T-cell-mediated immunological barriers to xenotransplantation.

abstract：:Islets isolated from multiple pancreas donors are often necessary to achieve euglycemia in type 1 diabetic patients treated by islet allotransplantation. This increases the burden on the limited pool of donor organs. After infusion into the portal vein, a substantial percentage of islets are lost in the immediate post...

journal_title：Xenotransplantation

authors： van der Windt DJ,Bottino R,Casu A,Campanile N,Cooper DK

更新日期：2007-07-01 00:00:00

abstract：BACKGROUND:Innovations in transgenic technology have facilitated improved xenograft survival. Additional gene expression appears to be necessary to overcome the remaining immune and biologic incompatibilities. We report for the first time the novel use of six-gene modifications within a pig-to-baboon cardiac xenotransp...

journal_title：Xenotransplantation

authors： Chan JL,Singh AK,Corcoran PC,Thomas ML,Lewis BG,Ayares DL,Vaught T,Horvath KA,Mohiuddin MM

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:Despite overcoming xenograft hyperacute rejection (HAR), Gal (galactose-alpha1,3-galactose) expression may not be completely eliminated from the alpha1,3-galactosyltransferase gene knockout (Gal KO) pig because of alternative galactosyltransferases. Whether low levels of "residual" Gal are still susceptible ...

journal_title：Xenotransplantation

authors： Murray-Segal L,Gock H,Cowan PJ,d'Apice AJ

更新日期：2008-02-01 00:00:00

abstract：:The present historical review reports the clinical experiences of transplantations from animal to human. The first transplantation attempts were made without any knowledge of the species barrier. The pioneers of xenotransplantation realized xenotransfusions as early as the 16th century, then cell and tissue xenotransp...

journal_title：Xenotransplantation

authors： Deschamps JY,Roux FA,Saï P,Gouin E

更新日期：2005-03-01 00:00:00

abstract：BACKGROUND:Whilst macrohemodynamic function of porcine xenografts transplanted into baboons has been assessed perioperatively, the ability of the xenograft to maintain systemic microcirculatory perfusion has not been investigated after pig-to-baboon xenotransplantation so far. METHODS:We investigated the sublingual mi...

journal_title：Xenotransplantation

authors： Bauer A,Renz V,Baschnegger H,Abicht JM,Beiras-Fernandez A,Brenner P,Thein E,Schmoeckel M,Reichart B,Christ F

更新日期：2011-07-01 00:00:00

abstract：BACKGROUND:Pig islets represent an alternative to the current modes of treatment for patients with diabetes. However, the concerns over pathogen transmission including that of PERV limit their immediate, widespread usage in humans. It has been previously demonstrated that PERV copy number and particularly expression le...

journal_title：Xenotransplantation

authors： Mourad NI,Crossan C,Cruikshank V,Scobie L,Gianello P

更新日期：2017-07-01 00:00:00

abstract：:Exposure to human serum induces the lysis of xenogeneic cells through natural antibodies and complement activation. The carbohydrate Galactose-alpha1,3-Galactose (Gal-alpha1,3-Gal) epitope, has been shown to be the principal antigenic determinant on target cells. This reaction is, therefore, particularly important for...

journal_title：Xenotransplantation

authors： Déglon N,Aubert V,Spertini F,Winkel L,Aebischer P

更新日期：2003-05-01 00:00:00

abstract：BACKGROUND:  Various groups have reported prolonged diabetes reversal and graft function after porcine islet transplantation into diabetic macaques using different experimental designs (macaque source, islet source, type of immunosuppression): subsequently, the International Xenotransplantation Association has publishe...

journal_title：Xenotransplantation

authors： Graham ML,Schuurman HJ

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:Intravascular thrombosis and systemic coagulation abnormalities are major hurdles to successful xenotransplantation and are signs of acute humoral rejection. Increased expression of tissue factor (TF) is associated with the development of microvascular thrombosis in xenografts. To develop an effective strate...

journal_title：Xenotransplantation

authors： Lee KG,Lee H,Ha JM,Lee YK,Kang HJ,Park CG,Kim SJ

更新日期：2012-05-01 00:00:00

abstract：BACKGROUND:The continued presence of a primate antibody-mediated response to cells and organs from alpha1,3-galactosyltransferase gene-knockout (GTKO) pigs indicates that there may be antigens other than Gal alpha 1,3Gal (alpha Gal) against which primates have xenoreactive antibodies. Human and baboon sera were tested ...

journal_title：Xenotransplantation

authors： Yeh P,Ezzelarab M,Bovin N,Hara H,Long C,Tomiyama K,Sun F,Ayares D,Awwad M,Cooper DK

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND:As a step towards clinical cardiac xenotransplantation, our experimental heterotopic intrathoracic xenotransplantation model offers a beating and ejecting donor heart while retaining the recipient's native organ as a backup in case of graft failure. Clinically applicable immunosuppressive regimens (IS) were ...

