Abstract:
PREMISE OF THE STUDY:New microsatellite primers were developed for testing genetic differentiation within Nothoceros aenigmaticus and their potential use in other Nothoceros species. The microsatellites are designed to investigate partitioning of genetic variation in a taxon with a peculiar sex allopatry in the southern Appalachian Mountains and relationships with conspecific sexual populations from Mexico. METHODS AND RESULTS:We used two methods for microsatellite development: an enriched library and second-generation shotgun sequence reads. From these two methods, a total of nine primer pairs were selected and tested on 89 southern Appalachian N. aenigmaticus accessions, nine Mexican accessions, and 16 N. vincentianus accessions. Three mitochondrial loci were recovered from the enriched library method and six loci from 454 shotgun sequencing: three were from the chloroplast and three from the nucleus. The primers amplified repeats with two to 20 alleles per locus. CONCLUSIONS:New microsatellite primers were developed for testing genetic differentiation within N. aenigmaticus and potentially for use in other Nothoceros species. We present one of the first reports of highly polymorphic mitochondrial microsatellites in plants.
journal_name
Am J Botjournal_title
American journal of botanyauthors
Villarreal JC,Forrest LL,McFarland K,Goffinet Bdoi
10.3732/ajb.1100392subject
Has Abstractpub_date
2012-03-01 00:00:00pages
e88-90issue
3eissn
0002-9122issn
1537-2197pii
ajb.1100392journal_volume
99pub_type
杂志文章abstract:PREMISE OF THE STUDY:Microsatellites were developed for the native, perennial herbs Houstonia longifolia and H. purpurea to establish rates and patterns of hybridization among these and other closely related lineages within the genus. METHODS AND RESULTS:Seven primer sets were developed and tested using populations ac...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1000294
更新日期:2011-02-01 00:00:00
abstract::Pedilanthus coalcomanensis was described from specimens collected by George B. Hinton in 1941 but was not collected again until 1999, when we found it in a tropical deciduous forest near Tehuantepec, in Chinicuila, Michoacán, Mexico. After analyzing Hinton's original collection notes, we concluded that this is the typ...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.89.9.1485
更新日期:2002-09-01 00:00:00
abstract::Breakdown of genetically enforced self-incompatibility (SI), an extremely common and important evolutionary transition in plants, has conventionally been conceived as a qualitative rather than a quantitative change. We evaluated qualitative and quantitative variation in SI for four populations of Witheringia solanacea...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.93.4.592
更新日期:2006-04-01 00:00:00
abstract::Reproductive traits of tropical tree species vary predictably in relation to successional stage, but this variation may be due to the species' phylogenetic histories rather than selective pressures imposed by regeneration requirements. Reproductive phenology, tree size at the onset of reproduction, and fecundity of 11...
journal_title:American journal of botany
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract:UNLABELLED:• PREMISE OF THE STUDY:Contact zones between diploids and their autopolyploid descendants represent a unique evolutionary venue for studying polyploid establishment, cytotype coexistence, and interactions. Here, we examine cytotype coexistence in a diploid-tetraploid contact zone of a perennial herb, Cardam...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1500052
更新日期:2015-08-01 00:00:00
abstract::The geographic distribution of diploid and tetraploid cytotypes and cpDNA haplotypes throughout the entire range of Aucuba japonica was investigated. We measured relative genome size using flow cytometry and sequenced two cpDNA intergenic regions, atpB-rbcL and psbA-trnH (GUG). Two haplotypes include both diploids and...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.90.11.1645
更新日期:2003-11-01 00:00:00
abstract:UNLABELLED: PREMISE OF THE STUDY:A mixture of outcrossing and selfing is often observed in plant populations. Although mixed mating is ubiquitous, it has several potential evolutionary explanations. Mixed mating may be actively maintained by selection, passively determined by the pollination environment, or a transiti...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1000032
更新日期:2010-06-01 00:00:00
abstract:PREMISE OF THE STUDY:Direct tests of a species distribution model (SDM) were used to evaluate the hypothesis that the northern and southern edges of Mimulus bicolor's geographical range are limited by temperature and precipitation. METHODS:Climatic suitability was predicted using an SDM informed only by temperature an...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1600414
更新日期:2017-06-01 00:00:00
abstract:PREMISE OF THE STUDY:Bio-ontologies are essential tools for accessing and analyzing the rapidly growing pool of plant genomic and phenomic data. Ontologies provide structured vocabularies to support consistent aggregation of data and a semantic framework for automated analyses and reasoning. They are a key component of...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1200222
更新日期:2012-08-01 00:00:00
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journal_title:American journal of botany
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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journal_title:American journal of botany
pub_type: 杂志文章
doi:10.1002/ajb2.1153
更新日期:2018-09-01 00:00:00
abstract::Five plant communities in Western Australia, as well as selected desert and Rocky Mountain species of the western USA, were surveyed to evaluate associations among leaf structure, orientational properties, and the sunlight exposure and precipitation characteristic of each community. Selected leaf structural features h...
journal_title:American journal of botany
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:PREMISE:The Eocene-Oligocene transition (EOT; 34-33 Ma) was marked by global cooling and increased seasonality and aridity, leading to a shift in North American floras from subtropical forests to deciduous hardwood forests similar to today. This shift is well documented taxonomically and biogeographically, but its ecol...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.1002/ajb2.1580
更新日期:2020-12-01 00:00:00
abstract::Several alpine species have outlying populations in the lowlands and lower mountains north of the Alps. These small, isolated populations are usually described as either (1) glacial relics, (2) descendants from populations living on forelands and moraines during the ice ages, or (3) populations founded by long-distanc...
