Hydrogels Embedded With Melittin and Tobramycin Are Effective Against Pseudomonas aeruginosa Biofilms in an Animal Wound Model.

Abstract:

:We demonstrate that the antimicrobial peptide, melittin, is effective alone and in combination with the aminoglycosides tobramycin to kill Pseudomonas aeruginosa growing as biofilms both in vitro and in vivo. Melittin and tobramycin show enhanced in vitro activity in combination at micromolar concentrations, resulting in a 2-log10 reduction in the number of cells within mature PAO1 P. aeruginosa biofilms after 6-h of treatment. Alternatively, either agent alone resulted in half-a-log10 reduction. Time-killing assays demonstrated that the combination of melittin and tobramycin was effective at 2-h whereas tobramycin was not effective until after 6-h of treatment. We also found the combination was more effective than tobramycin alone against biofilms of 7 P. aeruginosa cystic fibrosis clinical isolates, resulting in a maximum 1.5-log10 cellular reduction. Additionally, melittin alone was effective at killing biofilms of 4 Staphylococcus aureus isolates, resulting in a maximum 2-log10 cellular reduction. Finally, melittin in combination with tobramycin embedded in an agarose-based hydrogel resulted in a 4-fold reduction in bioluminescent P. aeruginosa colonizing mouse wounds by 4-h. In contrast, tobramycin or melittin treatment alone did not cause a statistically significant reduction in bioluminescence. These data demonstrate that melittin in combination with tobramycin embedded in a hydrogel is a potential treatment for biofilm-associated wound infections.

journal_name

Front Microbiol

authors

Maiden MM,Zachos MP,Waters CM

doi

10.3389/fmicb.2019.01348

subject

Has Abstract

pub_date

2019-06-20 00:00:00

pages

1348

issn

1664-302X

journal_volume

10

pub_type

杂志文章
  • A Disulfide Bond in the Membrane Protein IgaA Is Essential for Repression of the RcsCDB System.

    abstract::IgaA is an integral inner membrane protein that was discovered as repressor of the RcsCDB phosphorelay system in the intracellular pathogen Salmonella enterica serovar Typhimurium. The RcsCDB system, conserved in many members of the family Enterobacteriaceae, regulates expression of varied processes including motility...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02605

    authors: Pucciarelli MG,Rodríguez L,García-Del Portillo F

    更新日期:2017-12-22 00:00:00

  • Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues.

    abstract::Zika virus (ZIKV) infection in humans has been associated with congenital malformations and other neurological disorders, such as Guillain-Barré syndrome. The mechanism(s) of ZIKV intrauterine transmission, the cell types involved, the most vulnerable period of pregnancy for severe outcomes from infection and other ph...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02266

    authors: de Noronha L,Zanluca C,Burger M,Suzukawa AA,Azevedo M,Rebutini PZ,Novadzki IM,Tanabe LS,Presibella MM,Duarte Dos Santos CN

    更新日期:2018-09-25 00:00:00

  • Staphylococcal Protein A Promotes Colonization and Immune Evasion of the Epidemic Healthcare-Associated MRSA ST239.

    abstract::The highly successful epidemic of healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) ST239 is a growing concern worldwide, due to its progressive adaptation to the highly selective environment of the healthcare system. HA-MRSA ST239 display the reduced virulence and successfully colonize in ho...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00951

    authors: Hong X,Qin J,Li T,Dai Y,Wang Y,Liu Q,He L,Lu H,Gao Q,Lin Y,Li M

    更新日期:2016-06-27 00:00:00

  • Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional Profiles in an Oil Production Facility.

    abstract::DNA and RNA-based sequencing of the 16S rRNA gene and transcripts were used to assess the phylogenetic diversity of microbial communities at assets experiencing corrosion in an oil production facility. The complementary methodological approach, coupled with extensive bioinformatics analysis, allowed to visualize diffe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02587

    authors: Salgar-Chaparro SJ,Machuca LL

    更新日期:2019-11-07 00:00:00

  • MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats.

    abstract::Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF MS) has been used routinely over the past decade in clinical microbiology laboratories to rapidly characterize diverse microorganisms of medical importance both at the genus and species levels. Currently, there is keen interest in ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02442

    authors: Tuohy JM,Mueller-Spitz SR,Albert CM,Scholz-Ng SE,Wall ME,Noutsios GT,Gutierrez AJ,Sandrin TR

    更新日期:2018-10-15 00:00:00

  • Characterization of KPC-Producing Serratia marcescens in an Intensive Care Unit of a Brazilian Tertiary Hospital.