journal_title：Xenotransplantation

authors： Abicht JM,Mayr T,Reichart B,Buchholz S,Werner F,Lutzmann I,Schmoeckel M,Bauer A,Thormann M,Langenmayer M,Herbach N,Pohla H,Herzog R,McGregor CG,Ayares D,Wolf E,Klymiuk N,Baehr A,Kind A,Hagl C,Ganswindt U,Belka C

更新日期：2015-11-01 00:00:00

abstract：:Complement plays a major role in hyperacute rejection of xenografts. In order to overcome this, we are developing, by minimal mutagenesis, a modified C3 molecule that, like cobra venom factor (CVF), escapes normal complement regulatory processes and inhibits complement-mediated responses by systemic depletion of C3. U...

journal_title：Xenotransplantation

authors： Fecke W,Farries TC,D'Cruz LG,Napper CM,Harrison RA

更新日期：1998-02-01 00:00:00

abstract：BACKGROUND:In pig-to-human xenotransplantation, interactions between human natural killer (NK) cells and porcine endothelial cells (pEC) are characterized by recruitment and cytotoxicity. Protection from xenogeneic NK cytotoxicity can be achieved in vitro by the expression of the non-classical human leukocyte antigen-E...

journal_title：Xenotransplantation

authors： Puga Yung G,Bongoni AK,Pradier A,Madelon N,Papaserafeim M,Sfriso R,Ayares DL,Wolf E,Klymiuk N,Bähr A,Constantinescu MA,Voegelin E,Kiermeir D,Jenni H,Rieben R,Seebach JD

更新日期：2018-01-01 00:00:00

abstract：:The effects of pre-transplant blood transfusion vary from induction of antibodies and accelerated graft rejection, to prolonged survival and even tolerance. The beneficial 'transfusion effect' in allotransplantation is believed to be merely T-cell mediated. In xenotransplantation, T-cell independent mechanisms form a ...

journal_title：Xenotransplantation

authors： Vriens PW,Stoot JH,Hoyt G,Scheringa M,Bouwman E,Robbins RC

更新日期：2001-11-01 00:00:00

abstract：:Acute humoral rejection remains the major barrier to long-term pig-to-primate xenograft survival, and microvascular thrombosis is a critical element of the rejection process. It appears that persistent endothelial cell activation and injury, by even low levels of anti-graft antibodies, eventually overwhelm the cellula...

journal_title：Xenotransplantation

authors： Cowan PJ

更新日期：2007-01-01 00:00:00

abstract：:Type I diabetes remains a significant clinical problem in need of a reliable, generally applicable solution. Both whole organ pancreas and islet allotransplantation have been shown to grant patients insulin independence, but organ availability has restricted these procedures to an exceptionally small subset of the dia...

journal_title：Xenotransplantation

authors： Samy KP,Martin BM,Turgeon NA,Kirk AD

更新日期：2014-05-01 00:00:00

abstract：BACKGROUND:Porcine islet transplantation into diabetic non-human primates is considered most relevant in translational research supporting a clinical application. Most studies have focused on immunosuppressive protocols, while metabolic aspects have mainly been utilized in graft monitoring. We evaluated data from our g...

journal_title：Xenotransplantation

authors： Graham ML,Bellin MD,Papas KK,Hering BJ,Schuurman HJ

更新日期：2011-11-01 00:00:00

abstract：:The newborn infant with severe cardiac failure owed to congenital structural heart disease or cardiomyopathy poses a daunting therapeutic challenge. The ideal solution for both might be cardiac transplantation if availability of hearts was not limiting and if tolerance could be induced, obviating toxicity of immunosup...

journal_title：Xenotransplantation

authors： Platt JL,West LJ,Chinnock RE,Cascalho M

更新日期：2018-11-01 00:00:00

abstract：AIM:To provide information on the specificity of induced anti-pig antibodies (Abs) in baboons after exposure and sensitization to pig antigens. MATERIALS AND METHODS:Baboons (n=7) received either porcine mobilized peripheral blood progenitor cells (n=3), kidney (n=3) or heart (n=1) transplants. After rejection of thes...

journal_title：Xenotransplantation

authors： Buhler L,Xu Y,Li W,Zhu A,Cooper DK

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:In the α1,3-galactosyltransferase knockout (α-GalT KO) pig era, identification of the non-Gal epitopes is necessary for successful pig-to-human xenotransplantation. Recently, we successfully detected α-Gal epitopes as well as Hanganutziu-Deicher (H-D) antigens from the N-glycans in the pig heart tissues, whi...

