Abstract:
:Kudoa septempunctata, a myxosporean parasite, was recently identified as the causative agent of food poisoning resulting from the consumption of raw olive flounder (Paralichthys olivaceus). A single blind inter-laboratory study, involving 5 laboratories, was conducted to validate a quantitative real-time PCR assay for the detection of the parasite. We obtained relatively constant values for log rDNA copies/g from these laboratory analyses (SD = 0.35-0.86), suggesting the validity of the real-time PCR method for the detection of K. septempunctata in P. olivaceus. Detection of K. septempunctata in muscle tissue samples collected from both sides of the fish indicated that K. septempunctata infection spreads throughout the body of P. olivaceus. K. septempunctata infection in P. olivaceus is thought to occur during the early stage of fish growth because a K. septempunctata gene was detected in 1 of 300 P. olivaceus fry tested. Feeds seem not to be sources of infection. To prevent food poisoning due to K. septempunctata, the mechanism of infection and proliferation of K. septempunctata in P. olivaceus should be elucidated, and other hosts of the parasite should be identified. The sensitive real-time PCR method described here will be a useful tool for resolving these issues.
journal_name
Jpn J Infect Disjournal_title
Japanese journal of infectious diseasesauthors
Iijima Y,Nakanishi N,Furusawa H,Ohnishi T,Sugita-Konishi Ydoi
10.7883/yoken.65.436subject
Has Abstractpub_date
2012-01-01 00:00:00pages
436-8issue
5eissn
1344-6304issn
1884-2836journal_volume
65pub_type
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