Effect of glutathione administration on hepatic biliary and plasmatic glutathione levels in the rat.

Abstract:

BACKGROUND:Since the effect of exogenous glutathione (GSH) on overall hepatic GSH homeostasis is not known, the present study investigated the changes in the hepatic, biliary, and plasmatic GSH levels during GSH administration in intact rats. METHODS:An exteriorized biliary-duodenal fistula was established, and GSH (1 mmol/kg over 2 h) or saline was administered intraperitoneally to rats with or without pretreatment with 5 mmol/kg L-serine borate, an inhibitor of gamma-glutamyltransferase (GGT). RESULTS:Three hours after GSH administration, biliary GSH efflux and bile flow rose from 104.7 +/- 5.6 to 290.6 +/- 8.6 micrograms/ml bile and from 20.2 +/- 1.3 to 30.2 +/- 2.1 microliters/min, respectively; GSH-treated rats also showed increased liver (35%) and posthepatic vein plasma (68%) GSH concentrations compared with controls. By contrast, in rats pretreated with the GGT inhibitor GSH administration appeared to be devoid of any effect, except for a modest biliary GSH increase. CONCLUSIONS:This study indicates that significant changes occur in the hepatic GSH homeostasis after intraperitoneal GSH administration. The activity of hepatic GGT, most likely through degradation of circulating GSH, followed by an increase in cysteine availability, seems to account, at least partially, for the reported effects.

journal_name

Scand J Gastroenterol

authors

Vendemiale G,Palmieri V,Palasciano G,Altomare E

doi

10.3109/00365529409094882

subject

Has Abstract

pub_date

1994-11-01 00:00:00

pages

1034-8

issue

11

eissn

0036-5521

issn

1502-7708

journal_volume

29

pub_type

杂志文章
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