Abstract：
BACKGROUND:Estrogen receptor (ER)-positive breast carcinomas possess a less aggressive phenotype than ER-negative breast carcinomas. We hypothesize that a set of genes exists that is expressed only in ER-negative breast carcinomas, which account for the more malignant phenotypic characteristics of these tumors. METHODS:We have used a new technique of polymerase chain reaction select suppression subtractive hybridization to identify genes that are expressed only in ER-negative carcinomas. RESULTS:Seventy-one cDNA clones generated by suppression subtractive hybridization were screened by Northern blot analysis with RNA from ER-positive MCF7 and ER-negative MDA-MB-231 breast carcinoma cell lines. Fifteen clones were differentially expressed in MDA-MB-231 cells. Five of these 15 clones were consistently found to be associated with the ER-negative phenotype in a panel of eight breast carcinoma cell lines. Sequence analysis demonstrated that three of these clones were derived from vimentin and two clones from moesin. Western blot analysis with antihuman moesin antibody confirmed that moesin protein was overexpressed in ER-negative breast carcinoma cell lines but absent from ER-positive breast carcinomas. Moesin mRNA was examined in a panel of 29 primary breast carcinomas with semi-quantitative reverse transcriptase-polymerase chain reaction. Moesin expression was found to be decreased significantly in ER-positive compared with ER-negative tumors (P < .01). CONCLUSIONS:Vimentin and moesin are differentially expressed in association with the ER-negative breast cancer phenotype. Moesin is a membrane/actin filament protein involved in dynamic restructuring of the cell surface and filopodia, a cell structure needed for cell adhesion and motility. Moesin may play a role in the invasiveness and pattern of metastasis characteristic of ER-negative breast cancers.
journal_name
Surgeryjournal_title
Surgeryauthors
Carmeci C,Thompson DA,Kuang WW,Lightdale N,Furthmayr H,Weigel RJsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
211-7issue
2eissn
0039-6060issn
1532-7361pii
S0039606098002232journal_volume
124pub_type
杂志文章相关文献
SURGERY文献大全abstract：BACKGROUND:Tumor necrosis factor (TNF) is rapidly gaining recognition as one of the early, critical mediators of several inflammatory conditions, most notably endotoxic shock. The purposes of this study were to determine whether TNF levels are raised in severe acute pancreatitis, thus pointing to its role as a potentia...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：BACKGROUND:Improvement of angina pectoris symptoms after cholesterol lowering has raised questions as to the underlying mechanisms. METHODS:Rabbit experiment: We compared arterial blood samples from New Zealand White cholesterol-supplemented rabbits (n = 6) with nonsupplemented rabbit samples (n = 4) in a closed-loop ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1067/msy.1998.90944
更新日期：1998-10-01 00:00:00
abstract：BACKGROUND:Although prophylactic glucocorticoids have been used before liver resection to minimize liver dysfunction, it is unknown whether treatment with glucocorticoids will accelerates recovery from hyperbilirubinemia after liver resection. METHODS:In this open-label, randomized, controlled trial, patients with hyp...
journal_title：Surgery
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.surg.2018.09.002
更新日期：2019-03-01 00:00:00
abstract：:An unusual case of Mondor's disease associated with carcinoma of the breast is reported. "Forked string" and "triple beads" have been reported previously in this disease. In this patient, however, one of the "beads" of her triple beads was malignant. It is suggested that biopsy and histologic examination are the best ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：BACKGROUND:Pediatric umbilical hernia repair is a common procedure that requires minimal tissue disruption. We examined variation in opioid prescription fills after repair of uncomplicated umbilical hernias to characterize the types and doses of medication used and persistent postsurgical use. METHODS:Using the Truven...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2018.10.029
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:Lateral neck lymph node (LN) metastases (N1b) have been identified as independent risk factors of recurrence in patients with papillary thyroid carcinoma (PTC). OBJECTIVE:This study aimed to determine the predictive factors of recurrence in N1b PTC patients and to clarify the postoperative event patterns. ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2015.08.033
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:Medullary carcinoma of the thyroid gland (MCT) is a disease commonly associated with regional metastases. Apart from surgical resection, there are limited therapeutic options for such patients. Given the variable and often prolonged survival of such patients, the benefit of surgical resection is difficult to...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：BACKGROUND:This study analyzes trends in hospitalization and outcome for adult, elderly, and pediatric trauma victims in the Florida Trauma System (FTS) from 1991 to 2003, during which time the number of centers nearly doubled from 11 to 20. METHODS:Administrative data was queried for all admissions with at least one ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2006.01.012
更新日期：2006-07-01 00:00:00
abstract：BACKGROUND:The impact of the location of colorectal cancer on patient outcomes has been reported in several settings. The objective of this study was to assess the prognostic impact of the location of the primary colon cancer among patients with colorectal cancer peritoneal metastases undergoing complete cytoreductive ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2018.07.027
更新日期：2019-02-01 00:00:00
abstract：:The central venous dialysis catheter has gained wide acceptance for short-term hemodialysis with the realization of the need to spare peripheral extremity vessels for the creation of more durable internal arteriovenous fistulas. The Hemocath (Quinton Instrument Co., Seattle, Wash.), a soft, silicone rubber, double-lum...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:Hypertriglyceridemia commonly accompanies clinical sepsis and may be caused by increased hepatic production or decreased clearance of triglyceride from the bloodstream. In contrast, enhanced lipid clearing capacity is usually seen after uncomplicated trauma. The purpose of the study was to determine the role of lipopr...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：BACKGROUND:Pheochromocytoma (Pheo) is usually considered a sporadic disease. Recently, an increasing rate of genetically based tumors has been reported. However, the need for systematic screening of unsuspected germline mutations in apparently sporadic forms is still debated. This study aimed to assess the effective ra...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2011.09.024
