Antioxidant activity of humic substances via bioluminescent monitoring in vitro.

Abstract:

UNLABELLED:This work considers antioxidant properties of natural detoxifying agents-humic substances (HS) in solutions of model inorganic and organic compounds of oxidative nature-complex salt K3[Fe(СN)6] and 1,4-benzoquinone. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H:FMN-oxidoreductase and bacterial luciferase was used as a bioassay in vitro to monitor toxicity of the oxidizer solutions. Toxicities of general and oxidative types were evaluated using bioluminescent kinetic parameters-bioluminescence intensity and induction period, respectively. Antioxidant activity of HS was attributed to their ability to decrease both general and oxidative toxicities; the HS antioxidant efficiency was characterized with detoxification coefficients D GT and D OxT, respectively. Dependencies of D GT and D OxT on HS concentration and time of preliminary incubation of the oxidizers with HS were demonstrated. The optimal conditions for detoxification of the oxidizers were >20-min incubation time and 0.5 × 10(-4) to 2 × 10(-4) M of HS concentration. The present study promotes application of the enzymatic luminescent bioassay to monitor toxicity of pollutants of oxidative nature in environmental and waste waters in remediation procedures.

journal_name

Environ Monit Assess

authors

Tarasova AS,Stom DI,Kudryasheva NS

doi

10.1007/s10661-015-4304-1

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

89

issue

3

eissn

0167-6369

issn

1573-2959

journal_volume

187

pub_type

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