A two-step process for efficient enzymatic saccharification of rice straw.

Abstract:

:Response surface methodology was used to optimise a two-step process of cellulase mediated saccharification of rice straw by an isolated bacterium Lysinibacillus sphaericus. CMC concentration, yeast extract, pH and incubation temperature were optimised for cellulase production using a central composite design and their optimum values were determined to be 4.3% (w/v), 2.1% (w/v), 6.2 and 45.2 °C respectively. The CMCase activity at these values was 5.16±0.07 U/ml, which was 2.5 times that of the un-optimised system. Similarly, pretreated rice straw, enzyme load, incubation time and Tween-80 concentrations were optimised for enhanced saccharification of rice straw by optimised cellulase preparations, and their optimum values were calculated as 1.84% (w/v), 40 U, 57.4 h and 0.76 mM respectively. A percent saccharification of 69.5% was reported at optimal conditions. HPLC analysis revealed that hydrolysate produced at optimal conditions of saccharification constituted 70.8% of glucose.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Gupta P,Parkhey P

doi

10.1016/j.biortech.2014.09.101

subject

Has Abstract

pub_date

2014-12-01 00:00:00

pages

207-215

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(14)01360-1

journal_volume

173

pub_type

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