Abstract:
INTRODUCTION:Histotripsy is a nonthermal, noninvasive, pulsed ultrasound technology that homogenizes tissue within the targeted volume. From previous experiments, it appeared that the resultant fibrotic response from histotripsy was limited compared with the typical tissue response seen after thermoablation. The objective of this study was to characterize the inflammatory response and quantify patterns of collagen deposition 6 weeks after in vivo canine prostate histotripsy. METHODS:Histotripsy was applied to the left half of eight canine prostates to produce an intraparenchymal zone of tissue homogenization. Six weeks after treatment, prostates were harvested, sectioned, and stained with hematoxylin and eosin for histologic evaluation, CD3, CD20, and Mac387 immunohistochemistry to characterize the inflammatory components, and picrosirius red staining to identify collagen. RESULTS:Seven of eight treated prostates exhibited only minimal residual inflammation. Visual microscopic analysis of picrosirius red slides revealed a band of dense collagen (0.5 mm wide) immediately adjacent to the cavity produced by histotripsy. This was surrounded by a second band (1 mm wide) of less dense collagen interspersed among glandular architecture. A lobar distribution of epithelial atrophy and basal cell hyperplasia reminiscent of periurethral glands and ducts was apparent surrounding the margin of the treatment cavities. Tissue loss (-31%) was apparent on the treated side of all prostates while four demonstrated a net decrease in collagen content. CONCLUSIONS:In vivo histotripsy of canine prostate produced a decrease in prostate volume coupled with a limited inflammatory and fibrotic response. A narrow (1.5 mm) band of fibrosis around the empty, reepithelialized treatment cavity was observed 6 weeks after treatment. In four cases, an overall reduction in collagen content was measured. Further studies are planned to correlate these histologic findings with alteration in mechanical tissue properties and to explore histotripsy strategies for treatment of benign prostatic hyperplasia that optimize tissue volume removal with minimization of fibrosis.
journal_name
J Endouroljournal_title
Journal of endourologyauthors
Darnell SE,Hall TL,Tomlins SA,Cheng X,Ives KA,Roberts WWdoi
10.1089/end.2014.0585subject
Has Abstractpub_date
2015-07-01 00:00:00pages
810-5issue
7eissn
0892-7790issn
1557-900Xjournal_volume
29pub_type
杂志文章abstract:BACKGROUND AND PURPOSE:Patients with spinal neuropathy are at an increased risk for urolithiasis. Data on percutaneous nephrolithotomy (PCNL) in this population are limited. Our objective is to review our experience in managing stones with PCNL in patients with spinal neuropathy. PATIENTS AND METHODS:Twenty-one patien...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2012.0344
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:The aim of our study was to evaluate if there is any advantage of three-dimensional helical computed tomography (3D-HCT) over intravenous urogram (IVU) in the morphometric and morphological analysis of lower pole spatial anatomy of the kidney. PATIENTS AND METHODS:We analyzed 52 renal collecting systems in 3...
journal_title:Journal of endourology
pub_type: 临床试验,杂志文章
doi:10.1089/end.2009.0262
更新日期:2009-12-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Radiation exposure during medical procedures continues to be an increasing concern for physicians and patients. We determined organ-specific dose rates and calculated effective dose rates during right and left percutaneous nephrolithotomy (PCNL) using a validated phantom model. MATERIALS AND MET...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2011.0178
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND AND PURPOSE:It is known that extracorporeal shock wave lithotripsy (SWL) may be improved by increasing cavitation using tandem shock waves. However, fluid is needed for the cavitation to work. The purpose of this research was to evaluate the importance of a fluid-filled expansion chamber in enhancing stone f...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2008.0334
更新日期:2009-04-01 00:00:00
abstract:OBJECTIVE:Preoperative percutaneous nephrostomy (PCN) has been commonly performed for patients with urinary stones complicated with pyelonephritis or obstructive uropathy; in fact, a considerable number of patients undergo ureteroscopic removal of stone (URS) in the presence of a PCN. We assessed the effectiveness of P...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2013.0347
更新日期:2013-10-01 00:00:00
abstract:PURPOSE:To assess the degree of postoperative storage symptoms after GreenLight™ laser photoselective vaporization of the prostate (PVP) and Holmium laser enucleation of the prostate (HoLEP) for management of benign prostatic hyperplasia (BPH) and its predictors. METHODS:A retrospective review was performed for patien...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2015.0202
更新日期:2015-10-01 00:00:00
abstract:PURPOSE:Burst wave lithotripsy (BWL) is a transcutaneous technique with potential to safely and effectively fragment renal stones. Preclinical investigations of BWL require the assessment of potential renal injury. This study evaluates the capabilities of real-time ultrasound and MRI to detect and evaluate BWL injury t...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2017.0202
更新日期:2017-08-01 00:00:00
abstract::To assess the durability of the results of transurethral microwave thermotherapy (TUMT) for symptomatic benign prostatic hyperplasia (BPH), we have reviewed publications describing trials with at least 3 years of follow-up. For men treated only by TUMT, improvement in symptoms and quality of life appears to be maintai...
