Abstract:
:There is no use restriction associated with bees for many fungicides used in agriculture; however, this does not always mean that these pesticides are harmless for these nontarget organisms. We investigated whether the fungicide pyraclostrobin, which acts on fungal mitochondria, also negatively affects honey bee mitochondrial bioenergetics. Honey bees were collected from 5 hives and anesthetized at 4 °C. The thoraces were separated, and mitochondria were isolated by grinding, filtering, and differential centrifugation. An aliquot of 0.5 mg of mitochondrial proteins was added to 0.5 mL of a standard reaction medium with 4 mM succinate (complex II substrate) plus 50 nM rotenone (complex I inhibitor), and mitochondrial respiration was measured at 30 °C using a Clark-type oxygen electrode. Mitochondrial membrane potential was determined spectrofluorimetrically using safranin O as a probe, and adenosine triphosphate (ATP) synthesis was determined by chemiluminescence. Pyraclostrobin at 0 to 50 μM was tested on the mitochondrial preparations, with 3 repetitions. Pyraclostrobin inhibited mitochondrial respiration in a dose-dependent manner at concentrations of 10 μM and above, demonstrating typical inhibition of oxidative phosphorylation. Pyraclostrobin also promoted a decline in the mitochondrial membrane potential at doses of 5 μM and above and in ATP synthesis at 15 μM and above. We conclude that pyraclostrobin interferes with honey bee mitochondrial function, which is especially critical for the energy-demanding flight activity of foraging bees. Environ Toxicol Chem 2020;39:1267-1272. © 2020 SETAC.
journal_name
Environ Toxicol Chemjournal_title
Environmental toxicology and chemistryauthors
Nicodemo D,Mingatto FE,De Jong D,Bizerra PFV,Tavares MA,Bellini WC,Vicente EF,de Carvalho Adoi
10.1002/etc.4719subject
Has Abstractpub_date
2020-05-01 00:00:00pages
1267-1272issue
6eissn
0730-7268issn
1552-8618journal_volume
39pub_type
杂志文章abstract::We examined the effects of multigenerational exposure of mercury (Hg) on Hg toxicity and biokinetics in a population of Daphnia magna. After chronic Hg exposure at 3.8 microg Hg/L, the first generation (F0) adults had an elevated 24-h median lethal concentration (LC50) of Hg (76 microg/L) when compared to the control ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-085r.1
更新日期:2005-11-01 00:00:00
abstract::Antibiotics are used to maintain healthy livestock and to promote weight gain in concentrated animal feed operations. Antibiotics rarely are metabolized completely by livestock and, thus, are often present in livestock waste and in waste-treatment lagoons. The introduction of antibiotics into anaerobic lagoons commonl...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/04-093r.1
更新日期:2005-04-01 00:00:00
abstract::Aerobic soil metabolism of uniconazole-P ([S]-E-1-[4-chlorophenyl]-4,4-dimethyl-2-[1,2,4-triazole-1-yl]-penten-3-ol) and the effect of illumination on metabolic profiles were studied in the water-sediment system when spiked to water. Uniconazole-P was gradually partitioned to the sediment with an aquatic half-life of ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-116ra.1
更新日期:2006-02-01 00:00:00
abstract::The present study investigated the effects of 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE) on the thyroid and steroid-metabolizing system in Atlantic salmon (Salmo salar) parr. Fish were exposed to waterborne DDE and thyroxine (T4), both singly and in combination, for 5 d. Thyroid-stimulating hormone (TSHbeta), ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-376r1.1
更新日期:2006-06-01 00:00:00
abstract::The effect of major cation activity (Ca(2+) , Mg(2+) , Na(+) , K(+) ) on Ni toxicity, with dose expressed as exposure (total dissolved Ni concentration NiTot ) or free Ni ion activity (in solution Ni(2+) ), or as tissue residue (Ni concentration in plant tissue NiTiss ) to the aquatic plant Lemna minor L. was examined...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.2116
更新日期:2013-04-01 00:00:00
abstract::Given the urgent need for ecologically relevant toxicity tools specific to estuarine systems, the main goal of the present study was to develop and evaluate the potential of an in situ algal assay for estuarine sediment-overlying water toxicity evaluations using the microalga Phaeodactylum tricornutum immobilized in a...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-266r.1
更新日期:2006-09-01 00:00:00
abstract::The radiotracer technique was used to assess the influences of sediment-metal contact time or aging (up to two years) on the bioavailability and geochemical speciation of Cd and Zn in sediments. Bioavailability was quantified by measuring the assimilation efficiency of metals in two deposit-feeding invertebrates (sipu...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/06-166.1
