Abstract:
:Response surface methodology was used to optimise a two-step process of cellulase mediated saccharification of rice straw by an isolated bacterium Lysinibacillus sphaericus. CMC concentration, yeast extract, pH and incubation temperature were optimised for cellulase production using a central composite design and their optimum values were determined to be 4.3% (w/v), 2.1% (w/v), 6.2 and 45.2 °C respectively. The CMCase activity at these values was 5.16±0.07 U/ml, which was 2.5 times that of the un-optimised system. Similarly, pretreated rice straw, enzyme load, incubation time and Tween-80 concentrations were optimised for enhanced saccharification of rice straw by optimised cellulase preparations, and their optimum values were calculated as 1.84% (w/v), 40 U, 57.4 h and 0.76 mM respectively. A percent saccharification of 69.5% was reported at optimal conditions. HPLC analysis revealed that hydrolysate produced at optimal conditions of saccharification constituted 70.8% of glucose.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Gupta P,Parkhey Pdoi
10.1016/j.biortech.2014.09.101subject
Has Abstractpub_date
2014-12-01 00:00:00pages
207-215eissn
0960-8524issn
1873-2976pii
S0960-8524(14)01360-1journal_volume
173pub_type
杂志文章abstract::Manure from medicated livestock contains pharmaceutical antibiotics and antibiotic resistance genes (ARGs). Bioavailable antibiotics trigger further ARGs amplification during manure storage. It was tested whether biochar lowers the bioavailability of the antibiotics sulfamethazine (SMZ), ciprofloxacin (CIP), oxytetrac...
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.07.069
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