Potentiating the Heat Inactivation of Escherichia coli O157:H7 in Ground Beef Patties by Natural Antimicrobials.

Abstract:

:Escherichia coli O157: H7 (EHEC) is a major foodborne pathogen largely transmitted to humans through the consumption of undercooked ground beef. This study investigated the efficacy of two food-grade, plant-derived antimicrobials, namely rutin (RT), and resveratrol (RV) with or without chitosan (CH) in enhancing EHEC inactivation in undercooked hamburger patties. Further, the effect of aforementioned treatments on beef color and lipid oxidation was analyzed. Additionally, the deleterious effects of these antimicrobial treatments on EHEC was determined using scanning electron microscopy (SEM). Ground beef was inoculated with a five-strain mixture of EHEC (7.0 log CFU/g), followed by the addition of RT (0.05%, 0.1% w/w) or RV (0.1, 0.2% w/w) with or without CH (0.01% w/w). The meat was formed into patties (25 g) and stored at 4°C for 5 days. On days 1, 3, and 5, the patties were cooked (65°C, medium rare) and surviving EHEC was enumerated. The effect of these treatments on meat color and lipid oxidation during storage was also determined as per American Meat Science Association guidelines. The study was repeated three times with duplicate samples of each treatment. Both RT and RV enhanced the thermal destruction of EHEC, and reduced the pathogen load by at least 3 log CFU/g compared to control (P < 0.05). The combination of RT or RV with CH was found to be more effective, and reduced EHEC by 5 log CFU/g (P < 0.05). EHEC counts in uncooked patties did not decline during storage for 5 days (P > 0.05). Moreover, patties treated with RV plus CH were more color stable with higher a(∗) values (P < 0.05). SEM results revealed that heat treatment with antimicrobials (CH + RV 0.2%) resulted in complete destruction of EHEC cells and extrusion of intracellular contents. Results suggest that the aforementioned antimicrobials could be used for enhancing the thermal inactivation of EHEC in undercooked patties; however, detailed sensory studies are warranted.

journal_name

Front Microbiol

authors

Surendran Nair M,Lau P,Belskie K,Fancher S,Chen CH,Karumathil DP,Yin HB,Liu Y,Ma F,Upadhyaya I,Upadhyay A,Mancini R,Venkitanarayanan K

doi

10.3389/fmicb.2016.00015

subject

Has Abstract

pub_date

2016-02-02 00:00:00

pages

15

issn

1664-302X

journal_volume

7

pub_type

杂志文章
  • De novo Transcriptome Analysis of Rhizoctonia solani AG1 IA Strain Early Invasion in Zoysia japonica Root.

    abstract::Zoysia japonica brown spot was caused by necrotrophic fungus Rhizoctonia solani invasion, which led to severe financial loss in city lawn and golf ground maintenance. However, little was known about the molecular mechanism of R. solani pathogenicity in Z. japonica. In this study we examined early stage interaction bet...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00708

    authors: Zhu C,Ai L,Wang L,Yin P,Liu C,Li S,Zeng H

    更新日期:2016-05-18 00:00:00

  • Pig Farmers' Homes Harbor More Diverse Airborne Bacterial Communities Than Pig Stables or Suburban Homes.

    abstract::Airborne bacterial communities are subject to conditions ill-suited to microbial activity and growth. In spite of this, air is an important transfer medium for bacteria, with the bacteria in indoor air having potentially major consequences for the health of a building's occupants. A major example is the decreased dive...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00870

    authors: Vestergaard DV,Holst GJ,Basinas I,Elholm G,Schlünssen V,Linneberg A,Šantl-Temkiv T,Finster K,Sigsgaard T,Marshall IPG

    更新日期:2018-05-01 00:00:00

  • MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.

    abstract::Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, mic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00791

    authors: Singhal N,Kumar M,Kanaujia PK,Virdi JS

    更新日期:2015-08-05 00:00:00

  • Meta-Analysis of Vaginal Microbiome Data Provides New Insights Into Preterm Birth.

