Abstract:
:Gene therapy is an attractive method for the treatment of cardiovascular disease. However, using current strategies, induction of gene expression at therapeutic levels is often inefficient. In this study, we show a novel electroporation (EP) method to enhance the delivery of a plasmid expressing an angiogenic growth factor (vascular endothelial growth factor, VEGF), which is a molecule previously documented to stimulate revascularization in coronary artery disease. DNA expression plasmids were delivered in vivo to the porcine heart with or without coadministered EP to determine the potential effect of electrically mediated delivery. The results showed that plasmid delivery through EP significantly increased cardiac expression of VEGF compared with injection of plasmid alone. This is the first report showing successful intracardiac delivery, through in vivo EP, of a protein expressing plasmid in a large animal.
journal_name
Gene Therjournal_title
Gene therapyauthors
Marshall WG Jr,Boone BA,Burgos JD,Gografe SI,Baldwin MK,Danielson ML,Larson MJ,Caretto DR,Cruz Y,Ferraro B,Heller LC,Ugen KE,Jaroszeski MJ,Heller Rdoi
10.1038/gt.2009.153subject
Has Abstractpub_date
2010-03-01 00:00:00pages
419-23issue
3eissn
0969-7128issn
1476-5462pii
gt2009153journal_volume
17pub_type
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