Critical Care trial in 1999, supports a lower hemoglobin threshold for RBC transfusion in ICU patients. However, little is known regarding the influence of this evidence on clinical practice over time in a large population-based coh...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/CCM.0b013e31828e9a49

    authors: Murphy DJ,Needham DM,Netzer G,Zeger SL,Colantuoni E,Ness P,Pronovost PJ,Berenholtz SM

    更新日期:2013-10-01 00:00:00

  • Ventilation/perfusion indices do not correlate with the difference between oxygen consumption measured by the Fick principle and metabolic monitoring systems in critically ill patients.

    abstract:OBJECTIVE:To determine whether the difference between oxygen consumption (VO2) measured by metabolic gas monitoring systems and by the Fick principle is related to venous admixture, deadspace/tidal volume ratio, or alveolar-arterial oxygen tension gradient in critically ill patients. DESIGN:A prospective study. SETTI...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-199204000-00008

    authors: Myburgh JA,Webb RK,Worthley LI

    更新日期:1992-04-01 00:00:00

  • Implementation and outcomes of the Multiple Urgent Sepsis Therapies (MUST) protocol.

    abstract:OBJECTIVES:To describe the effectiveness of a comprehensive, interdisciplinary sepsis treatment protocol with regard to both implementation and outcomes and to compare the mortality rates and therapies of patients with septic shock with similar historical controls. DESIGN:Prospective, interventional cohort study with ...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/01.CCM.0000206104.18647.A8

    authors: Shapiro NI,Howell MD,Talmor D,Lahey D,Ngo L,Buras J,Wolfe RE,Weiss JW,Lisbon A

    更新日期:2006-04-01 00:00:00

  • Venous Thromboembolism Events Following Venovenous Extracorporeal Membrane Oxygenation for Severe Acute Respiratory Syndrome Coronavirus 2 Based on CT Scans.

    abstract:OBJECTIVES:The main objective of the study was to determine the prevalence of venous thromboembolism events in patients infected with severe acute respiratory syndrome coronavirus 2 requiring venovenous extracorporeal membrane oxygenation. The secondary objective was to compare venous thromboembolism events and coagula...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/CCM.0000000000004504

    authors: Parzy G,Daviet F,Puech B,Sylvestre A,Guervilly C,Porto A,Hraiech S,Chaumoitre K,Papazian L,Forel JM

    更新日期:2020-10-01 00:00:00

  • Anemia and hematoma volume in acute intracerebral hemorrhage.

    abstract:OBJECTIVE:Anemia increases risk of bleeding complications in the critically ill. In primary intracerebral hemorrhage (ICH), the most fatal type of stroke, outcome is largely dependent on the volume of hemorrhage into the brain. We investigated the relationship between anemia and clinical course of acute ICH. METHODS:S...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/CCM.0b013e31819ced3a

    authors: Kumar MA,Rost NS,Snider RW,Chanderraj R,Greenberg SM,Smith EE,Rosand J

    更新日期:2009-04-01 00:00:00

  • Ventilator-Associated Events: Prevalence, Outcome, and Relationship With Ventilator-Associated Pneumonia.

    abstract:OBJECTIVES:Centers for Disease Control and Prevention built up new surveillance paradigms for the patients on mechanical ventilation and the ventilator-associated events, comprising ventilator-associated conditions and infection-related ventilator-associated complications. We assess 1) the current epidemiology of venti...

    journal_title:Critical care medicine

    pub_type: 杂志文章,多中心研究

    doi:10.1097/CCM.0000000000001091

    authors: Bouadma L,Sonneville R,Garrouste-Orgeas M,Darmon M,Souweine B,Voiriot G,Kallel H,Schwebel C,Goldgran-Toledano D,Dumenil AS,Argaud L,Ruckly S,Jamali S,Planquette B,Adrie C,Lucet JC,Azoulay E,Timsit JF,OUTCOMEREA Study

    更新日期:2015-09-01 00:00:00

  • Trendelenburg position and passive leg raising do not significantly improve cardiopulmonary performance in the anesthetized patient with coronary artery disease.

    abstract::The effects of the Trendelenburg (TREND) position and passive straight leg raising (PLR) on cardiopulmonary performance in 18 anesthetized patients undergoing myocardial revascularization were studied with a two-dimensional transesophageal echocardiography probe and a thermodilution right ventricular ejection fraction...

    journal_title:Critical care medicine

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00003246-198904000-00003

    authors: Reich DL,Konstadt SN,Raissi S,Hubbard M,Thys DM

    更新日期:1989-04-01 00:00:00

  • Antifibrillatory and electrophysiologic actions of moricizine alone and in combination with lidocaine: a prospective, randomized trial.