    abstract::Preterm birth (PTB) is defined as the birth of an infant before 37 weeks of gestational age. It is the leading cause of perinatal morbidity and mortality worldwide. In this study, we present a comprehensive meta-analysis of vaginal microbiome in PTB. We integrated raw longitudinal 16S rRNA vaginal microbiome data from...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00476

    authors: Kosti I,Lyalina S,Pollard KS,Butte AJ,Sirota M

    更新日期:2020-04-08 00:00:00

  • Antimicrobial Potential of Epiphytic Bacteria Associated With Seaweeds of Little Andaman, India.

    abstract::Seaweeds of the intertidal regions are a rich source of surface associated bacteria and are potential source of antimicrobial molecules. In the present study, 77 epiphytic isolates from eight different algae collected from Little Andaman were enumerated. On testing for their antimicrobial activities against certain pa...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00611

    authors: Karthick P,Mohanraju R

    更新日期:2018-04-04 00:00:00

  • Reduction-Induced Suppression of Electron Flow (RISE) Is Relieved by Non-ATP-Consuming Electron Flow in Synechococcus elongatus PCC 7942.

    abstract::Photosynthetic organisms oxidize P700 to suppress the production of reactive oxygen species (ROS) in photosystem I (PSI) in response to the lower efficiency of photosynthesis under high light and low CO2 conditions. Previously, we found a positive relationship between reduction of plastoquinone (PQ) pool and oxidation...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00886

    authors: Shimakawa G,Shaku K,Miyake C

    更新日期:2018-05-07 00:00:00

  • Efficient Biofilm-Based Fermentation Strategies for L-Threonine Production by Escherichia coli.

    abstract::Biofilms provide cells favorable growth conditions, which have been exploited in industrial biotechnological processes. However, industrial application of the biofilm has not yet been reported in Escherichia coli, one of the most important platform strains, though the biofilm has been extensively studied for pathogeni...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01773

    authors: Chen T,Liu N,Ren P,Xi X,Yang L,Sun W,Yu B,Ying H,Ouyang P,Liu D,Chen Y

    更新日期:2019-08-02 00:00:00

  • The distribution of thiamin and pyridoxine in the western tropical North Atlantic Amazon River plume.

    abstract::B-vitamins are recognized as essential organic growth factors for many organisms, although little is known about their abundance and distribution in marine ecosystems. Despite their metabolic functions regulating important enzymatic reactions, the methodology to directly measure different B-vitamins in aquatic environ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00025

    authors: Barada LP,Cutter L,Montoya JP,Webb EA,Capone DG,Sañudo-Wilhelmy SA

    更新日期:2013-03-07 00:00:00

  • Perspectives on Functional Red Mold Rice: Functional Ingredients, Production, and Application.

    abstract::Monacolin K (MK) is a secondary metabolite of the Monascus species that can inhibit cholesterol synthesis. Functional red mold rice (FRMR) is the fermentation product of Monascus spp., which is rich in MK. FRMR is usually employed to regulate serum cholesterol, especially for hypercholesterolemic patients who refuse s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.606959

    authors: Yanli F,Xiang Y

    更新日期:2020-11-25 00:00:00

  • Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases.

    abstract::Candida oleophila is an effective biocontrol agent used to control post-harvest diseases of fruits and vegetables. C. oleophila I-182 was the active agent used in the first-generation yeast-based commercial product, Aspire®, for post-harvest disease management. Several action modes, like competition for nutrients and ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00295

    authors: Sui Y,Wisniewski M,Droby S,Piombo E,Wu X,Yue J

    更新日期:2020-02-25 00:00:00

  • Long-Term Oil Contamination Alters the Molecular Ecological Networks of Soil Microbial Functional Genes.

