Abstract:
:Four different marine cyanobacterial morphotypes were tested for their efficacy to produce siderophores in Fe minus [Fe(-)], Fe minus Uranium dosed [Fe(-)U(+)], and Fe dosed Uranium dosed [Fe(-)U(+)] media. Of the four organisms tested, Synechococcus elongatus BDU 130911 produced the highest amount of siderophore of 58μgmg(-1) dryweight. The results clearly indicate that uranium induces siderophore production in marine cyanobacteria even in the presence of iron [Fe(-)U(+)] condition. The type of siderophore revealed by FeCl(3), Tetrazolium and Atkin's tests is a hydroxamate; and thin layer chromatogram also authenticates our finding. Uranium siderophore complexation was confirmed through modified Chrome Azurol S (CAS) assay as well as based on residual uranium presence. In silico docking studies further validate siderophore complexation with uranium.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Rashmi V,Shylajanaciyar M,Rajalakshmi R,D'Souza SF,Prabaharan D,Uma Ldoi
10.1016/j.biortech.2012.12.016subject
Has Abstractpub_date
2013-02-01 00:00:00pages
204-10eissn
0960-8524issn
1873-2976pii
S0960-8524(12)01871-8journal_volume
130pub_type
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