Genomic Analysis of Microbulbifer sp. Strain A4B-17 and the Characterization of Its Metabolic Pathways for 4-Hydroxybenzoic Acid Synthesis.

Abstract:

:The marine bacterium Microbulbifer sp. A4B-17 produces secondary metabolites such as 4-hydroxybenzoic acid (4HBA) and esters of 4HBA (parabens). 4HBA is a useful material in the synthesis of the liquid crystal. Parabens are man-made compounds that have been extensively used since the 1920s in the cosmetic, pharmaceutical, and food industries for their effective antimicrobial activity. In this study, we completed the sequencing and annotation of the A4B-17 strain genome and found all genes for glucose utilization and 4HBA biosynthesis. Strain A4B-17 uses the Embden-Meyerhof-Parnas (EMP), hexose monophosphate (HMP), and Entner-Doudoroff (ED) pathways to utilize glucose. Other sugars such as fructose, sucrose, xylose, arabinose, galactose, mannitol, and glycerol supported cell growth and 4HBA synthesis. Reverse transcriptional analysis confirmed that the key genes involved in the glucose metabolism were functional. Paraben concentrations were proportionally increased by adding alcohols to the culture medium, indicating that strain A4B-17 synthesizes the 4HBA and the alcohols separately and an esterification reaction between them is responsible for the paraben synthesis. A gene that codes for a carboxylesterase was proposed to catalyze this reaction. The temperature and NaCl concentration for optimal growth were determined to be 35°C and 22.8 g/L.

journal_name

Front Microbiol

authors

Tian J,Zhu L,Wang W,Zhang L,Li Z,Zhao Q,Xing K,Feng Z,Peng X

doi

10.3389/fmicb.2018.03115

subject

Has Abstract

pub_date

2018-12-18 00:00:00

pages

3115

issn

1664-302X

journal_volume

9

pub_type

杂志文章
  • Corrigendum: Community Composition and Abundance of Bacterial, Archaeal, and Nitrifying Populations in Savanna Soils on Contrasting Bedrock Material in Kruger National Park, South Africa.

    abstract::[This corrects the article on p. 1638 in vol. 7, PMID: 27807431.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2016.01954

    authors: Rughöft S,Herrmann M,Lazar CS,Cesarz S,Levick SR,Trumbore SE,Küsel K

    更新日期:2016-11-29 00:00:00

  • Diversity and Activity of Alternative Nitrogenases in Sequenced Genomes and Coastal Environments.

    abstract::The nitrogenase enzyme, which catalyzes the reduction of N2 gas to NH4+, occurs as three separate isozyme that use Mo, Fe-only, or V. The majority of global nitrogen fixation is attributed to the more efficient 'canonical' Mo-nitrogenase, whereas Fe-only and V-('alternative') nitrogenases are often considered 'backup'...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00267

    authors: McRose DL,Zhang X,Kraepiel AM,Morel FM

    更新日期:2017-02-28 00:00:00

  • Peptidoglycan Recognition Protein 2 Regulates Neutrophil Recruitment Into the Lungs After Streptococcus pneumoniae Infection.

    abstract::Peptidoglycan (PGN) recognition proteins (PGLYRPs) are a highly conserved group of host defense proteins in insects and mammals that sense bacterial cell wall PGN and act bactericidally or cleave PGN by amidase function. Streptococcus (S.) pneumoniae is one of the top five killers worldwide and causes, e.g., pneumonia...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00199

    authors: Dabrowski AN,Conrad C,Behrendt U,Shrivastav A,Baal N,Wienhold SM,Hackstein H,N'Guessan PD,Aly S,Reppe K,Suttorp N,Zahlten J

    更新日期:2019-02-19 00:00:00

  • Potentiating the Heat Inactivation of Escherichia coli O157:H7 in Ground Beef Patties by Natural Antimicrobials.

    abstract::Escherichia coli O157: H7 (EHEC) is a major foodborne pathogen largely transmitted to humans through the consumption of undercooked ground beef. This study investigated the efficacy of two food-grade, plant-derived antimicrobials, namely rutin (RT), and resveratrol (RV) with or without chitosan (CH) in enhancing EHEC ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00015

    authors: Surendran Nair M,Lau P,Belskie K,Fancher S,Chen CH,Karumathil DP,Yin HB,Liu Y,Ma F,Upadhyaya I,Upadhyay A,Mancini R,Venkitanarayanan K

    更新日期:2016-02-02 00:00:00

  • Succession of Composition and Function of Soil Bacterial Communities During Key Rice Growth Stages.