journal_title:Journal of endourology
pub_type: 杂志文章,meta分析
doi:10.1089/end.2000.14.671
更新日期:2000-10-01 00:00:00
abstract:BACKGROUND AND PURPOSE:There is no uniform consensus regarding the anatomic factors that influence the clearance rate of caliceal stones after SWL, as different authors have studied various independent factors separately. We correlated both favorable and unfavorable factors into a formula to predict the clearance rate....
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2006.20.248
更新日期:2006-04-01 00:00:00
abstract:PURPOSE:The preliminary results of laparoscopic radical cystectomy in 85 patients are presented in this study. The functional and oncologic outcomes of this procedure in these patients are discussed. PATIENTS AND METHODS:Between December 2002 and May 2006, we performed 85 laparoscopic radical cystectomies with orthoto...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2007.0298
更新日期:2008-05-01 00:00:00
abstract::We describe our experience using an iodinated contrast solution to hydrodissect adjacent structures before percutaneous renal cryoablation. Hydrodissection was performed before cryoablation with placement of a 20-gauge, 15-cm introducer needle into the retroperitoneum under CT or ultrasonographic guidance followed by ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2011.0516
更新日期:2012-05-01 00:00:00
abstract::Transurethral resection of bladder tumor (TURBT) is the standard of care for initial bladder tumor management. In response to its shortcomings, we propose an alternative technique for tumor resection and retrieval: The endoscopic snare resection of bladder tumor (ESRBT). Eleven tumors managed by ESRBT were reviewed re...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2011.0587
更新日期:2012-06-01 00:00:00
abstract:PURPOSE:To evaluate prospectively the actual bladder perforation incidence during transurethral resection of bladder tumor (TURB) performed by residents and to identify possible predisposing factors to such condition. PATIENTS AND METHODS:Thirty-four patients with bladder tumor were submitted to TURB in our academic i...
journal_title:Journal of endourology
pub_type: 临床试验,杂志文章
doi:10.1089/end.2008.0406
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Ureteral stents are commonly used in urology today, but the biofilms that form on them within hours of placement may harbor bacteria that can result in infection or encrustation. Triclosan is an antimicrobial commonly used in consumer and medical products that inhibits bacterial fatty-acid synthe...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2006.20.949
更新日期:2006-11-01 00:00:00
abstract:PURPOSE:To define factors associated with the failure of shockwave lithotripsy (SWL) in the treatment of ureteral stones. PATIENTS AND METHODS:We retrospectively studied 405 men and 283 women (mean age 52.6 years) who underwent SWL with a second-generation lithotripter in the period 1994 to 2001. We evaluated availabl...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/089277903765444302
更新日期:2003-05-01 00:00:00
abstract:OBJECTIVE:To assess if the presence of a lower pole stone (LPS) decreases the stone-free (SF) rate following retrograde intrarenal surgery (RIRS). The second purpose was to assess the result of RIRS for LPS and to identify predictors of SF status. PATIENTS AND METHODS:We retrospectively analyzed 205 procedures in 162 ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2013.0515
更新日期:2014-02-01 00:00:00
abstract:PURPOSE:Rapid prototyping is a technique used for creating computer images in three dimensions more efficiently than classic techniques. Percutaneous nephrolithotomy (PCNL) is a popular method to remove kidney stones; however, broader use by the urologic community has been hampered by the morbidity associated with need...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2007.0025
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Benign ureteral strictures are a potentially difficult problem that typically has been solved by open surgery. However, minimally invasive methods would be preferable. PATIENTS AND METHODS:Three patients with benign ureteral strictures were treated with endoscopic placement of self-expanding permanent indwe...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/08927790050152186
更新日期:2000-09-01 00:00:00
abstract:PURPOSE:To determine whether the overall ureteral flow through an obstructed ureter using the 3F MicroStent™ that uses a novel film occlusion anchoring mechanism is comparable to the flow using a conventional 3F and 4.7F Double-J stent. MATERIALS AND METHODS:An in vitro silicone ureter model and an ex vivo porcine uri...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2010.0722
