Abstract:
:Atherosclerotic lesional macrophages express molecules that promote plaque progression, but lack of mechanisms to therapeutically target these molecules represents a major gap in translational cardiovascular research. Here, we tested the efficacy of a small interfering RNA (siRNA) nanoparticle (NP) platform targeting a plaque-destabilizing macrophage molecule-Ca2+/calmodulin-dependent protein kinase γ (CaMKIIγ). CaMKIIγ becomes activated in advanced human and mouse plaque macrophages and drives plaque necrosis by suppressing the expression of the efferocytosis receptor MerTK. When macrophage-targeted siCamk2g NPs were administered to Western diet-fed Ldlr-/- mice, the atherosclerotic lesions showed decreased CaMKIIγ and increased MerTK expression in macrophages, improved phagocytosis of apoptotic cells (efferocytosis), decreased necrotic core area, and increased fibrous cap thickness-all signs of increased plaque stability-compared with mice treated with control siRNA NPs. These findings demonstrate that atherosclerosis-promoting genes in plaque macrophages can be targeted with siRNA NPs in a preclinical model of advanced atherosclerosis.
journal_name
Sci Transl Medjournal_title
Science translational medicineauthors
Tao W,Yurdagul A Jr,Kong N,Li W,Wang X,Doran AC,Feng C,Wang J,Islam MA,Farokhzad OC,Tabas I,Shi Jdoi
10.1126/scitranslmed.aay1063subject
Has Abstractpub_date
2020-07-22 00:00:00issue
553eissn
1946-6234issn
1946-6242pii
12/553/eaay1063journal_volume
12pub_type
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