    abstract::Elucidating the succession of soil microbial communities and microbial functions at key plant growth stages is a major goal of microbial ecology research. In this study, we investigated the succession of soil bacteria during four fertilizer treatments (control, NPK, NPK + pig manure, and NPK + straw) and at three cruc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00421

    authors: Wang W,Luo X,Chen Y,Ye X,Wang H,Cao Z,Ran W,Cui Z

    更新日期:2019-03-11 00:00:00

  • Characterization of saltern based Streptomyces sp. and statistical media optimization for its improved antibacterial activity.

    abstract::A moderately halotolerant Streptomyces strain, designated JAJ13 was characterized and a culture medium was statistically optimized to improve its antibacterial activity. Based on the phenotypic and molecular characteristics, strain JAJ13 was identified as a moderately halotolerant Streptomyces sp. JAJ13. Novelty of th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00753

    authors: Rajeswari P,Jose PA,Amiya R,Jebakumar SR

    更新日期:2015-01-21 00:00:00

  • Diversity and Expression of Bacterial Metacaspases in an Aquatic Ecosystem.

    abstract::Metacaspases are distant homologs of metazoan caspase proteases, implicated in stress response, and programmed cell death (PCD) in bacteria and phytoplankton. While the few previous studies on metacaspases have relied on cultured organisms and sequenced genomes, no studies have focused on metacaspases in a natural set...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01043

    authors: Asplund-Samuelsson J,Sundh J,Dupont CL,Allen AE,McCrow JP,Celepli NA,Bergman B,Ininbergs K,Ekman M

    更新日期:2016-07-06 00:00:00

  • The Potential Role of Arbuscular Mycorrhizal Fungi in the Restoration of Degraded Lands.

    abstract::Experiences worldwide reveal that degraded lands restoration projects achieve little success or fail. Hence, understanding the underlying causes and accordingly, devising appropriate restoration mechanisms is crucial. In doing so, the ever-increasing aspiration and global commitments in degraded lands restoration coul...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01095

    authors: Asmelash F,Bekele T,Birhane E

    更新日期:2016-07-26 00:00:00

  • Proteogenomic Analysis of Epibacterium Mobile BBCC367, a Relevant Marine Bacterium Isolated From the South Pacific Ocean.

    abstract::Epibacterium mobile BBCC367 is a marine bacterium that is common in coastal areas. It belongs to the Roseobacter clade, a widespread group in pelagic marine ecosystems. Species of the Roseobacter clade are regularly used as models to understand the evolution and physiological adaptability of generalist bacteria. E. mo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03125

    authors: Matallana-Surget S,Werner J,Wattiez R,Lebaron K,Intertaglia L,Regan C,Morris J,Teeling H,Ferrer M,Golyshin PN,Gerogiorgis D,Reilly SI,Lebaron P

    更新日期:2018-12-21 00:00:00

  • Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Cronobacter sakazakii Isolates from Powdered Infant Formula Collected from Chinese Retail Markets.

    abstract::Cronobacter sakazakii is an opportunistic pathogen that causes severe infections in neonates and infants through contaminated powdered infant formula (PIF). Therefore, the aim of this study was a large-scale study on determine the prevalence, molecular characterization and antibiotic susceptibility of C. sakazakii iso...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02026

    authors: Fei P,Jiang Y,Jiang Y,Yuan X,Yang T,Chen J,Wang Z,Kang H,Forsythe SJ

    更新日期:2017-10-17 00:00:00

  • Population Genomics of an Obligately Halophilic Basidiomycete Wallemia ichthyophaga.

    abstract:Background:Wallemia ichthyophaga is a highly specialized basidiomycetous fungus. It is one of the most halophilic fungi ever described, only able to grow at low water activity. This specialization is thought to explain why it is only rarely isolated from nature. Results:Genomes of 21 W. ichthyophaga strains were seque...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02019

    authors: Gostinčar C,Sun X,Zajc J,Fang C,Hou Y,Luo Y,Gunde-Cimerman N,Song Z

    更新日期:2019-09-04 00:00:00

  • Dynamics of Inorganic Nutrients in Intertidal Sediments: Porewater, Exchangeable, and Intracellular Pools.

    abstract::The study of inorganic nutrients dynamics in shallow sediments usually focuses on two main pools: porewater (PW) nutrients and exchangeable (EX) ammonium and phosphate. Recently, it has been found that microphytobenthos (MPB) and other microorganisms can accumulate large amounts of nutrients intracellularly (IC), high...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00761

    authors: Garcia-Robledo E,Bohorquez J,Corzo A,Jimenez-Arias JL,Papaspyrou S

    更新日期:2016-05-26 00:00:00

  • Evaluation of the biodegradation of Alaska North Slope oil in microcosms using the biodegradation model BIOB.

