The role of toxic nectar secondary compounds in driving differential bumble bee preferences for milkweed flowers.

Abstract:

:While morphological differences such as tongue length are often featured as drivers of pollinator floral preferences, differences in chemical detection and tolerance to secondary compounds may also play a role. We sought to better understand the role of secondary compounds in floral preference by examining visitation of milkweed flowers, which can contain toxic cardenolides in their nectar, by bumble bees (Bombus spp.), some of their most abundant and important pollinators. We examine bumble bee species visitation of common milkweed (Asclepias syriaca) compared to other flowers in the field and test whether observed preferences may be influenced by avoidance and tolerance of cardenolides, as measured by the cardenolide ouabain, in the lab. We reveal that common milkweed is visited predominantly by one bumble bee species, Bombus griseocollis, in a ratio much higher than the abundance of this species in the community. We confirmed the presence and toxicity of cardenolides in A. syriaca nectar. Lab experiments revealed that B. griseocollis, compared to the common bumble bees B. impatiens and B. bimaculatus, exhibit greater avoidance of cardenolides, but only at levels that start to induce illness, whereas the other species exhibit either no or reduced avoidance of cardenolides, resulting in illness and mortality in these bees. Toxicity experiments reveal that B. griseocollis also has a substantially higher tolerance for cardenolides than B. impatiens. Together, these results support a potential evolutionary association between B. griseocollis and milkweed that may involve increased ability to both detect and tolerate milkweed cardenolides.

journal_name

Oecologia

journal_title

Oecologia

authors

Villalona E,Ezray BD,Laveaga E,Agrawal AA,Ali JG,Hines HM

doi

10.1007/s00442-020-04701-0

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

619-630

issue

3

eissn

0029-8549

issn

1432-1939

pii

10.1007/s00442-020-04701-0

journal_volume

193

pub_type

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