    abstract:OBJECTIVE:The Cardiac Arrhythmia Suppression Trial II showed that moricizine acutely increases the occurrence of sudden cardiac death. Thus the objective of this investigation was to evaluate the antifibrillatory properties of moricizine (a new antiarrhythmic agent) alone and in combination with lidocaine (an establish...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-199310000-00029

    authors: Ujhelyi MR,O'Rangers EA,Fan C,Kluger J,Chow MS

    更新日期:1993-10-01 00:00:00

  • Exacerbation by granulocyte colony-stimulating factor of prior acute lung injury: implication of neutrophils.

    abstract:OBJECTIVE:Granulocyte colony-stimulating factor is widely prescribed to hasten recovery from cancer chemotherapy-induced neutropenia and has been reported to induce pulmonary toxicity. However, circumstances and mechanisms of this toxicity remain poorly known. DESIGN:To reproduce a routine situation in cancer patients...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-200209000-00027

    authors: Azoulay E,Attalah H,Yang K,Jouault H,Schlemmer B,Brun-Buisson C,Brochard L,Harf A,Delclaux C

    更新日期:2002-09-01 00:00:00

  • Ketanserin in the treatment of pulmonary hypertension after valvular surgery: a comparison with sodium nitroprusside.

    abstract::In a prospective randomized trial in patients with a history of preoperative pulmonary hypertension who were undergoing surgery for valvular replacement or annuloplasty, the effects of ketanserin (KET) (12 patients) and sodium nitroprusside (SNP) (14 patients) on the systemic and pulmonary circulation and pulmonary sh...

    journal_title:Critical care medicine

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00003246-198907000-00003

    authors: van der Starre PJ,Feld RJ,Reneman RS

    更新日期:1989-07-01 00:00:00

  • Cardioprotection, attenuated systemic inflammation, and survival benefit of beta1-adrenoceptor blockade in severe sepsis in rats.

    abstract:OBJECTIVE:To explore the hypothesis that beta-1 adrenoreceptor blockade may be protective through the attenuation of sympathetic hyperactivity and catecholaminergic inflammatory effects on cardiac and hepatic function. DESIGN:Prospective, randomized, controlled study. SETTING:Animal laboratory in a university medical...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/CCM.0b013e3181c03dfa

    authors: Ackland GL,Yao ST,Rudiger A,Dyson A,Stidwill R,Poputnikov D,Singer M,Gourine AV

    更新日期:2010-02-01 00:00:00

  • Topical inhibition of nuclear factor-kappaB enhances reduction in lung edema by surfactant in a piglet model of airway lavage.

    abstract:OBJECTIVE:Acute respiratory distress syndrome is occasionally seen in newborn infants due to a severe inflammatory process in the lungs that affects capillary-alveolar permeability, epithelial integrity, and type I and II pneumocyte function. The aim of this study was to investigate the effect of a topically applied nu...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/01.ccm.0000166371.18066.5e

    authors: Ankermann T,Reisner A,Wiemann T,Krams M,Köhler H,Krause MF

    更新日期:2005-06-01 00:00:00

  • The Epidemiology of Chronic Critical Illness After Severe Traumatic Injury at Two Level-One Trauma Centers.

    abstract:OBJECTIVE:To determine the incidence and risk factors of chronic critical illness after severe blunt trauma. DESIGN:Prospective observational cohort study (NCT01810328). SETTING:Two level-one trauma centers in the United States. PATIENTS:One hundred thirty-five adult blunt trauma patients with hemorrhagic shock who ...

    journal_title:Critical care medicine

    pub_type: 杂志文章,多中心研究

    doi:10.1097/CCM.0000000000002697

    authors: Mira JC,Cuschieri J,Ozrazgat-Baslanti T,Wang Z,Ghita GL,Loftus TJ,Stortz JA,Raymond SL,Lanz JD,Hennessy LV,Brumback B,Efron PA,Baker HV,Moore FA,Maier RV,Moldawer LL,Brakenridge SC

    更新日期:2017-12-01 00:00:00

  • A potential role of hyperbaric oxygen exposure through intestinal nuclear factor-kappaB.

    abstract:OBJECTIVE:Recent studies have demonstrated the therapeutic effectiveness and pharmacologic mechanisms of hyperbaric oxygen therapy (HBOT) in the treatment of a systemic shock state. To elucidate the in vivo role of HBOT during sepsis, we evaluated the effects of HBOT on intestinal mucosal injury and bacterial transloca...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/01.ccm.0000132898.27101.6c

    authors: Sakoda M,Ueno S,Kihara K,Arikawa K,Dogomori H,Nuruki K,Takao S,Aikou T

    更新日期:2004-08-01 00:00:00

  • Critical care for the severely ill head and neck patient.