    abstract::Serratia marcescens has emerged as an important opportunistic pathogen responsible for nosocomial and severe infections. Here, we determined phenotypic and molecular characteristics of 54 S. marcescens isolates obtained from patient samples from intensive-care-unit (ICU) and neonatal intensive-care-unit (NIUC) of a Br...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00956

    authors: Ferreira RL,Rezende GS,Damas MSF,Oliveira-Silva M,Pitondo-Silva A,Brito MCA,Leonardecz E,de Góes FR,Campanini EB,Malavazi I,da Cunha AF,Pranchevicius MDS

    更新日期:2020-05-20 00:00:00

  • Antimicrobial and Synergistic Activity of 2,2',4-Trihydroxybenzophenone Against Bacterial Pathogens of Poultry.

    abstract::In poultry farming, the spread of bacterial pathogens results in disease outbreaks causing significant economic losses to this industry. Many of these pathogenic bacteria are zoonotic and have a substantial impact on public health. Antimicrobials are essential for the prevention and treatment of these bacterial infect...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00490

    authors: Aranda MIR,Gómez GAT,de Barros M,Dos Santos MH,de Oliveira LL,Pena JL,Moreira MAS

    更新日期:2019-03-20 00:00:00

  • Light-driven increase in carbon yield is linked to maintenance in the proteorhodopsin-containing Photobacterium angustum S14.

    abstract::A type of photoheterotrophic bacteria contain a transmembrane light-driven proton pump called proteorhodopsins (PRs). Due to the prevalence of these organisms in the upper water column of the World's Ocean, and their potential for light-driven ATP generation, they have been suggested to significantly influence energy ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00688

    authors: Courties A,Riedel T,Rapaport A,Lebaron P,Suzuki MT

    更新日期:2015-07-10 00:00:00

  • Antifungal Streptomyces spp. Associated with the Infructescences of Protea spp. in South Africa.

    abstract::Common saprophytic fungi are seldom present in Protea infructescences, which is strange given the abundance of mainly dead plant tissue in this moist protected environment. We hypothesized that the absence of common saprophytic fungi in Protea infructescences could be due to a special symbiosis where the presence of m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01657

    authors: Human ZR,Moon K,Bae M,de Beer ZW,Cha S,Wingfield MJ,Slippers B,Oh DC,Venter SN

    更新日期:2016-11-02 00:00:00

  • Dominance Between Plasmids Determines the Extent of Biofilm Formation.

    abstract::Bacterial biofilms have an impact in medical and industrial environments because they often confer protection to bacteria against harmful agents, and constitute a source from which microorganisms can disperse. Conjugative plasmids can enhance bacterial ability to form biofilms because conjugative pili act as adhesion ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.02070

    authors: Gama JA,Fredheim EGA,Cléon F,Reis AM,Zilhão R,Dionisio F

    更新日期:2020-08-26 00:00:00

  • Pathobiomes Differ between Two Diseases Affecting Reef Building Coralline Algae.

    abstract::Crustose coralline algae (CCA) are major benthic calcifiers that play crucial roles in coral reef ecosystems. Two diseases affecting CCA have recently been investigated: coralline white band syndrome (CWBS) and coralline white patch disease (CWPD). These diseases can trigger major losses in CCA cover on tropical coral...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01686

    authors: Meistertzheim AL,Nugues MM,Quéré G,Galand PE

    更新日期:2017-09-01 00:00:00

  • Rare Plankton Subcommunities Are Far More Affected by DNA Extraction Kits Than Abundant Plankton.

    abstract::Advances in high-throughput sequencing technologies allow a more complete study of microbial plankton community composition and diversity, especially in the rare microbial biosphere. The DNA extraction of plankton is a key step for such studies; however, little is known about its influences on the abundant or rare mic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00454

    authors: Liu M,Xue Y,Yang J

    更新日期:2019-03-11 00:00:00

  • Genomic Characterization of Sulphite Reducing Bacteria Isolated From the Dairy Production Chain.

    abstract::Anaerobic sporeformers, specifically spoilage and pathogenic members of the genus Clostridium, are a concern for producers of dairy products, and of powdered dairy products in particular. As an alternative to testing for individual species, the traditional, and still current, approach to detecting these sporeformers, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01507

    authors: Doyle CJ,O'Toole PW,Cotter PD

    更新日期:2018-07-05 00:00:00

  • Characterization of IncC Plasmids in Enterobacterales of Food-Producing Animals Originating From China.

