Critical cellulase and hemicellulase activities for hydrolysis of ionic liquid pretreated biomass.

Abstract:

:Critical cellulase and hemicellulase activities are identified for hydrolysis of ionic liquid (IL) pretreated poplar and switchgrass; hemicellulase rich substrates with largely amorphous cellulose. Enzymes from Aspergillus nidulans were expressed and purified: an endoglucanase (EG) a cellobiohydrolase (CBH), an endoxylanase (EX) and an acetylxylan esterase (AXE). β-Xylosidase (βX) from Selenomonas ruminantium and a commercial β-glucosidase (βG) from Novozyme 188 were admixed with the A. nidulans enzymes. Statistical analysis indicates that βG and βX activities are significant for both glucose and xylose yields for the two substrates. EG is a significant factor for glucan hydrolysis while EX is significant for xylan hydrolysis of the substrates. The CBH, which has activity on crystalline cellulose and negligible activity on amorphous cellulose, was not a significant factor in glucan hydrolysis. EX is significant in glucan hydrolysis for poplar. The addition of AXE significantly improves xylan hydrolysis for poplar but not switchgrass.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Barr CJ,Mertens JA,Schall CA

doi

10.1016/j.biortech.2011.10.101

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

480-5

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(11)01590-2

journal_volume

104

pub_type

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