journal_title：Xenotransplantation

authors： Jeong HJ,Adhya M,Park HM,Kim YG,Kim BG

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND:Little information is available regarding the precise swine leukocyte antigen (SLA)-derived immunogenic peptides that are presented in the context of human HLA molecules. Here, we identified SLA-derived immunogenic peptides that are presented in association with human HLA-A2 molecule. METHODS:The SLA-derive...

journal_title：Xenotransplantation

authors： Park CS,Im SA,Song S,Kim K,Lee CK

更新日期：2014-09-01 00:00:00

abstract：BACKGROUND:Recently, we have shown that an immunosuppression regimen including costimulation blockade via anti-CD154 antibody significantly prolongs the cardiac xenograft survival in a GTKO.hCD46Tg pig-to-baboon heterotopic xenotransplantation model. Unfortunately, many coagulation disorders were observed with the use ...

journal_title：Xenotransplantation

authors： Mohiuddin MM,Singh AK,Corcoran PC,Hoyt RF,Thomas ML 3rd,Lewis BG,Eckhaus M,Dabkowski NL,Belli AJ,Reimann KA,Ayares D,Horvath KA

更新日期：2014-01-01 00:00:00

abstract：:Orthotopic liver transplantation (OLT) is the only definitive treatment option for many patients with end-stage liver disease. Current supply of donor livers for OLT is not keeping up with the growing demand. To overcome this problem, a number of experimental strategies have been developed either to provide a bridge t...

journal_title：Xenotransplantation

authors： Aravalli RN

更新日期：2020-12-08 00:00:00

abstract：:The first blood transfusions in humans were xenotransfusions, carried out by Jean-Baptiste Denis beginning in 1667. Richard Lower, Matthäus Purmann and Georges Mercklin also experimented with the use of animal blood for transfusion until this practice was forbidden in 1670, after the death of one of Denis's patients. ...

journal_title：Xenotransplantation

authors： Roux FA,Saï P,Deschamps JY

更新日期：2007-05-01 00:00:00

abstract：BACKGROUND:Safe and reliable diabetes models are a key prerequisite for advanced preclinical studies on diabetes. Chemical induction is the standard model of diabetes in rodents and also widely used in large animal models of non-human primates and minipigs. However, uncertain efficacy, the potential of beta-cell regene...

journal_title：Xenotransplantation

authors： Heinke S,Ludwig B,Schubert U,Schmid J,Kiss T,Steffen A,Bornstein S,Ludwig S

更新日期：2016-09-01 00:00:00

abstract：:We designed this study to define reference values of the cynomolgus monkey coagulation system, as the normal range of values has not been established. Measurement of coagulation function was determined by testing plasma samples from 30 healthy male cynomolgus monkeys. Prothrombin time (PT), PT activity, PT internation...

journal_title：Xenotransplantation

authors： Rhu J,Lee KW,Kim KS,Lee JS,Kim SJ,Park JB

更新日期：2019-01-01 00:00:00

abstract：BACKGROUND:Young people, and students in particular, generally have a positive attitude to xenotransplantation. We reported previously that university students attending their first year approved of the idea of transplanting animal organs into humans. The aim of this study was to evaluate the impact of a 3-yr course at...

journal_title：Xenotransplantation

authors： Canova D,De Bona M,Rumiati R,Masier A,Ermani M,Naccarato R,Cozzi E,Ancona E,Burra P

更新日期：2006-05-01 00:00:00

abstract：:The infusion of large numbers of porcine cells into primates in order to induce specific immunologic tolerance by mixed hematopoietic chimerism, results in thrombotic microangiopathy that can be fatal. For this reason, it is important to study in vitro the interaction of primate endothelial cells with pig cells. We sh...

journal_title：Xenotransplantation

authors： Benatuil L,Fernandez AZ,Apitz-Castro R,Romano E

更新日期：2002-05-01 00:00:00

abstract：UNLABELLED:Xenotransplantation using porcine organs may resolve a chronic shortage of donor organs for clinical transplantation if significant immunological barriers can be overcome. To determine the potential role of T lymphocytes in Xenograft (Xg) rejection, we transplanted transgenic hCD46 porcine hearts heterotopic...

journal_title：Xenotransplantation

authors： Davila E,Byrne GW,LaBreche PT,McGregor HC,Schwab AK,Davies WR,Rao VP,Oi K,Tazelaar HD,Logan JS,McGregor CG

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Dysregulation of coagulation is considered a major barrier against successful pig organ xenotransplantation in non-human primates. Inflammation is known to promote activation of coagulation. The role of pro-inflammatory factors as well as the relationship between inflammation and activation of coagulation in...

journal_title：Xenotransplantation

authors： Ezzelarab MB,Ekser B,Azimzadeh A,Lin CC,Zhao Y,Rodriguez R,Echeverri GJ,Iwase H,Long C,Hara H,Ayares D,Pierson RN,Thomson AW,Cooper DK

更新日期：2015-01-01 00:00:00