更新日期:2011-06-01 00:00:00
abstract:PURPOSE:To determine whether minimally invasive PCNL (MPCNL) is as safe and effective in the management of complex renal caliceal stones as it is for simple renal stones. PATIENTS AND METHODS:We retrospectively reviewed 5761(41.2%) simple caliceal stones (isolated renal pelvis including isolated calix) and 8223 (58.8%...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2013.0061
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND AND PURPOSE:The centrality-index (C-Index), preoperative aspects and dimensions used for anatomic (P.A.D.U.A.) classification, and radius.exophyic/endophytic.nearness.anterior/posterior.location (R.E.N.A.L.) nephrometry schemes were developed as standardized scoring systems (SS) to quantify anatomic characte...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2011.0301
更新日期:2011-12-01 00:00:00
abstract:PURPOSE:To evaluate the safety and oncologic efficacy of extravesical laparoscopic stapling of the distal ureter and bladder cuff during nephroureterectomy for pelvicaliceal transitional-cell carcinoma (TCC). PATIENTS AND METHODS:Patients with primary pelvicaliceal TCC and no history of TCC of the bladder or ureter wh...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2006.0306
更新日期:2007-09-01 00:00:00
abstract:INTRODUCTION:Renal papillary pits are commonly encountered during ureteroscopy. The mechanism by which such pits arise is unclear. One hypothesis is that pits represent sites where stones overgrowing Randall's plaque (RP) were dislodged. We sought to examine this theory by using digital ureteroscopy and stone μCT. MAT...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2018.0589
更新日期:2019-04-01 00:00:00
abstract:: Objective: To evaluate whether reducing the dose of fluoroscopy to ¼ of standard dose during unilateral ureteroscopy for ureteral stone treatment would impact in a reduction of total radiation emitted and whether this strategy would impact operation time, stone-free rate, ...
journal_title:Journal of endourology
pub_type: 杂志文章,随机对照试验
doi:10.1089/end.2018.0722
更新日期:2019-07-01 00:00:00
abstract::After an extensive favorable experience with the Lapides-Ball open retropubic bladder neck suspension, we chose to perform this operation laparoscopically. We describe video-assisted extraperitoneal laparoscopic bladder neck suspension (VELBNS), in which we employ a single laparoscopic cannula and a laparoscopic bladd...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.1995.9.259
更新日期:1995-06-01 00:00:00
abstract::A new technique of endosurgical pelvic lymph node dissection was performed for the staging of 10 prostate and 8 bladder cancers. The technique, involving an exclusive extraperitoneal space development with CO2 insufflation, is described in detail. Using the standard endosurgical (laparoscopic) equipment, we performed ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.1993.7.229
更新日期:1993-06-01 00:00:00
abstract:: Objective: Kidney stone formers are at risk for opioid dependence. The aim of this study is to describe opiate exposure and determine predictors of prolonged opiate use among kidney stone formers after surgery. Materials and Methods: ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2018.0796
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:To assess the effectiveness of periurethral microballoon implantation for management of female urinary incontinence. PATIENTS AND METHODS:A total of 45 women with urinary incontinence (age range 47-88 years) were treated between May 2000 and June 2001. Microballoons were placed in the proximal periurethral tis...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2004.18.901
更新日期:2004-11-01 00:00:00
abstract:OBJECTIVE:To assess the frequency of minimally invasive radical cystectomy (MIRC) conversion to open surgery, what factors influence conversion, whether or not the benefits of MIRC vs open radical cystectomy (ORC) persist after conversion, and compare ORC and MIRC outcomes. MATERIALS AND METHODS:We performed a retrosp...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2018.0079
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Laparoscopic radical nephrectomy (LRN) has benefits in terms of cosmesis, less postoperative pain, hospital stay, and early recovery when compared with open radical nephrectomy. One of the major points of concern of this surgery, especially in developing countries, is the additional cost because ...
journal_title:Journal of endourology
pub_type: 杂志文章
doi:10.1089/end.2008.0235
更新日期:2009-03-01 00:00:00