The Dutch PCB/Dioxin Study. Development of a method for the determination of dioxins, planar and other PCBs in human milk.

Abstract:

:A method for the determination of dioxins, planar PCBs and other PCBs in human milk is described. The work is part of a big Dutch project, (seven institutes participates) in which 400 human milk samples from two areas (Rotterdam and Groningen) taken at two different times (10 and 42 days) after delivery had to be analysed for planar PCBs, dioxins and other PCBs (1st sampling) and only other PCBs (2nd sampling). Planar compounds are determined with GC/HRMS, other PCBs with GC/ECD. As type of analytes for 1st and 2nd sampling are different the method was focussed on these analytes. After fat extraction, clean up was carried out on GPC and alumina columns. Only in case of analysis of planar compounds and other PCBs, chromatography on porous graphitised carbon was carried out after which GC-HRMS (planar compounds) and GC/ECD (other PCBs) was performed; otherwise GC/ECD after alumina clean up was performed. The method is validated through recovery experiments with standards (other PCBs, labelled planar compounds), comparison of results for other PCBs obtained with the two different clean-up procedures, reproducibility of "quality control samples" for planar compounds blanc chemicals.

journal_name

Chemosphere

journal_title

Chemosphere

authors

Tuinstra LG,Traag WA,van Rhijn JA,vd Spreng PF

doi

10.1016/0045-6535(94)90352-2

subject

Has Abstract

pub_date

1994-11-01 00:00:00

pages

1859-75

issue

9-11

eissn

0045-6535

issn

1879-1298

pii

0045-6535(94)90352-2

journal_volume

29

pub_type

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