    abstract::Incompatibility group C (IncC) plasmids have received attention due to their broad host range and because they harbor key antibiotic resistance genes. Because these resistance genes can spread from food-producing animals to human, the proliferation of these plasmids represents a public health risk. In this study, a to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.580960

    authors: Zhang Y,Lei CW,Chen X,Yao TG,Yu JW,Hu WL,Mao X,Wang HN

    更新日期:2020-10-27 00:00:00

  • Phylodynamic analysis of a viral infection network.

    abstract::Viral infections by sexual and droplet transmission routes typically spread through a complex host-to-host contact network. Clarifying the transmission network and epidemiological parameters affecting the variations and dynamics of a specific pathogen is a major issue in the control of infectious diseases. However, co...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00278

    authors: Shiino T

    更新日期:2012-07-31 00:00:00

  • Recovering Genomics Clusters of Secondary Metabolites from Lakes Using Genome-Resolved Metagenomics.

    abstract::Metagenomic approaches became increasingly popular in the past decades due to decreasing costs of DNA sequencing and bioinformatics development. So far, however, the recovery of long genes coding for secondary metabolites still represents a big challenge. Often, the quality of metagenome assemblies is poor, especially...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00251

    authors: Cuadrat RRC,Ionescu D,Dávila AMR,Grossart HP

    更新日期:2018-02-20 00:00:00

  • Complexity of Human Antibody Response to Dengue Virus: Implication for Vaccine Development.

    abstract::The four serotypes of dengue virus (DENV) are the leading cause of arboviral diseases in humans. Decades of efforts have made remarkable progress in dengue vaccine development. Despite the first dengue vaccine (dengvaxia from Sanofi Pasteur), a live-attenuated tetravalent chimeric yellow fever-dengue vaccine, has been...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01372

    authors: Tsai WY,Lin HE,Wang WK

    更新日期:2017-07-20 00:00:00

  • A Mosaic of Geothermal and Marine Features Shapes Microbial Community Structure on Deception Island Volcano, Antarctica.

    abstract::Active volcanoes in Antarctica contrast with their predominantly cold surroundings, resulting in environmental conditions capable of selecting for versatile and extremely diverse microbial communities. This is especially true on Deception Island, where geothermal, marine, and polar environments combine to create an ex...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00899

    authors: Bendia AG,Signori CN,Franco DC,Duarte RTD,Bohannan BJM,Pellizari VH

    更新日期:2018-05-07 00:00:00

  • The Stress-Responsive Alternative Sigma Factor SigB of Bacillus subtilis and Its Relatives: An Old Friend With New Functions.

    abstract::Alternative sigma factors have led the core RNA polymerase (RNAP) to recognize different sets of promoters to those recognized by the housekeeping sigma A-directed RNAP. This change in RNAP promoter selectivity allows a rapid and flexible reformulation of the genetic program to face environmental and metabolic stimuli...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01761

    authors: Rodriguez Ayala F,Bartolini M,Grau R

    更新日期:2020-09-15 00:00:00

  • Microbiome-Guided Exploration of the Microbial Assemblage of the Exotic Beverage "Insect Tea" Native to Southwestern China.

    abstract::Insect tea is a unique beverage that is native to Southwestern China and traditionally produced by local farmers in an elaborate process. It consists of insect larvae excrements that are commonly obtained from meal moths (Pyralis farinalis Linnaeus 1758) reared on a specific plant-based diet. We have reconstructed the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03087

    authors: Mao X,Kusstatscher P,Li H,Chen X,Berg G,Yang M,Cernava T

    更新日期:2020-01-29 00:00:00

  • State-Wide Genomic and Epidemiological Analyses of Vancomycin-Resistant Enterococcus faecium in Tasmania's Public Hospitals.

    abstract::From 2015 onwards, the number of vancomycin-resistant Enterococcus faecium (VREfm) isolates increased in Tasmania. Previously, we examined the transmission of VREfm at the Royal Hobart Hospital (RHH). In this study, we performed a state-wide analysis of VREfm from Tasmania's four public acute hospitals. Whole-genome a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02940

    authors: Leong KWC,Kalukottege R,Cooley LA,Anderson TL,Wells A,Langford E,O'Toole RF

    更新日期:2020-01-15 00:00:00

  • The Biogeochemical Sulfur Cycle of Marine Sediments.