    abstract::We present the details of a numerical model, BIOB that is capable of simulating the biodegradation of oil entrapped in the sediment. The model uses Monod kinetics to simulate the growth of bacteria in the presence of nutrients and the subsequent consumption of hydrocarbons. The model was used to simulate experimental ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00212

    authors: Torlapati J,Boufadel MC

    更新日期:2014-05-14 00:00:00

  • Biology and Interaction of the Natural Occurrence of Distinct Monopartite Begomoviruses Associated With Satellites in Capsicum annum From India.

    abstract::Chili (Capsicum annuum L.) is an important vegetable and spice crop of tropical and sub-tropical regions. Chili plants showing upward leaf curling, leaf crinkling, and leaf yellowing symptoms, collected from Sikar district of Rajasthan, India, were found to be associated with begomovirus and satellite molecules. The p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.512957

    authors: Mishra M,Verma RK,Marwal A,Sharma P,Gaur RK

    更新日期:2020-10-07 00:00:00

  • Co-existence of Methanogenesis and Sulfate Reduction with Common Substrates in Sulfate-Rich Estuarine Sediments.

    abstract::The competition between sulfate reducing bacteria and methanogens over common substrates has been proposed as a critical control for methane production. In this study, we examined the co-existence of methanogenesis and sulfate reduction with shared substrates over a large range of sulfate concentrations and rates of s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00766

    authors: Sela-Adler M,Ronen Z,Herut B,Antler G,Vigderovich H,Eckert W,Sivan O

    更新日期:2017-05-05 00:00:00

  • Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice.

    abstract::Oral exposure to the heavy metal lead (Pb) causes various dysfunctions in animals. However, the influence of gut bacteria on Pb absorption, bioaccumulation, and excretion is largely unknown. In this study, we use a mouse model to investigate the relationship between gut microbiota, Pb-intolerant intestinal microbes an...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03161

    authors: Zhai Q,Qu D,Feng S,Yu Y,Yu L,Tian F,Zhao J,Zhang H,Chen W

    更新日期:2020-01-22 00:00:00

  • PBHMDA: Path-Based Human Microbe-Disease Association Prediction.

    abstract::With the advance of sequencing technology and microbiology, the microorganisms have been found to be closely related to various important human diseases. The increasing identification of human microbe-disease associations offers important insights into the underlying disease mechanism understanding from the perspectiv...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00233

    authors: Huang ZA,Chen X,Zhu Z,Liu H,Yan GY,You ZH,Wen Z

    更新日期:2017-02-22 00:00:00

  • A brief history of bacterial growth physiology.

    abstract::Arguably, microbial physiology started when Leeuwenhoek became fascinated by observing a Vorticella beating its cilia, my point being that almost any observation of microbes has a physiological component. With the advent of modern microbiology in the mid-19th century, the field became recognizably distinctive with suc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00289

    authors: Schaechter M

    更新日期:2015-04-21 00:00:00

  • Fecal Microbiota Alterations Associated With Diarrhea-Predominant Irritable Bowel Syndrome.

    abstract::Altered gut microbiota are assumed to be involved in the pathogenesis of irritable bowel syndrome (IBS). However, gut microbiota alterations reported in different studies are divergent and sometimes even contradictory. To better elucidate the relationship between altered gut microbiota and IBS, we characterized fecal ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01600

    authors: Zhuang X,Tian Z,Li L,Zeng Z,Chen M,Xiong L

    更新日期:2018-07-25 00:00:00

  • Response of Tomato Rhizosphere Bacteria to Root-Knot Nematodes, Fenamiphos and Sampling Time Shows Differential Effects on Low Level Taxa.

    abstract::A factorial taxonomic metabarcoding study was carried out to determine the effect of root-knot nematodes (Meloidogyne incognita, RKN) and the nematocide fenamiphos on the rhizosphere microbiome of tomato. Plants inoculated (or not) with RKN second-stage juveniles (J2), and treated (or not) with the nematocide, were te...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00390

    authors: Colagiero M,Rosso LC,Catalano D,Schena L,Ciancio A

    更新日期:2020-03-20 00:00:00

  • Antibacterial Potential of Jatropha curcas Synthesized Silver Nanoparticles against Food Borne Pathogens.

    abstract::The aqueous leaf extract of Jatropha curcas was used for the synthesis of silver nanoparticles (Jc-AgNps) which were further evaluated for its antibacterial potential against food borne pathogens. J. curcas leaf extract could synthesize stable silver nanoparticles (Zeta potential: -23.4 mV) with absorption band at 430...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01748

    authors: Chauhan N,Tyagi AK,Kumar P,Malik A

    更新日期:2016-11-08 00:00:00

  • High Iron-Sequestrating Bifidobacteria Inhibit Enteropathogen Growth and Adhesion to Intestinal Epithelial Cells In vitro.

