Abstract：
PURPOSE:HPV-related oropharyngeal squamous cell carcinomas clearly represent a growing entity in the head and neck with distinct carcinogenesis, clinico-pathological presentation and survival profile. We aimed to compare the HPV prevalence rates and clinico-pathological correlations obtained with three distinct commonly used HPV detection methods. MATERIALS AND METHODS:p16-immunohistochemistry (IHC), HPV DNA viral load by real-time PCR (qPCR), and HPV genotyping by a reverse hybridization-based line probe assay (INNO-LiPA) were performed on pretreatment formalin-fixed paraffin-embedded tumor samples from 46 patients treated for single primary oropharyngeal carcinomas. RESULTS:Twenty-eight patients (61%) had a p16 overexpression in IHC. Twenty-nine patients (63%) harbored HPV DNA on qPCR. Thirty-four patients (74%) harbored HPV DNA on INNO-LiPA. The concordance analysis revealed a good agreement between both HPV DNA detection methods (κ=0.65); when both tests were positive, the depicted HPV subtypes were always concordant (HPV16 in 27 cases, HPV18 in 1 case). Agreement was moderate between IHC and qPCR (κ=0.59) and fair between IHC and INNO-LiPA (κ=0.22). CONCLUSIONS:Certain highly sensitive methods are able to detect the mere presence of HPV without any carcinogenetic involvement while other more specific tests provide proof of viral transcriptional activity and thus evidence of clinically relevant infections. The use of a stepwise approach allows reducing false positives; p16-immunostaining seems to be an excellent screening test and in situ hybridization may overcome some of the PCR limitations.
journal_name
Am J Otolaryngoljournal_title
American journal of otolaryngologyauthors
Melkane AE,Mirghani H,Aupérin A,Saulnier P,Lacroix L,Vielh P,Casiraghi O,Griscelli F,Temam Sdoi
10.1016/j.amjoto.2013.08.007subject
Has Abstractpub_date
2014-01-01 00:00:00pages
25-32issue
1eissn
0196-0709issn
1532-818Xpii
S0196-0709(13)00198-1journal_volume
35pub_type
杂志文章abstract：PURPOSE:The aim of this study was to evaluate the vestibular functions and dual-task performances of individuals with noise induced hearing loss. MATERIALS AND METHODS:Fifty individuals with noise induced hearing loss and 25 healthy individuals were included in the study. Individuals with noise induced hearing loss we...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102665
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVE:In pituitary macroadenomas with extensive suprasellar extension (SSE), suprasellar region can be approached by extending exposure either anteriorly by adding bone removal of the tuberculum sellae and planum sphenoidal or posteriorly from inside the sellar cavity through diaphragma sella. The later approach ha...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102808
更新日期：2021-01-01 00:00:00
abstract：OBJECTIVES:In the literature, diabetes mellitus was mentioned as one of the etiologic factors of olfactory disorder. However, association between olfactory dysfunction and complications of type 2 diabetes mellitus is unclear. The aim of this study was to determine if there is any correlation between olfactory dysfuncti...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2019.102365
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:Pneumocephalus is a rare complication of endoscopic sinus surgery (ESS) and microscopic skull base surgery (MSBS). Postoperatively, patients often present with headache and altered mental status. Unrepaired leaks are associated with an increased risk of ascending meningitis. Standard treatment of pneumocepha...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2009.02.012
更新日期：2010-07-01 00:00:00
abstract：OBJECTIVES:To evaluate the efficacy of automatic benign paroxysmal positional vertigo (BPPV) diagnosis and treatment system for BPPV compared with the manual repositioning group. METHODS:Two hundred thirty patients diagnosed as idiopathic BPPV who were admitted from August 2018 to July 2019 in Zhejiang Hospital were i...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102412
更新日期：2020-01-01 00:00:00
abstract：:The relation between noise-induced permanent threshold shift (NIPTS) and vibration-induced dysfunction in the digital circulation was examined in a longitudinal survey among forest workers. The survey was based on annual examinations done between 1972 and 1983. Thirty-two forest workers with digital vasospasms were co...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(86)80013-8
更新日期：1986-11-01 00:00:00
abstract：OBJECTIVES:A case of pediatric otogenic lateral sinus thrombosis is reported, followed by a substantive literature review. DESIGN:104 patients were reviewed, culled from published case reports from 1993 to 2011 on the PubMed database. METHODS:All full text case reports on the PubMed database from 1993 to 2011 with pa...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2012.09.011