    abstract:OBJECTIVE:To delineate the frequency and causes of admission to a critical care environment for patients undergoing head and neck surgery at Memorial Sloan-Kettering Cancer Center. DESIGN:Retrospective clinical investigation. SETTING:Adult intensive care unit of a tertiary referral cancer center. PATIENTS:All head a...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-199901000-00033

    authors: Downey RJ,Friedlander P,Groeger J,Kraus D,Schantz S,Spiro R,Strong E,Shaha A,Shah J

    更新日期:1999-01-01 00:00:00

  • Prevalence and Impact of Hyponatremia in Patients With Coronavirus Disease 2019 in New York City.

    abstract:OBJECTIVES:Hyponatremia occurs in up to 30% of patients with pneumonia and is associated with increased morbidity and mortality. The prevalence of hyponatremia associated with coronavirus disease 2019 and the impact on outcome is unknown. We aimed to identify the prevalence, predictors, and impact on outcome of mild, m...

    journal_title:Critical care medicine

    pub_type: 杂志文章,多中心研究

    doi:10.1097/CCM.0000000000004605

    authors: Frontera JA,Valdes E,Huang J,Lewis A,Lord AS,Zhou T,Kahn DE,Melmed K,Czeisler BM,Yaghi S,Scher E,Wisniewski T,Balcer L,Hammer E

    更新日期:2020-12-01 00:00:00

  • Respiratory and cardiac function in children after acute hypoxemic respiratory failure.

    abstract:OBJECTIVE:To examine the pulmonary and cardiac function of children who survived an episode of acute hypoxemic respiratory failure. DESIGN:Descriptive cohort analysis. SETTING:Pediatric clinical research center of a university hospital. PATIENTS:Utilizing the criteria of PaO2 < 75 torr (< 10 kPa) with an FIO2 of > 0...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-199601000-00024

    authors: Weiss I,Ushay HM,DeBruin W,O'Loughlin J,Rosner I,Notterman D

    更新日期:1996-01-01 00:00:00

  • A simple method for diagnosing pneumonia in intubated or tracheostomized patients.

    abstract::A safe, simple, reliable technique for sampling uncontaminated peripheral bronchial secretions in patients with endotracheal tubes has been developed. It is easily performed and requires no special training or equipment. This report summarizes the techniques's usefulness in providing diagnostic samples of peripheral a...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-197703000-00003

    authors: Matthew EB,Holstrom FM,Kaspar RL

    更新日期:1977-03-01 00:00:00

  • Trends and Outcomes in Sepsis Hospitalizations With and Without Atrial Fibrillation: A Nationwide Inpatient Analysis.

    abstract:OBJECTIVES:Atrial fibrillation is frequently seen in sepsis-related hospitalizations. However, large-scale contemporary data from the United States comparing outcomes among sepsis-related hospitalizations with versus without atrial fibrillation are limited. The aim of our study was to assess the frequency of atrial fib...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/CCM.0000000000003806

    authors: Desai R,Hanna B,Singh S,Omar A,Deshmukh A,Kumar G,Foreman MG,Sachdeva R

    更新日期:2019-08-01 00:00:00

  • Granulocyte colony-stimulating factor improves survival rate and reduces concentrations of bacteria, endotoxin, tumor necrosis factor, and endothelin-1 in fulminant intra-abdominal sepsis in rats.

    abstract:OBJECTIVE:To study the therapeutic effect of granulocyte colony-stimulating factor (G-CSF) on the mortality rate and host defense pattern in fulminant intra-abdominal sepsis. DESIGN:Prospective, randomized, controlled trial. SETTING:Research laboratory in a university hospital. SUBJECTS:Adult male Wistar rats. INTE...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-199605000-00016

    authors: Lundblad R,Nesland JM,Giercksky KE

    更新日期:1996-05-01 00:00:00

  • Incidence rate of community-acquired sepsis among hospitalized acute medical patients-a population-based survey.

    abstract:OBJECTIVE:Sepsis is a frequent cause of admission, but incidence rates based on administrative data have previously produced large differences in estimates. The aim of the study was to estimate the incidence of community-acquired sepsis based on patients' symptoms and clinical findings at arrival to the hospital. DESI...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/CCM.0000000000000611

    authors: Henriksen DP,Laursen CB,Jensen TG,Hallas J,Pedersen C,Lassen AT

    更新日期:2015-01-01 00:00:00

  • Exacerbation of intestinal permeability in rats after a two-hit injury: burn and Enterococcus faecalis infection.

    abstract:OBJECTIVE:To determine alterations in intestinal epithelial permeability to solutes in burn injured rats with and without Enterococcus faecalis infection and the role of neutrophils in the intestinal permeability changes. DESIGN:Prospective sham-controlled animal study. SETTING:University research laboratory. SUBJEC...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/01.ccm.0000145579.66001.05

    authors: Samonte VA,Goto M,Ravindranath TM,Fazal N,Holloway VM,Goyal A,Gamelli RL,Sayeed MM