journal_title:American journal of botany
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::The biogeography of plant-animal interactions is a novel topic on which many disciplines converge (e.g., reproductive biology, biogeography, and evolutionary biology). Narrative reviews have indicated that tropical columnar cacti and agaves have highly specialized pollination systems, while extratropical species have ...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.0800392
更新日期:2009-10-01 00:00:00
abstract:PREMISE OF THE STUDY:Chloroplast microsatellites were developed in Theobroma cacao to examine the genetic diversity of cacao cultivars in Trinidad and Tobago. METHODS AND RESULTS:Nine polymorphic microsatellites were designed from the chloroplast genomes of two T. cacao accessions. These microsatellites were tested in...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1100306
更新日期:2011-12-01 00:00:00
abstract:PREMISE OF THE STUDY:Ferns and lycophytes are the only extant land plants with two free-living generations (sporophytes and gametophytes); hence, a single species may have two different distributions. The distribution of the gametophytes of most fern species, which are much smaller in size than sporophytes, are almost ...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1200555
更新日期:2013-04-01 00:00:00
abstract::The vegetative cycle of the foliicolous lichen Phyllophiale, from propagule germination to propagule production, was studied by light microscope observation of thalli colonizing plastic cover slips placed within a lowland tropical forest. Discoid propagules germinated by growth of radially arranged fungal cells and de...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.89.11.1741
更新日期:2002-11-01 00:00:00
abstract::Phylogenetic relationships of 38 species of the Alibertia group (Rubiaceae) and two outgroup species were investigated using the nuclear ribosomal 5S nontranscribed spacer (5S-NTS) and the internal transcribed spacers (ITS). Analysis of the data sets separately and in combination resulted in several well-supported and...
journal_title:American journal of botany
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::The pollination biology of the cactus Pachycereus pecten-aboriginum was studied in a tropical location in western Mexico (ca. 18° N latitude) to compare with data from a northern population (ca. 28° N latitude). Throughout this range, the nectar-feeding bat Leptonycteris curasoae is resident within the tropics but mig...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.91.6.850
更新日期:2004-06-01 00:00:00
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journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.0900063
更新日期:2009-08-01 00:00:00
abstract::Chrysothamnus nauseosus (Asteraceae) is a complex polymorphic shrub species widely distributed in western North America. In a study of 86 populations belonging to 15 subspecies, achene mass varied among populations over a sevenfold range. Achene mass was closely correlated with capitulum size at the subspecies level, ...
journal_title:American journal of botany
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract:PREMISE OF THE STUDY:Microsatellite primers were developed in the traditional Tibetan medicinal plant Halenia elliptica D. Don to investigate its genetic diversity and population genetic structure. METHODS AND RESULTS:Using the Fast Isolation by AFLP of Sequences Containing (FIASCO) repeats protocol, 24 primer sets we...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1000534
更新日期:2011-07-01 00:00:00
abstract::The temperature dependence of freezing-induced xylem cavitation was studied in a Chihuahuan desert population of Larrea tridentata (Zygophyllaceae). Field measurements of wood temperature and xylem embolism were combined with anatomical studies and laboratory measurements of embolism in stem and root samples frozen un...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.89.12.1916
更新日期:2002-12-01 00:00:00
abstract:PREMISE OF THE STUDY:Conifers are an important living seed plant lineage with an extensive fossil record spanning more than 300 million years. The group therefore provides an excellent opportunity to explore congruence and conflict between dated molecular phylogenies and the fossil record. METHODS:We surveyed the curr...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.1002/ajb2.1143
更新日期:2018-09-01 00:00:00
abstract:PREMISE OF THE STUDY:Productivity in drylands may depend on the sensitivity of interactions between plants and biocrusts. Given future climate variability, it is essential to understand how interactions may be context-dependent with precipitation regime. Furthermore, little is known about the additional interactions of...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.1002/ajb2.1120
更新日期:2018-07-01 00:00:00
abstract:PREMISE OF THE STUDY:High intraspecific cytogenetic variation in the sedge genus Carex (Cyperaceae) is hypothesized to be due to the "diffuse" or non-localized centromeres, which facilitate chromosome fission and fusion. If chromosome number changes are dominated by fission and fusion, then chromosome evolution will re...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1000046
更新日期:2011-01-01 00:00:00
abstract:UNLABELLED: PREMISE OF THE STUDY:Verbenaceae consist of trees, shrubs, lianas, and herbs distributed primarily in Latin America, where they occur in a wide array of ecosystems. A second center of diversity exists in Africa. Competing morphology-based classifications that rely on different traits conflict in significan...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1000144
更新日期:2010-10-01 00:00:00
abstract::The evolutionary origins of the morphological and taxonomic diversity of angiosperms is poorly known. We used the genus Melianthus to explore the diversification of the southern African flora. Melianthus comprises eight species, and a phylogeny based on one nuclear and two plastid genes, as well as a morphological dat...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.93.7.1052
更新日期:2006-07-01 00:00:00
abstract:UNLABELLED: PREMISE OF THE STUDY:Fuchsia (Onagraceae) anthers, pollen, and an ornithophilous Fuchsia-like flower from an earliest Miocene lacustrine diatomite deposit at Foulden Maar, southern New Zealand confirm a long record for Fuchsia in New Zealand and probably an equally long history for its distinctive honeyeat...
journal_title:American journal of botany
pub_type: 杂志文章
doi:10.3732/ajb.1200643
更新日期:2013-10-01 00:00:00