更新日期:2006-10-01 00:00:00
abstract::The present study evaluated if the element profiles in hair and nails of children from the Lake Victoria region in Kenya reflect uptake from food and the environment. To this purpose, the element concentrations in hair and nails were related to element concentrations measured in food items, water, and soil. The highes...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.1863
更新日期:2012-07-01 00:00:00
abstract::Amphibian population declines have been documented worldwide and environmental contaminants are believed to contribute to some declines. Maternal transfer of bioaccumulated contaminants to offspring may be an important and overlooked mechanism of impaired reproductive success that affects amphibian populations. Mercur...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.125
更新日期:2010-04-01 00:00:00
abstract::Stenotrophomonas sp. CD02 was isolated from a site that previously had been contaminated with high concentrations of the heavy metals cadmium (3 mg kg(-1)) and chromium (115 mg kg(-1)). This strain was able to grow on complex (Luria Bertani) medium containing high concentrations of cadmium ion (up to 4 mM). Additional...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/06-280r.1
更新日期:2007-04-01 00:00:00
abstract::Nitrogen, sulfur, or oxygen heterocyclic aromatic hydrocarbons (NSO-HETs) and short-chained alkyl phenols (SCAPs) are commonly detected in groundwater at contaminated sites and in the surrounding environment. It is now scientific consensus that these chemicals pose a risk to human and ecosystem health. However, toxici...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.4423
更新日期:2019-06-01 00:00:00
abstract::Extensive industrial areas in headwater stream watersheds can severely impact the physical condition of streams and introduce contaminants. We compared 3 streams that received stormwater runoff and industrial effluents from industrial complexes to 2 reference streams. Reference streams provide a benchmark of compariso...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.4287
更新日期:2019-01-01 00:00:00
abstract::Currently, environmental studies describing levels of polychlorinated biphenyls (PCBs) in imported shrimp are limited, particularly studies of aquaculture shrimp. In the present study, we measured concentrations of the 209 PCB congeners in 84 uncooked, warm-water shrimp samples from the United States and 14 other coun...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.1803
更新日期:2012-05-01 00:00:00
abstract::Heavy metals can represent a threat to the health of aquatic ecosystems. Unlike organic chemicals, heavy metals cannot be eliminated by natural processes such as their degradation into less toxic compounds, and this creates unique challenges for their remediation from soil, water, and air. Phytoremediation, defined as...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章,评审
doi:10.1002/etc.4909
更新日期:2021-01-01 00:00:00
abstract::We conducted a seasonal study of the uptake of Cd, Se(IV), and Zn by natural phytoplankton assemblages collected from two locations in Hong Kong coastal waters. Marine phytoplankton in Port Shelter was dominated by diatoms and cyanobacteria, while phytoplankton in Tolo Harbour was dominated by dinoflagellates. The nat...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/04-125r.1
更新日期:2005-01-01 00:00:00
abstract::Northern bobwhite (quail) (Colinus virginianus) and scaled quail (Callipepla squamata) populations have declined dramatically in the Rolling Plains ecoregion of Texas and Oklahoma (USA). There is rising concern about potential toxicity of neonicotinoids to birds. To investigate this concern, the authors examined crops...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.3305
更新日期:2016-06-01 00:00:00
abstract::Monte Carlo simulations were used to evaluate statistical methods for estimating 95% upper confidence limits of mean constituent concentrations for left-censored data with nonuniform detection limits. Two primary scenarios were evaluated: data sets with 15 to 50% nondetected samples and data sets with 51 to 80% nondet...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-548r.1
更新日期:2006-09-01 00:00:00
abstract::A modeling study was conducted to examine the distribution of concentrations of the antimicrobial triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether) in rivers following discharge from wastewater treatment plants (WWTPs). Most uses of triclosan are disposed of down residential drains and ultimately reach WWTPs. A mo...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract::An original species-selection method for the building of test batteries is presented. This method is based on the statistical analysis of the biological and ecological trait patterns of species. It has been applied to build a macroinvertebrate test battery for the assessment of sediment toxicity, which efficiently des...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/04-559r.1