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND:This study was undertaken to determine the true incidence of renal impairment after aortic surgery and its association with intraoperative fluctuations in renal artery blood flow and cardiac output. METHODS:Direct measurements of renal artery blood flow were undertaken with electromagnetic flowmetry in 19 p...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：INTRODUCTION:End-stage renal disease (ESRD) is associated with poor medical outcomes. This study aimed to compare early outcomes of elective and emergency operation for diverticulitis among older adults with and without a diagnosis of ESRD. METHODS:Patients 65 years of age and older with a primary diagnosis of diverti...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2014.03.034
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND:These experiments were designed to quantitatively compare the patterns of tissue thrombosis, cytokine response, and tissue viability in a murine model of partial (PI) versus complete hindlimb ischemia (CI), alone or with reperfusion (RE). METHODS:The control tension tourniquet was used to establish either P...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2005.06.005
更新日期：2005-08-01 00:00:00
abstract：:An expandable intraluminal graft mounted coaxially over an angioplasty balloon catheter was used in dog arteries. The graft, a wire mesh tube that has the ability to retain its expanded shape, opposes elastic recoil of the arterial wall after maximum balloon inflation. Eighteen grafts were placed in the abdominal aort...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:A series of 76 patients treated for carcinoma of the extrahepatic bile ducts at three Swedish hospitals during the years 1952 to 1973 was studied. The mean age of the patients was 68 years. Gallstones were found in 22 patients, whereas 11 patients had undergone cholecystectomy earlier for gallstone disease. At operati...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：BACKGROUND:Activated nuclear factor-kappaB (NF-kappaB) is associated reportedly with the pathogenesis of numerous malignancies. This study investigated whether a common insertion (ins)/deletion (del) polymorphism (-94 ins/del ATTG) in the NFKB1 promoter is associated with susceptibility to gastric cancer and its tumor ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2008.11.001
更新日期：2009-03-01 00:00:00
abstract：BACKGROUND:Outcome for most abdominal wall defects is related to the presence or absence of additional anomalies or prematurity. In gastroschisis, outcome is almost as closely related to the severity of the inflammatory "peel" on bowel that is thought to result from direct contact with amniotic fluid. Improving eviscer...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:Effective esophageal peristalsis is a major determinant of esophageal clearance function and may contribute to the development of complications in gastroesophageal reflux disease. Using 24-hour ambulatory esophageal manometry, we compared the circadian esophageal motor activity of normal volunteers to that of patients...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：BACKGROUND:Hypoparathyroidism is the most frequent permanent complication of thyroid surgery. Our hypothesis is that human precursor cells in culture can be differentiated into parathyroid cells and used to reconstitute function. Human embryonic stem cells (hESCs) are a stable model to study differentiation into parath...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2010.09.021
更新日期：2010-12-01 00:00:00
abstract：:A case of pneumatosis cytoides intestinalis associated with jejunoileal bypass is reported. The association of increased colonic gas, secondary to fermentation of undigested lactose in the colon, and the reflux of the excessive gas into the excluded segment of small bowel and dissection of the gas into the bowel wall ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：BACKGROUND:As a measure of quality, ambulatory surgery centers have begun reporting rates of hospital transfer at discharge. This process, however, may underestimate the acute care needs of patients after care. We conducted this study to determine rates and evaluate variation in hospital transfer and hospital-based, ac...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2013.12.008
更新日期：2014-05-01 00:00:00
abstract：BACKGROUND:Our aim was to identify differential expression of genes in hepatocellular carcinoma (HCC) with the ultimate goal of discovering novel diagnostic and therapeutic targets. METHODS:We examined differences in gene expression between HCC and noncancerous liver tissue using a cDNA array with probes for 15,843 ge...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2010.12.007
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND:Carotid endarterectomy (CEA) reduces the risk of stroke in selected patients with symptomatic and asymptomatic carotid disease, but its beneficial influence on cognitive performance in the elderly remains debatable. This prospective study sought to determine early and long-term neurocognitive outcomes after ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2011.06.031
更新日期：2012-01-01 00:00:00
abstract：:Augmentation of cutaneous blood flow by postganglionic lumbar sympathectomy may not reflect an increase in nutritive vascular supply to the dermal tissues. Transcutaneous oxygen tension (TcPO2) was compared with radionuclide microsphere determination of dermal microcirculation in a hind limb sympathectomy model in 20 ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：OBJECTIVES:Outpatient thyroid surgery is becoming increasingly common. The aim of this study was to clarify the principles for safe outpatient thyroid surgery and review our outcomes with the use of a protocol for outpatient thyroidectomy in a large patient cohort. STUDY DESIGN:A systematic analysis of a prospectively...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2015.08.007
更新日期：2016-02-01 00:00:00
abstract：BACKGROUND:Sclerotherapy is usually effective in controlling acutely bleeding esophageal varices. It may not be as effective as shunt surgery for prevention of rebleeding; therefore we undertook a prospective study comparing interposition mesocaval shunt (MCS) and repeated sclerotherapy. METHODS:Forty-five patients (m...
journal_title：Surgery
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0039-6060(05)80248-x
更新日期：1995-05-01 00:00:00
abstract：:Planned excisions of tumors involving the abdominal or chest wall should not be compromised because of lack of tissue for wound closure. Reconstruction with an inert polypropylene mesh provides a strong and stable covering for the defect. Mobilization of adjacent skin flaps to provide covering for the mesh is desirabl...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1975-10-01 00:00:00
abstract：:Serious complications of local venous hypertension occur rarely in the upper extremity. These are most frequently the result of a dysfunctional arteriovenous fistula and may produce changes indistinguishable from those of chronic lower-extremity venous insufficiency. A combination of arterial and venous contrast imagi...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
