Semi-xenotransplantation: the regenerative medicine-based approach to immunosuppression-free transplantation and to meet the organ demand.

abstract：:Allogeneic hematopoietic stem cell transplantation (HSCT) and xenotransplantation are accompanied by viral reactivations and virus-associated complications resulting from immune deficiency. Here, in a Mauritian cynomolgus macaque model of fully MHC-matched allogeneic HSCT, we report reactivations of cynomolgus polyoma...

journal_title：Xenotransplantation

authors： Wu HL,Weber WC,Shriver-Munsch C,Swanson T,Northrup M,Price H,Armantrout K,Robertson-LeVay M,Reed JS,Bateman KB,Mahyari E,Thomas A,Junell SL,Hobbs TR,Martin LD,MacAllister R,Bimber BN,Slifka MK,Legasse AW,Moats C,A

更新日期：2020-07-01 00:00:00

abstract：:Cultures of hDAF transgenic porcine aortic endothelial cells (TPAEs) with levels of cell surface hDAF expression between 2000 and 300 000 molecules per cell have been used to determine the relationship between expression of hDAF and protection from human complement deposition in an in vitro model. At concentrations be...

journal_title：Xenotransplantation

authors： Carrington CA,dos Santos Cruz G

更新日期：2001-05-01 00:00:00

abstract：BACKGROUND:As a step towards clinical cardiac xenotransplantation, our experimental heterotopic intrathoracic xenotransplantation model offers a beating and ejecting donor heart while retaining the recipient's native organ as a backup in case of graft failure. Clinically applicable immunosuppressive regimens (IS) were ...

journal_title：Xenotransplantation

authors： Abicht JM,Mayr T,Reichart B,Buchholz S,Werner F,Lutzmann I,Schmoeckel M,Bauer A,Thormann M,Langenmayer M,Herbach N,Pohla H,Herzog R,McGregor CG,Ayares D,Wolf E,Klymiuk N,Baehr A,Kind A,Hagl C,Ganswindt U,Belka C

更新日期：2015-11-01 00:00:00

abstract：BACKGROUND:In vivo xenotransplantation modeling in large animal species is often performed in nonhuman primates, including baboons. For proper data interpretation, reference values for clinical chemistry and hematology are required. METHODS:These values are available from baseline levels in animals subjected to tolera...

journal_title：Xenotransplantation

authors： Schuurman HJ,Smith HT,Cozzi E

更新日期：2004-11-01 00:00:00

abstract：BACKGROUND:Islet transplantation is potentially a promising therapy for the restoration of carbohydrate control to diabetic patients. However, the global application of islet transplantation requires a ubiquitous source of beta cells. The xenotransplantation of porcine islets would provide such a source. Success in por...

journal_title：Xenotransplantation

authors： Pepper AR,Gall C,Mazzuca DM,Melling CW,White DJ

更新日期：2009-11-01 00:00:00

abstract：BACKGROUND:Manipulating the pig genome to increase compatibility with human biology may facilitate the clinical application of xenotransplantation. Genetic modifications to pig cells have been made by sequential recombination in fetal fibroblasts and liver-derived cells followed by cross-breeding or somatic cell nuclea...

journal_title：Xenotransplantation

authors： Li P,Estrada JL,Burlak C,Montgomery J,Butler JR,Santos RM,Wang ZY,Paris LL,Blankenship RL,Downey SM,Tector M,Tector AJ

更新日期：2015-01-01 00:00:00

abstract：:Type 1 diabetes can be cured by transplantation of isolated pancreatic islets. Because of the shortage of human donor tissue, adult porcine islets (APIs) constitute a possible alternative tissue source. Upon intraportal injection, islets are subjected to an instant blood-mediated inflammatory reaction (IBMIR) leading ...

journal_title：Xenotransplantation

authors： Ehrnfelt C,Kumagai-Braesch M,Uzunel M,Holgersson J

更新日期：2004-03-01 00:00:00

abstract：:The first blood transfusions in humans were xenotransfusions, carried out by Jean-Baptiste Denis beginning in 1667. Richard Lower, Matthäus Purmann and Georges Mercklin also experimented with the use of animal blood for transfusion until this practice was forbidden in 1670, after the death of one of Denis's patients. ...

journal_title：Xenotransplantation

authors： Roux FA,Saï P,Deschamps JY

更新日期：2007-05-01 00:00:00

abstract：:Highly sensitized patients awaiting kidney transplantation may be potential candidates for future clinical trials using pig organ donors. Because of crossreactivity between human leucocyte antigens (HLA) and swine leucocyte antigens (SLA), such patients might have heightened T-cell responses to porcine xenoantigens. W...

