Abstract:
:A pair of synthetic oligonucleotide primers, designed from the gene encoding a 32-kDa intraerythrocytic piroplasm surface protein of Theileria sergenti, were used to amplify parasite DNA from the blood of T. sergenti-infected cattle by means of the polymerase chain reaction (PCR). PCR-amplified DNA was examined by electrophoresis and by dot blot or microplate hybridization using a parasite-specific cDNA probe. PCR was specific for T. sergenti, since no amplification was detected with DNA from Anaplasma centrale, Babesia ovata, uninfected erythrocytes, and leukocytes. This method was sensitive enough to detect about 4.5 parasites per microliters of blood with a 10-microliters sample volume. Moreover, of 66 specimens from grazing cattle, 40 were microscopically positive, whereas PCR revealed that 54 samples were positive. Therefore, PCR provides a useful diagnostic tool for detecting T. sergenti-infected cattle, and it is significantly more sensitive than the current methods.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Tanaka M,Onoe S,Matsuba T,Katayama S,Yamanaka M,Yonemichi H,Hiramatsu K,Baek BK,Sugimoto C,Onuma Mdoi
10.1128/JCM.31.10.2565-2569.1993subject
Has Abstractpub_date
1993-10-01 00:00:00pages
2565-9issue
10eissn
0095-1137issn
1098-660Xjournal_volume
31pub_type
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doi:10.1128/JCM.27.4.622-627.1989
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
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