    abstract::With knowledge on microbial composition and diversity, investigation of within-community interactions is a further step to elucidate microbial ecological functions, such as the biodegradation of hazardous contaminants. In this work, microbial functional molecular ecological networks were studied in both contaminated a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00060

    authors: Liang Y,Zhao H,Deng Y,Zhou J,Li G,Sun B

    更新日期:2016-02-03 00:00:00

  • Utilization of Host-Derived Glycans by Intestinal Lactobacillus and Bifidobacterium Species.

    abstract::Members of the genus Lactobacillus are commonly found at the gastrointestinal tract and other mucosal surfaces of humans. This genus includes various species with a great number of potentially probiotic bacteria. Other often-used probiotic species belong to Bifidobacterium, a genus almost exclusively associated with t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.01917

    authors: Zúñiga M,Monedero V,Yebra MJ

    更新日期:2018-08-17 00:00:00

  • Growth and Extended Survival of Escherichia coli O157:H7 in Soil Organic Matter.

    abstract::Enterohaemorrhagic Escherichia coli, such as serotype O157:H7, are a leading cause of food-associated outbreaks. While the primary reservoir is associated with cattle, plant foods have been associated as sources of human infection. E. coli is able to grow in the tissue of food plants such as spinach. While fecal conta...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00762

    authors: NandaKafle G,Christie AA,Vilain S,Brözel VS

    更新日期:2018-04-23 00:00:00

  • Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard.

    abstract::Microbial eukaryotes can play prominent roles in the Arctic marine ecosystem, but their diversity and variability is not well known in the ice-covered ecosystems. We determined the community composition of microbial eukaryotes in an Arctic under-ice spring bloom north of Svalbard using metabarcoding of DNA and RNA fro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01099

    authors: Meshram AR,Vader A,Kristiansen S,Gabrielsen TM

    更新日期:2017-06-28 00:00:00

  • Nanotechnology in Sustainable Agriculture: Recent Developments, Challenges, and Perspectives.

    abstract::Nanotechnology monitors a leading agricultural controlling process, especially by its miniature dimension. Additionally, many potential benefits such as enhancement of food quality and safety, reduction of agricultural inputs, enrichment of absorbing nanoscale nutrients from the soil, etc. allow the application of nan...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01014

    authors: Prasad R,Bhattacharyya A,Nguyen QD

    更新日期:2017-06-20 00:00:00

  • A Glacier Bacterium Produces High Yield of Cryoprotective Exopolysaccharide.

    abstract::Pseudomonas sp. BGI-2 is a psychrotrophic bacterium isolated from the ice sample collected from Batura glacier, Pakistan. This strain produces highly viscous colonies on agar media supplemented with glucose. In this study, we have optimized growth and production of exopolysaccharide (EPS) by the cold-adapted Pseudomon...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03096

    authors: Ali P,Shah AA,Hasan F,Hertkorn N,Gonsior M,Sajjad W,Chen F

    更新日期:2020-02-11 00:00:00

  • Gene polymorphisms in pattern recognition receptors and susceptibility to idiopathic recurrent vulvovaginal candidiasis.

    abstract:OBJECTIVE:Approximately 5% of women suffer from recurrent vulvovaginal candidiasis (RVVC). It has been hypothesized that genetic factors play an important role in the susceptibility to RVVC. The aim of this study was to assess the effect of genetic variants of genes encoding for pattern recognition receptors (PRRs) on ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00483

    authors: Rosentul DC,Delsing CE,Jaeger M,Plantinga TS,Oosting M,Costantini I,Venselaar H,Joosten LA,van der Meer JW,Dupont B,Kullberg BJ,Sobel JD,Netea MG

    更新日期:2014-09-23 00:00:00

  • Synthesis and Antibacterial Activity of Metal(loid) Nanostructures by Environmental Multi-Metal(loid) Resistant Bacteria and Metal(loid)-Reducing Flavoproteins.