journal_title：Xenotransplantation

authors： Oostingh GJ,Davies HF,Bradley JA,Taylor CJ

更新日期：2003-11-01 00:00:00

abstract：BACKGROUND:Human cytomegalovirus (HCMV) infection or reactivation has been linked to allograft rejection resulting from endothelial injury and immune activation. In pig-to-human xenotransplantation, currently investigated to circumvent the shortage of human organs in transplantation medicine, the porcine endothelium wi...

journal_title：Xenotransplantation

authors： Millard AL,Häberli L,Sinzger C,Ghielmetti M,Schneider MK,Bossart W,Seebach JD,Mueller NJ

更新日期：2010-07-01 00:00:00

abstract：AIM:to study whether sensitization to pig antigens results in humoral and/or cellular sensitization to alloantigens in baboons, and thus increases the risks of organ allotransplantation after xenotransplantation. Serum from baboons that were naive (n = 4), sensitized to Gal alpha 1,3Gal (Gal) antigens (n = 2), or sensi...

journal_title：Xenotransplantation

authors： Baertschiger RM,Dor FJ,Prabharasuth D,Kuwaki K,Cooper DK

更新日期：2004-01-01 00:00:00

abstract：:Fetal pig islets, xenografted after organ culture into non-immunosuppressed prediabetic NOD mice, are rejected within 10 days. Immunosuppression with anti-T cell (anti-CD4 and anti-CD3) monoclonal antibodies alone is highly effective in delaying graft rejection in this discordant model, but rejection eventually occurs...

journal_title：Xenotransplantation

authors： Koulmanda M,Kovarik J,Mandel TE

更新日期：1998-08-01 00:00:00

abstract：BACKGROUND:Xenotransplantation of porcine islets can reverse diabetes in non-human primates. The remaining hurdles for clinical application include safe and effective T-cell-directed immunosuppression, but protection against the innate immune system and coagulation dysfunction may be more difficult to achieve. Islet-ta...

journal_title：Xenotransplantation

authors： Wijkstrom M,Bottino R,Iwase H,Hara H,Ekser B,van der Windt D,Long C,Toledo FG,Phelps CJ,Trucco M,Cooper DK,Ayares D

更新日期：2015-01-01 00:00:00

abstract：:The aim of the present study was to evaluate the immunosuppressive effect of tacrolimus (TAC) in discordant islet xenotransplantation. Fetal porcine islet-like cell clusters (ICCs) were transplanted under the kidney capsule in normoglycemic rats treated with TAC monotherapy, TAC plus other immunosuppressive drugs or c...

journal_title：Xenotransplantation

authors： Song Z,Zhang J,Bennet W,Wennberg L

更新日期：2003-11-01 00:00:00

abstract：:Neonatal porcine islets are characterized by reproducible isolation success and high yields, sizable advantages over adult islets. In this work we have analyzed selected phenotypic and functional characteristics of porcine neonatal islets relevant to their possible use for transplant in humans. We show that porcine is...

journal_title：Xenotransplantation

authors： Vizzardelli C,Molano RD,Pileggi A,Berney T,Cattan P,Fenjves ES,Peel A,Fraker C,Ricordi C,Inverardi L

更新日期：2002-01-01 00:00:00

abstract：UNLABELLED:Xenotransplantation using porcine organs may resolve a chronic shortage of donor organs for clinical transplantation if significant immunological barriers can be overcome. To determine the potential role of T lymphocytes in Xenograft (Xg) rejection, we transplanted transgenic hCD46 porcine hearts heterotopic...

journal_title：Xenotransplantation

authors： Davila E,Byrne GW,LaBreche PT,McGregor HC,Schwab AK,Davies WR,Rao VP,Oi K,Tazelaar HD,Logan JS,McGregor CG

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Swine infectious pathogens, especially viruses, represent a potential public health risk associated with the use of pig tissues for xenotransplantation in humans. We hypothesized that porcine circovirus type I (PCV-1) may infect human mononuclear cells, resulting in ultrastructural alterations of the target ...

journal_title：Xenotransplantation

authors： Arteaga-Troncoso G,Guerra-Infante F,Rosales-Montaño LM,Díaz-García FJ,Flores-Medina S

更新日期：2005-11-01 00:00:00

abstract：:The effects of pre-transplant blood transfusion vary from induction of antibodies and accelerated graft rejection, to prolonged survival and even tolerance. The beneficial 'transfusion effect' in allotransplantation is believed to be merely T-cell mediated. In xenotransplantation, T-cell independent mechanisms form a ...

journal_title：Xenotransplantation

authors： Vriens PW,Stoot JH,Hoyt G,Scheringa M,Bouwman E,Robbins RC

更新日期：2001-11-01 00:00:00

abstract：:Previous data suggest that natural anti-Galalpha1,3Gal (Gal) antibody (Ab) is produced by mature plasma cells. As thalidomide is effectively used in the treatment of patients with multiple myeloma, its effect on natural anti-Gal Ab production has been assessed in two baboons. During a 10-week course of thalidomide adm...