    abstract::The gut microbiota plays an important role in host health, in particular by its barrier effect and competition with exogenous pathogenic bacteria. In the present study, the competition of Bifidobacterium pseudolongum PV8-2 (Bp PV8-2) and Bifidobacterium kashiwanohense PV20-2 (Bk PV20-2), isolated from anemic infant gu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01480

    authors: Vazquez-Gutierrez P,de Wouters T,Werder J,Chassard C,Lacroix C

    更新日期:2016-09-22 00:00:00

  • Alpine Snow Algae Microbiome Diversity in the Coast Range of British Columbia.

    abstract::Snow algae blooms contain bacteria, fungi, and other microscopic organisms. We surveyed 55 alpine snow algae blooms, collecting a total of 68 samples, from 12 mountains in the Coast Range of British Columbia, Canada. We used microscopy and rDNA metabarcoding to document biodiversity and query species and taxonomic ass...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01721

    authors: Yakimovich KM,Engstrom CB,Quarmby LM

    更新日期:2020-07-28 00:00:00

  • Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

    abstract:BACKGROUND:Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed po...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01046

    authors: Erban T,Klimov PB,Smrz J,Phillips TW,Nesvorna M,Kopecky J,Hubert J

    更新日期:2016-07-12 00:00:00

  • Animals devoid of pulmonary system as infection models in the study of lung bacterial pathogens.

    abstract::Biological disease models can be difficult and costly to develop and use on a routine basis. Particularly, in vivo lung infection models performed to study lung pathologies use to be laborious, demand a great time and commonly are associated with ethical issues. When infections in experimental animals are used, they n...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00038

    authors: López Hernández Y,Yero D,Pinos-Rodríguez JM,Gibert I

    更新日期:2015-02-04 00:00:00

  • Novel plant inputs influencing Ralstonia solanacearum during infection.

    abstract::Ralstonia solanacearum is a soil and water-borne pathogen that can infect a wide range of plants and cause the devastating bacterial wilt disease. To successfully colonize a host, R. solanacearum requires the type III secretion system (T3SS), which delivers bacterial effector proteins inside the plant cells. HrpG is a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00349

    authors: Zuluaga AP,Puigvert M,Valls M

    更新日期:2013-11-20 00:00:00

  • Characterizing Antimicrobial Resistant Escherichia coli and Associated Risk Factors in a Cross-Sectional Study of Pig Farms in Great Britain.

    abstract::Combatting antimicrobial resistant (AMR) using a One-Health approach is essential as various bacteria, including Escherichia coli, a common bacteria, are becoming increasingly resistant and livestock may be a reservoir. The AMR gene content of 492 E. coli, isolated from 56 pig farms across Great Britain in 2014-2015, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00861

    authors: AbuOun M,O'Connor HM,Stubberfield EJ,Nunez-Garcia J,Sayers E,Crook DW,Smith RP,Anjum MF

    更新日期:2020-05-25 00:00:00

  • Host Range and Symbiotic Effectiveness of N2O Reducing Bradyrhizobium Strains.

    abstract::Emissions of the potent greenhouse gas N2O is one of the environmental problems associated with intensive use of synthetic N fertilizers, and novel N2O mitigation strategies are needed to minimize fertilizer applications and N2O release without affecting agricultural efficiencies. Increased incorporation of legume cro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02746

    authors: Woliy K,Degefu T,Frostegård Å

    更新日期:2019-11-29 00:00:00

  • Modulation of Litter Decomposition by the Soil Microbial Food Web Under Influence of Land Use Change.

    abstract::Soil microbial communities modulate soil organic matter (SOM) dynamics by catalyzing litter decomposition. However, our understanding of how litter-derived carbon (C) flows through the microbial portion of the soil food web is far from comprehensive. This information is necessary to facilitate reliable predictions of ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02860

    authors: Heijboer A,de Ruiter PC,Bodelier PLE,Kowalchuk GA

    更新日期:2018-11-26 00:00:00

  • Aquaporin-4 Is Downregulated in the Basolateral Membrane of Ileum Epithelial Cells during Enterotoxigenic Escherichia coli-Induced Diarrhea in Mice.

    abstract::Enterotoxigenic Escherichia coli (ETEC) are opportunistic pathogens that colonize the small intestine, produce enterotoxins and induce diarrhea. Some aquaporins (AQPs), such as AQP3 and AQP8, have been reported to participate in diarrhea by decreasing cellular influx in the gastrointestinal (GI) tract. AQP4 is another...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02655

    authors: Zhang D,Yang L,Su W,Zhao Y,Ma X,Zhou H,Xu B,Zhang K,Ma H

    更新日期:2018-01-09 00:00:00