更新日期：2013-03-01 00:00:00
abstract：:A new mutation of the inner ear was discovered in golden hamsters raised in our laboratories. Although scanning electron microscopy showed a normal arrangement of individual stereocilia on the first row of outer hair cells, the entire bundle of stereocilia were irregular in orientation and scattered in several directi...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(88)80031-0
更新日期：1988-09-01 00:00:00
abstract：PURPOSE:Hard cochlear implant failures are diagnosed by objective tests whereas soft failures are suspected on the basis of clinical signs and symptoms. This study reviews our experience with children in tertiary pediatric medical center who underwent revision cochlear implantation, with emphasis on soft failures. MAT...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2017.12.014
更新日期：2018-01-01 00:00:00
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journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2003.09.007
更新日期：2004-03-01 00:00:00
abstract：OBJECTIVE:The objective of the study was to review the diagnosis and treatment of primary immunodeficiency disease (PID) and the role of otolaryngologists in the management of PID. METHODS:A search was conducted of PubMed and the Web sites of organizations for PID patients for literature pertaining to the diagnosis an...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
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更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to determine the immediate effects of intraperitoneal doses of gentamicin (GM) which would result in variable degrees of destruction of crista ampullary hair cells of frogs. This information will serve as a baseline guide to cell regeneration experiments on the damaged vestibular sens...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2004.08.007
更新日期：2005-03-01 00:00:00
abstract：PURPOSE:To investigate the activities of inner ear melanin in patients with pigment variations and disorders. Our purpose was to find evidence on the effects of melanin-containing cells by measuring the high-frequency threshold and the latency of stapes reflex in patients with vitiligo. PATIENTS AND METHODS:Twenty-nin...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(98)90038-2
更新日期：1998-11-01 00:00:00
abstract：INTRODUCTION:US Food and Drug Administration (USFDA) has licensed four Haemophilus influenzae type B (Hib) vaccines for use in children. Haemophilus influenzae type B is by far the most common pathogen in childhood epiglottitis and it is hoped that with the introduction of the Hib vaccine that a corresponding decrease ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/0196-0709(94)90085-x
更新日期：1994-11-01 00:00:00
abstract：PURPOSE:The purpose to this study is to identify correlations between pathology and dynamic contrast-enhanced magnetic resonance imaging (MRI) and to assess the utility of this technique in the evaluation of radiation response for head and neck cancer. MATERIALS AND METHODS:MRI was prospectively performed after radiot...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2004.11.011
更新日期：2005-05-01 00:00:00
abstract：:We aimed to investigate the relationship between peripheral vertigo and inflammation by using the neutrophil-to-lymphocyte ratio (NLR) as an inflammatory marker. We recruited 103 patients with peripheral vertigo (71 women, 32 men; mean age, 39.8 ± 14.7 years) who presented to the Otolaryngology Department of Dumlupina...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2014.08.004
更新日期：2014-11-01 00:00:00
abstract：BACKGROUND:Primary hyperparathyroidism is primarily caused by parathyroid adenoma, followed by hyperplasia and parathyroid carcinoma. In the era of minimally invasive, targeted parathyroidectomy, the main challenge remains that of distinguishing intraoperatively pathological parathyroid from normal glands and peri-thyr...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102558
更新日期：2020-01-01 00:00:00
abstract：PURPOSE:The standard treatment for patients with early glottic carcinoma in Israel has been radiotherapy. In recent years, encouraging results with laryngo-microscopic carbon dioxide laser surgery as a treatment for early glottic carcinoma has changed our treatment strategy. We conducted a retrospective study to invest...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1053/ajot.2003.2
更新日期：2003-01-01 00:00:00
abstract：PURPOSE:To assess the effectiveness of Buteyko breathing technique in patients with obstructive Eustachian tube dysfunction (ETD). MATERIALS AND METHODS:Fifty-one patients (77 ears) aged between 21 and 62 years were randomized to Buteyko breathing in conjunction with medical management (nasal steroid) group or medical...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,随机对照试验