    abstract::Microbial dissimilatory sulfate reduction to sulfide is a predominant terminal pathway of organic matter mineralization in the anoxic seabed. Chemical or microbial oxidation of the produced sulfide establishes a complex network of pathways in the sulfur cycle, leading to intermediate sulfur species and partly back to ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.00849

    authors: Jørgensen BB,Findlay AJ,Pellerin A

    更新日期:2019-04-24 00:00:00

  • Cytometric fingerprints: evaluation of new tools for analyzing microbial community dynamics.

    abstract::Optical characteristics of individual bacterial cells of natural communities can be measured with flow cytometry (FCM) in high throughput. The resulting data are visualized in cytometric histograms. These histograms represent individual cytometric fingerprints of microbial communities, e.g., at certain time points or ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00273

    authors: Koch C,Harnisch F,Schröder U,Müller S

    更新日期:2014-06-04 00:00:00

  • Assembly and Succession of Iron Oxide Microbial Mat Communities in Acidic Geothermal Springs.

    abstract::Biomineralized ferric oxide microbial mats are ubiquitous features on Earth, are common in hot springs of Yellowstone National Park (YNP, WY, USA), and form due to direct interaction between microbial and physicochemical processes. The overall goal of this study was to determine the contribution of different community...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00025

    authors: Beam JP,Bernstein HC,Jay ZJ,Kozubal MA,Jennings Rd,Tringe SG,Inskeep WP

    更新日期:2016-02-15 00:00:00

  • PipY, a Member of the Conserved COG0325 Family of PLP-Binding Proteins, Expands the Cyanobacterial Nitrogen Regulatory Network.

    abstract::Synechococcus elongatus PCC 7942 is a paradigmatic model organism for nitrogen regulation in cyanobacteria. Expression of genes involved in nitrogen assimilation is positively regulated by the 2-oxoglutarate receptor and global transcriptional regulator NtcA. Maximal activation requires the subsequent binding of the c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01244

    authors: Labella JI,Cantos R,Espinosa J,Forcada-Nadal A,Rubio V,Contreras A

    更新日期:2017-07-11 00:00:00

  • Vibrational Spectroscopy for Imaging Single Microbial Cells in Complex Biological Samples.

    abstract::Vibrational spectroscopy is increasingly used for the rapid and non-destructive imaging of environmental and medical samples. Both Raman and Fourier-transform infrared (FT-IR) imaging have been applied to obtain detailed information on the chemical composition of biological materials, ranging from single microbial cel...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00675

    authors: Harrison JP,Berry D

    更新日期:2017-04-13 00:00:00

  • GLAPD: Whole Genome Based LAMP Primer Design for a Set of Target Genomes.

    abstract::Loop-mediated isothermal amplification (LAMP) technology has been applied in a wide range of fields such as detection of foodborne bacteria and clinical pathogens due to its simplicity and efficiency. However, existing LAMP primer designing systems require a conserved gene or a short genome region as input, and they c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02860

    authors: Jia B,Li X,Liu W,Lu C,Lu X,Ma L,Li YY,Wei C

    更新日期:2019-12-13 00:00:00

  • The Developmental Stage Symbionts of the Pea Aphid-Feeding Chrysoperla sinica (Tjeder).

    abstract::Chrysoperla sinica (Tjeder) is widely recognized as an important holometabolous natural enemy of various insect pests in different cropping systems and as a non-target surrogate in environmental risk assessment of Bt rice (i.e., genetically modified rice to express a toxin gene from Bacillus thuringiensis). Like other...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02454

    authors: Zhao C,Zhao H,Zhang S,Luo J,Zhu X,Wang L,Zhao P,Hua H,Cui J

    更新日期:2019-11-01 00:00:00

  • An RNA Virome Associated to the Golden Orb-Weaver Spider Nephila clavipes.

    abstract::The golden orb-weaver spider Nephila clavipes, known for its sexual size dimorphism, is abundant and widespread in the New World. The first annotated genome of orb-weaver spiders, exploring N. clavipes, has recently been reported. The study, focused primarily on the diversity of silk specific genes, shed light into th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02097

    authors: Debat HJ

    更新日期:2017-10-25 00:00:00

  • A Replisome's journey through the bacterial chromosome.

    abstract::Genome duplication requires the coordinated activity of a multi-component machine, the replisome. In contrast to the background of metabolic diversity across the bacterial domain, the composition and architecture of the bacterial replisome seem to have suffered few changes during evolution. This immutability underline...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00562

    authors: Beattie TR,Reyes-Lamothe R

    更新日期:2015-06-05 00:00:00