    abstract::Microbes are suitable candidates to recover and decontaminate different environments from soluble metal ions, either via reduction or precipitation to generate insoluble, non-toxic derivatives. In general, microorganisms reduce toxic metal ions generating nanostructures (NS), which display great applicability in biote...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00959

    authors: Figueroa M,Fernandez V,Arenas-Salinas M,Ahumada D,Muñoz-Villagrán C,Cornejo F,Vargas E,Latorre M,Morales E,Vásquez C,Arenas F

    更新日期:2018-05-15 00:00:00

  • Azole-Resistant Aspergillus fumigatus Among Danish Cystic Fibrosis Patients: Increasing Prevalence and Dominance of TR34/L98H.

    abstract::Azole-resistant (azole-R) Aspergillus is an increasing challenge worldwide. Patients with cystic fibrosis (CF) are at risk of Aspergillus colonization and disease due to a favorable lung environment for microorganisms. We performed a nationwide study in 2018 of azole-non-susceptible Aspergillus in CF patients and comp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01850

    authors: Risum M,Hare RK,Gertsen JB,Kristensen L,Johansen HK,Helweg-Larsen J,Abou-Chakra N,Pressler T,Skov M,Jensen-Fangel S,Arendrup MC

    更新日期:2020-08-13 00:00:00

  • Genomic Reconstruction of Carbohydrate Utilization Capacities in Microbial-Mat Derived Consortia.

    abstract::Two nearly identical unicyanobacterial consortia (UCC) were previously isolated from benthic microbial mats that occur in a heliothermal saline lake in northern Washington State. Carbohydrates are a primary source of carbon and energy for most heterotrophic bacteria. Since CO2 is the only carbon source provided, the c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01304

    authors: Leyn SA,Maezato Y,Romine MF,Rodionov DA

    更新日期:2017-07-13 00:00:00

  • Glycan cross-feeding activities between bifidobacteria under in vitro conditions.

    abstract::Bifidobacteria colonize the gut of various mammals, including humans, where they may metabolize complex, diet-, and host-derived carbohydrates. The glycan-associated metabolic features encoded by bifidobacteria are believed to be strongly influenced by cross-feeding activities due to the co-existence of strains with d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01030

    authors: Turroni F,Özcan E,Milani C,Mancabelli L,Viappiani A,van Sinderen D,Sela DA,Ventura M

    更新日期:2015-09-24 00:00:00

  • Marine sediments microbes capable of electrode oxidation as a surrogate for lithotrophic insoluble substrate metabolism.

    abstract::Little is known about the importance and/or mechanisms of biological mineral oxidation in sediments, partially due to the difficulties associated with culturing mineral-oxidizing microbes. We demonstrate that electrochemical enrichment is a feasible approach for isolation of microbes capable of gaining electrons from ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00784

    authors: Rowe AR,Chellamuthu P,Lam B,Okamoto A,Nealson KH

    更新日期:2015-01-14 00:00:00

  • Isolation and Genomic Characterization of an Acinetobacter johnsonii Bacteriophage AJO2 From Bulking Activated Sludge.

    abstract::A novel Podoviridae lytic phage AJO2, specifically infecting Acinetobacter johnsonii, was isolated from bulking activated sludge. The one-step growth experiment showed that the latent period and burst size of AJO2 were estimated to be 30 min and 78.1 phages per infected cell, respectively. The viability test indicated...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00266

    authors: Fan N,Yang M,Jin R,Qi R

    更新日期:2019-02-20 00:00:00

  • Seasonal and Spatial Variations in Bacterial Communities From Tetrodotoxin-Bearing and Non-tetrodotoxin-Bearing Clams.