journal_title：Xenotransplantation

authors： Yamamoto S,Cooper DK

更新日期：2003-09-01 00:00:00

abstract：:Development of coagulation disorders remains a major challenge in pig-to-primate organ xenotransplantation. Our previous studies demonstrated that porcine aortic endothelial cells (pAEC) activate human platelets to express tissue factor (TF). In this study, we investigated the molecular interaction between human plate...

journal_title：Xenotransplantation

authors： Ezzelarab MB,Liu YW,Lin CC,Long C,Ayares D,Dorling A,Cooper DK

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:Xenotransplantation using pig cells, tissues, or organs may be associated with the transmission of porcine microorganisms and the development of zoonoses. Among all porcine microorganisms porcine endogenous retroviruses (PERVs) represent a special risk because they are integrated in the genome of all pigs an...

journal_title：Xenotransplantation

authors： Wynyard S,Nathu D,Garkavenko O,Denner J,Elliott R

更新日期：2014-07-01 00:00:00

abstract：BACKGROUND:Microvascular thrombosis is a prominent characteristic of delayed xenograft rejection, therefore the effects of antiplatelet therapy with aspirin and clopidogrel on long-term cardiac xenograft function was investigated in a heterotopic pig-to-baboon cardiac transplant model. METHODS:Donor hearts from human ...

journal_title：Xenotransplantation

authors： Schirmer JM,Fass DN,Byrne GW,Tazelaar HD,Logan JS,McGregor CG

更新日期：2004-09-01 00:00:00

abstract：BACKGROUND:Pig islets represent an alternative to the current modes of treatment for patients with diabetes. However, the concerns over pathogen transmission including that of PERV limit their immediate, widespread usage in humans. It has been previously demonstrated that PERV copy number and particularly expression le...

journal_title：Xenotransplantation

authors： Mourad NI,Crossan C,Cruikshank V,Scobie L,Gianello P

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:Safe and reliable diabetes models are a key prerequisite for advanced preclinical studies on diabetes. Chemical induction is the standard model of diabetes in rodents and also widely used in large animal models of non-human primates and minipigs. However, uncertain efficacy, the potential of beta-cell regene...

journal_title：Xenotransplantation

authors： Heinke S,Ludwig B,Schubert U,Schmid J,Kiss T,Steffen A,Bornstein S,Ludwig S

更新日期：2016-09-01 00:00:00

abstract：:The last 15 years or so have seen exciting progress in xenotransplantation, with porcine organ grafts surviving months or even years in non-human primates. These advances reflect the application of new scientific knowledge, improved immunosuppressive agents, and genetic engineering. The field has recently enjoyed a re...

journal_title：Xenotransplantation

authors： Sykes M

更新日期：2018-05-01 00:00:00

abstract：:The use of xenogeneic porcine pancreatic islets has been shown to be a potentially promising alternative to using human allogeneic islets to treat insulin-dependent type 1 diabetes (T1D). This article provides an overview of the existing FDA regulatory framework that would be applied to the regulation of clinical tria...

journal_title：Xenotransplantation

authors： Arcidiacono JA,Evdokimov E,Lee MH,Jones J,Rudenko L,Schneider B,Snoy PJ,Wei CH,Wensky AK,Wonnacott K

更新日期：2010-09-01 00:00:00

abstract：BACKGROUND:We have successfully performed heart transplantation despite the most unfavourable risk factors for graft and patient survival: the presence of a high level of antibodies (Abs) against the donor's human leukocyte antigens (HLA) class I/II and blood group A1 antigens. The present study concerns post-transplan...

journal_title：Xenotransplantation

authors： Bućin D,Johansson S,Lindberg LO

更新日期：2006-03-01 00:00:00

abstract：:When clinical xenotransplantation is introduced, the costs associated with acquisition of a genetically engineered pig organ are as yet unknown. How will these costs compare with those currently associated with the acquisition of deceased human organs? An understanding of the financial aspects of deceased organ and ti...

journal_title：Xenotransplantation

authors： Saari RJ,Cooper DKC

更新日期：2017-07-01 00:00:00

abstract：:Xenotransplantion remains the most viable option for significant expansion of the donor organ pool in clinical transplantation. With the advent of nuclear transfer technologies, the production of transgenic swine has become a possibility. These animals have allowed transplant investigators to overcome humoral mechanis...

journal_title：Xenotransplantation

authors： Scalea J,Hanecamp I,Robson SC,Yamada K

更新日期：2012-01-01 00:00:00

abstract：BACKGROUND:Over time, the notion of public has evolved. While the concept of public was initially conceived of as a single undifferentiated entity, the common understanding is now that a variety of differentiated, multifaceted and multiple public(s) can be constructed for different purposes. This is equally true in xen...

journal_title：Xenotransplantation

authors： Sobbrio P,Jorqui M

更新日期：2014-11-01 00:00:00