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更新日期：2019-01-01 00:00:00
abstract：PURPOSE:The aim of this article is to present the development of brown tumors in the jaws as a definite feature of hyperparathyroidism (HPT), whether primary or secondary. PATIENTS AND METHODS:The study included 2 cases of primary HPT and 3 cases of secondary HPT associated with brown tumors in the jaws. The patients ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
doi：10.1016/j.amjoto.2005.11.004
更新日期：2006-07-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to report 2 cases of extramedullary plasmacytoma (EP) with localization to middle ear that were diagnosed and managed in our department. METHODS:The first patient was a 60-year-old man with progressive hearing loss, tinnitus, recurrent episodes of otalgia, and otorrhea in his right e...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
doi：10.1016/j.amjoto.2008.07.004
更新日期：2009-09-01 00:00:00
abstract：PURPOSE:Little is known about prognostic factors and treatment outcomes in young patients with anaplastic thyroid cancer (ATC). The goal of this study is to define the clinical features of this subgroup. MATERIAL AND METHODS:Patients age 55 or younger with either ATC or well-differentiated thyroid cancer (WDTC) with a...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2013.07.008
更新日期：2013-11-01 00:00:00
abstract：:Semicircular canal dehiscence (SCD) is a condition of the inner ear wherein the temporal bone adjacent to the vestibular apparatus thins or erodes completely. It has no clear epidemiological predisposing factors that have been identified to date. Its diagnosis is made difficult by a clinical presentation that often ov...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2014.08.012
更新日期：2015-01-01 00:00:00
abstract：:Merkel cell carcinoma is a rare malignant tumor of the skin. The face and extremities are the most common locations. We present a case of Merkel cell carcinoma of the auricle in a 93-year-old man subsequently treated with surgery and radiotherapy. The clinical features, prognosis, and treatment of the Merkel cell carc...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2005.01.013
更新日期：2005-09-01 00:00:00
abstract：:Sarcoidosis is a systemic chronic granulomatous disease of unknown etiology. Although it most commonly affects African-Americans, Scandinavians, and the Irish, individuals of all races and ethnicities are susceptible. The otolaryngologist will most frequently encounter sarcoidosis involving the sinonasal region; howev...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1053/ajot.2002.28783
更新日期：2002-01-01 00:00:00
abstract：BACKGROUND:The goals of successful reconstructive surgery are to restore function and cosmesis; however, limitation of resources can become an important consideration in low-middle income countries. METHODS:We describe our experience using the submental island flap in two cases during a short-term surgical camp in Eas...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2016.09.012
更新日期：2017-01-01 00:00:00
abstract：PURPOSE:In this study, we aimed to determine the function of the cochlea and peripheral and central auditory pathways with migraine. MATERIALS AND METHODS:Fifty-eight patients with migraine and 40 healthy subjects were assessed using routine diagnostic audiometry along with transient evoked otoacoustic emissions (TOAE...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2011.10.008
更新日期：2012-07-01 00:00:00
abstract：:Auditory middle latency responses (latencies 6 to 50 msec in guinea pigs) were recorded from eight awake, restrained guinea pigs. Before recording, screw electrodes were implanted in the skull in a coronal plane in line with the bregma. Another electrode, which served to monitor auditory brainstem responses, was place...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(83)80013-1
更新日期：1983-03-01 00:00:00
abstract：PURPOSE:Determine the clinical efficacy of comprehensive neurotologic testing in patients presenting with complaints of hearing loss, tinnitus and/or dizziness. METHODS:This is a retrospective analysis of 1170 consecutive charts of patients who presented between 1980 and 2013 with neurotologic complaints. Demographic ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2018.09.009
更新日期：2019-01-01 00:00:00
abstract：:Four patients had Eikenella corrodens infections involving the head and neck in different ways. The organism is discussed as a pathogen in mixed infections and as the predominant organism in other processes. As part of the resident microflora of mucous membrane surfaces, it is an important potential pathogen in a numb...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(86)80018-7
更新日期：1986-11-01 00:00:00
