Improvement of a Rapid and Highly Sensitive Method for the Diagnosis of the Mitochondrial m.1555A>G Mutation Based on a Single-Stranded Tag Hybridization Chromatographic Printed-Array Strip.


: Aims: Pathogenic variants in mitochondrial DNA are known to be associated with sensorineural hearing loss (SNHL) and aminoglycoside-induced HL. Among them, the m.1555A>G mutation is the most common. Thus, a rapid and easy companion diagnostic method for this mutation would be desirable to prevent HL caused by aminoglycoside therapy. In this study, we report an improved protocol for the single-stranded tag hybridization chromatographic printed-array strip (STH-PAS) method for identifying the m.1555A>G mutation. Methods: To evaluate the accuracy of a novel diagnostic for the m.1555A>G mutation we analyzed 378 DNA samples with or without the m.1555A>G mutation, as determined by Invader assay, and calculated the sensitivity, specificity, and false negative and false positive ratios of this new method. Results: The newly developed protocol was robust; we, obtained the same results using multiple DNA concentrations, differing annealing temperatures, and different polymerase chain reaction thermal cyclers. The diagnostic sensitivity based on the STH-PAS method was 0.99, and the specificity was 1.00. The false negative and false positive ratios were 0 and 0.01, respectively. Conclusion: We improved the genotyping method for m.1555A>G mutations. This assays will be useful as a rapid companion diagnostic before aminoglycoside use.


Isaka Y,Nishio SY,Hishinuma E,Hiratsuka M,Usami SI




Has Abstract


2021-01-01 00:00:00












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    abstract::Up to date, few published studies indicated the associations between genetic polymorphisms and epidural local anesthetics consumption. In this study, we investigated the associations between seven single-nucleotide polymorphisms (SNPs) and epidural ropivacaine consumption during breast cancer surgery in women from nor...

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    pub_type: 杂志文章


    authors: Dagmura H,Yiğit S,Nursal AF,Duman E,Gumusay O

    更新日期:2020-12-29 00:00:00

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    journal_title:Genetic testing and molecular biomarkers

    pub_type: 杂志文章


    authors: Lin CC,Wu CT,Wu LS

    更新日期:2011-04-01 00:00:00

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    journal_title:Genetic testing and molecular biomarkers

    pub_type: 杂志文章,meta分析


    authors: Zhao J,Chen ZY,Li LF

    更新日期:2019-05-01 00:00:00

  • Synergistic cytogenetic and antineoplastic effects by the combined action of esteric steroidal derivatives of nitrogen mustards.

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    journal_title:Genetic testing and molecular biomarkers

    pub_type: 杂志文章


    authors: Mourelatos C,Nikolaropoulos S,Fousteris M,Pairas G,Argyraki M,Kareli D,Dafa E,Mourelatos D,Lialiaris T

    更新日期:2012-06-01 00:00:00

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    journal_title:Genetic testing and molecular biomarkers

    pub_type: 临床试验,杂志文章


    authors: Wang X,Wang HQ,Jian T,Qin WW,Xu F,Xin ZL,Wang YH,Zhang M,Lu HJ

    更新日期:2015-12-01 00:00:00

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    journal_title:Genetic testing and molecular biomarkers

    pub_type: 临床试验,杂志文章


    authors: Papazoglou D,Restas E,Papanas N,Papatheodorou K,Babouris C,Glaros D,Antonoglou C,Maltezos E

    更新日期:2012-02-01 00:00:00

  • Next-Generation Testing for Cancer Risk: Perceptions, Experiences, and Needs Among Early Adopters in Community Healthcare Settings.

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    journal_title:Genetic testing and molecular biomarkers

    pub_type: 杂志文章


    authors: Blazer KR,Nehoray B,Solomon I,Niell-Swiller M,Culver JO,Uman GC,Weitzel JN

    更新日期:2015-12-01 00:00:00