Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever.

Abstract:

:Historically, disease progression in animal models of Q fever has been carried out using PCR to monitor the presence of Coxiella burnetii DNA in the host. However, the colonization and dissemination of other bacterial infections in animal models are tracked using viable counts, enabling an accurate assessment of viable bacterial load within tissues. Following recent advances in the culture methods, it has become possible to do the same with C. burnetii. Here we compare and contrast the different information gained by using PCR or viable counts to study this disease. Viable bacteria were cleared from organs much faster than previously reported when assessed by bacterial DNA, but weight loss and clinical signs improved while animals were still heavily infected.

journal_name

Front Microbiol

authors

Hartley MG,Ralph E,Norville IH,Prior JL,Atkins TP

doi

10.3389/fmicb.2019.01552

subject

Has Abstract

pub_date

2019-07-16 00:00:00

pages

1552

issn

1664-302X

journal_volume

10

pub_type

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