更新日期:2005-09-01 00:00:00
abstract::Oxygen consumption is indicative of an organism's metabolic state, whereby alterations in respiration rate can result from the presence of different stimuli. Here, we develop a novel approach based on quenched fluorescence oxygen sensing and respirometry method for toxicity screening assays using the nematode Caenorha...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/08-083.1
更新日期:2009-04-01 00:00:00
abstract::Sediment-quality assessment often is hindered by the lack of agreement between chemical and biological lines of evidence. One limitation is that the bulk sediment toxicant concentration, the most widely used chemical parameter, does not always represent the bioavailable concentration, particularly for hydrophobic orga...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/08-322R.1
更新日期:2009-04-01 00:00:00
abstract::Cucurbita pepo spp pepo (zucchini) is known as an exceptional weathered dichlorodiphenyldichloroethylene (DDE) accumulator, whereas Cucurbita pepo ssp ovifera (squash) is termed a nonaccumulator. Experiments were conducted with hybridized zucchini and squash to assess the inheritance pattern of DDX (the sum of p,p'-di...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.1887
更新日期:2012-08-01 00:00:00
abstract::In metal-polluted environments, high concentrations of metals such as zinc, cadmium, copper, and lead are present. Many studies have been conducted to determine uptake and binding of the nonessential, toxic metal cadmium. Effects of essential metals on this uptake and binding remain unclear. The aim of the present stu...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::A microbial consortium that degrades ethyl-tert-butyl ether (ETBE) as the sole source of carbon and energy under aerobic conditions was selected from a gasoline-polluted soil. This consortium consists of a variety of microorganisms with a predominance of filamentous morphology. Degradation of ETBE was found to be sole...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::The availability of literature regarding sublethal and chronic toxicity of heavy metals to early life stages of marine species is restricted to a few species of invertebrate mollusks. The early life stage of abalone, an important gastropod both environmentally and commercially, has been involved in limited research in...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-060r.1
更新日期:2006-05-01 00:00:00
abstract::We conducted acute lethality tests with white sturgeon (Acipenser transmontanus) and Ceriodaphnia dubia exposed to copper and zinc at dissolved organic carbon concentrations ranging from 0.5 to 5.5 mg/L. Dissolved organic carbon had minimal effects on zinc toxicity but did have a protective effect on acute copper toxi...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.4592
更新日期:2019-12-01 00:00:00
abstract::Perfluoro alkyl acids (PFAAs) are known to bioconcentrate in plants grown in contaminated soils; the potential risk from consuming these plants is currently less understood. We determined that the current daily reference doses (RfDs) of the US Environmental Protection Agency (USEPA) could be met by consuming a single ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.4786
更新日期:2020-05-30 00:00:00
abstract::Continuous subchronic exposure experiments were conducted to assess the effects of diazinon, an organophosphate pesticide, on the survival, growth and activity of larvae of the Asian common toad Bufo melanostictus. Two larval stages, the gill stage (Gosner stages 21 and 22) and gill-atrophy stage (Gosner stages 24 and...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/07-315.1
更新日期:2008-11-01 00:00:00
abstract::A model for risk assessment was built for simultaneous, congener-specific PCB bioaccumulation from sediment to fish to otters (Lutra lutra). Toxic equivalence factors (TEFs) were used to sum individual congeners in otters to a toxic equivalent concentration (TEQ) relative to 2,3,7,8-tetrachlorodibenzo-p-dioxin. Microb...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::It has been argued that current approaches for ecological risk assessment (ERA) do not provide value relevance for risk managers and that the uncertainty in the predictions is large. One important reason for this is that current approaches are based on individual-level endpoints, but the environmental protection goals...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.636
更新日期:2011-10-01 00:00:00