    abstract::Tetrodotoxin (TTX) is one of the most potent naturally occurring compounds and is responsible for many human intoxications worldwide. Paphies australis are endemic clams to New Zealand which contain varying concentrations of TTX. Research suggests that P. australis accumulate the toxin exogenously, but the source rema...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01860

    authors: Biessy L,Pearman JK,Smith KF,Hawes I,Wood SA

    更新日期:2020-08-05 00:00:00

  • Development of a Sterne-Based Complement Fixation Test to Monitor the Humoral Response Induced by Anthrax Vaccines.

    abstract::Anthrax is a zoonotic disease caused by Bacillus anthracis spore-forming bacterium. Since it is primarily a disease of animals, the control in animals, and humans depend on the prevention in livestock, principally cattle, sheep, and goats. Most veterinary vaccines utilize the toxigenic, uncapsulated (pXO1+/pXO2-) B. a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00019

    authors: Adone R,Sali M,Francia M,Iatarola M,Donatiello A,Fasanella A

    更新日期:2016-01-28 00:00:00

  • Effect of Organic Carbon and Nitrogen on the Interactions of Morchella spp. and Bacteria Dispersing on Their Mycelium.

    abstract::In this study we investigated how the source of organic carbon (Corg) and nitrogen (Norg) affects the interactions between fungi of the genus Morchella and bacteria dispersing along their hyphae (fungal highways; FH). We demonstrated that bacteria using FH increase the hydrolysis of an organic nitrogen source that onl...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00124

    authors: Lohberger A,Spangenberg JE,Ventura Y,Bindschedler S,Verrecchia EP,Bshary R,Junier P

    更新日期:2019-03-01 00:00:00

  • Inhibition of HTLV-1 Infection by HIV-1 First- and Second-Generation Integrase Strand Transfer Inhibitors.

    abstract::More than 10 million people worldwide are infected with the retrovirus human T-cell lymphotropic virus type 1 (HTLV-1). Infection phenotypes can range from asymptomatic to severe adult T-cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy. HTLV-1, like human immunodeficiency virus type 1 (HIV-1), is a blood...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01877

    authors: Barski MS,Minnell JJ,Maertens GN

    更新日期:2019-08-13 00:00:00

  • Alpine Snow Algae Microbiome Diversity in the Coast Range of British Columbia.

    abstract::Snow algae blooms contain bacteria, fungi, and other microscopic organisms. We surveyed 55 alpine snow algae blooms, collecting a total of 68 samples, from 12 mountains in the Coast Range of British Columbia, Canada. We used microscopy and rDNA metabarcoding to document biodiversity and query species and taxonomic ass...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01721

    authors: Yakimovich KM,Engstrom CB,Quarmby LM

    更新日期:2020-07-28 00:00:00

  • NprR-NprX Quorum-Sensing System Regulates the Algicidal Activity of Bacillus sp. Strain S51107 against Bloom-Forming Cyanobacterium Microcystis aeruginosa.

    abstract::Harmful cyanobacterial blooms have severely impaired freshwater quality and threatened human health worldwide. Here, a Gram-positive bacterium, Bacillus sp. strain S51107, which exhibits strong algicidal activity against Microcystis aeruginosa, was isolated from Lake Taihu. We found that the algicidal activity of stra...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01968

    authors: Wu L,Guo X,Liu X,Yang H

    更新日期:2017-10-11 00:00:00

  • Microbial Communities in Sediments From Four Mildly Acidic Ephemeral Salt Lakes in the Yilgarn Craton (Australia) - Terrestrial Analogs to Ancient Mars.

    abstract::The Yilgarn Craton in Australia has a large number of naturally occurring shallow ephemeral lakes underlain by a dendritic system of paleodrainage channels. Processes like evaporation, flooding, erosion, as well as inflow of saline, often acidic and ion-rich groundwater contribute to the (dynamic) nature of the lakes ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00779

    authors: Aerts JW,van Spanning RJM,Flahaut J,Molenaar D,Bland PA,Genge MJ,Ehrenfreund P,Martins Z

    更新日期:2019-05-06 00:00:00