    更新日期:2004-11-01 00:00:00

  • Facing the challenge: decreasing case fatality rates in severe sepsis despite increasing hospitalizations.

    abstract:OBJECTIVE:To determine recent trends in severe sepsis-related rates of hospitalization, mortality, and hospital case fatality in a large geographic area and to determine the impact of age, race, and gender on these outcomes. DESIGN:Trend analysis for the period of 1995 to 2002. SETTING:Acute care hospitals in New Jer...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/01.ccm.0000186748.64438.7b

    authors: Dombrovskiy VY,Martin AA,Sunderram J,Paz HL

    更新日期:2005-11-01 00:00:00

  • Sleep and Work in ICU Physicians During a Randomized Trial of Nighttime Intensivist Staffing.

    abstract:OBJECTIVES:To compare sleep, work hours, and behavioral alertness in faculty and fellows during a randomized trial of nighttime in-hospital intensivist staffing compared with a standard daytime intensivist model. DESIGN:Prospective observational study. SETTING:Medical ICU of a tertiary care academic medical center du...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/CCM.0000000000003773

    authors: Bakhru RN,Basner M,Kerlin MP,Halpern SD,Hansen-Flaschen J,Rosen IM,Dinges DF,Schweickert WD

    更新日期:2019-07-01 00:00:00

  • Clinical validation of a new metabolic monitor suitable for use in critically ill patients.

    abstract::This report documents the validity of clinical measurements of oxygen consumption (VO2) and carbon dioxide production (VCO2) made with a new metabolic gas monitor (MGM) suitable for use in critically ill patients receiving mechanical ventilatory support. Paired samples of inspired and expired gases were obtained, and ...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-198710000-00012

    authors: Nelson LD,Anderson HB,Garcia H

    更新日期:1987-10-01 00:00:00

  • Nosocomial Infection.

    abstract:OBJECTIVE:The first 70 years of critical care can be considered a period of "industrial revolution-like" advancement in terms of progressing the understanding and care of critical illness. Unfortunately, like the industrial revolution's impact on the environment, advancing ICU care of increasingly elderly, immunosuppre...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/CCM.0000000000004783

    authors: Kollef MH,Torres A,Shorr AF,Martin-Loeches I,Micek ST

    更新日期:2021-02-01 00:00:00

  • Preventable adverse drug events in hospitalized patients: a comparative study of intensive care and general care units.

    abstract:OBJECTIVES:To compare the frequency and preventability of adverse drug events and potential adverse drug events in intensive care units (ICUs) and non-ICUs. To evaluate systems factors involving the individual caregivers, care unit teams, and patients involved in each adverse drug event by comparing ICUs with non-ICUs ...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-199708000-00014

    authors: Cullen DJ,Sweitzer BJ,Bates DW,Burdick E,Edmondson A,Leape LL

    更新日期:1997-08-01 00:00:00

  • Mechanical ventilation in fiberoptic-bronchoscopy: comparison between high frequency positive pressure ventilation and normal frequency positive pressure ventilation.

    abstract::High frequency positive pressure ventilation (HFPPV) was compared with normal frequency positive pressure ventilation (NFPPV) during diagnostic fiberoptic-bronchoscopy. HFPPV was achieved by a simple modification of the Minivent, and gave satisfactory alveolar ventilation and oxygenation. In all 11 patients and over p...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:

    authors: Flatau E,Lewinsohn G,Konichezky S,Lev A,Barzilay E

    更新日期:1982-11-01 00:00:00

  • Dimensions and Role-Specific Mediators of Surrogate Trust in the ICU.

    abstract:OBJECTIVE:In the ICU, discussions between clinicians and surrogate decision makers are often accompanied by conflict about a patient's prognosis or care plan. Trust plays a role in limiting conflict, but little is known about the determinants of trust in the ICU. We sought to identify the dimensions of trust and clinic...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/CCM.0000000000001957

    authors: Hutchison PJ,McLaughlin K,Corbridge T,Michelson KN,Emanuel L,Sporn PH,Crowley-Matoka M

    更新日期:2016-12-01 00:00:00

  • Pressure-flow relationships of the pulmonary circulation during endotoxin infusion in intact dogs.

    abstract:BACKGROUND AND METHODS:We aimed to characterize the effects of an endotoxin insult (Escherichia coli 0127:B8) on the relationships between pulmonary vascular pressure and flow in intact dogs. To achieve this goal, multipoint plots of total pressure gradient, arterial pressure gradient, and venous pressure gradient vs. ...

    journal_title:Critical care medicine

    pub_type: 杂志文章

    doi:10.1097/00003246-199207000-00018

    authors: D'Orio V,Fatemi M,Marnette JM,Mendes P,Saad G,Martinez C,Marcelle R

    更新日期:1992-07-01 00:00:00