Abstract：
:Numerous approaches in gene therapy of human cancers are focused on the establishment of cell type specific or inducible expression vectors allowing the targeted and regulated expression of therapeutic genes. Various conditionally active vectors have been created carrying promoters responding to certain factors or therapeutic modalities (eg hormones, irradiation). The promoter of the multidrug resistance gene (mdr1) harbors such responsive elements and two of these elements have been related to drug responsiveness. In earlier studies we and others have characterized the mdr1 drug responsive-element in CAT reporter assays demonstrating its inducibility by MDR-associated drugs. To exploit this property, we linked the mdr1 promoter sequence to the human tumor necrosis factor alpha (TNF) cDNA in a retroviral vector and transduced the vector into human mammary and colon carcinoma cell lines. These cells were treated with various mdr1-associated drugs to induce TNF expression in vitro. We have shown that the mdr1 promoter-driven TNF expression is drug-inducible and that this induction is drug concentration and time dependent. The studies demonstrate the feasibility of the novel vector system for a chemotherapy-inducible expression of a chemosensitizing cytokine that is successful at enhancing cytotoxicity of drugs in cancer therapy.
journal_name
Gene Therjournal_title
Gene therapyauthors
Walther W,Wendt J,Stein Udoi
10.1038/sj.gt.3300451subject
Has Abstractpub_date
1997-06-01 00:00:00pages
544-52issue
6eissn
0969-7128issn
1476-5462journal_volume
4pub_type
杂志文章相关文献
GENE THERAPY文献大全abstract：:Gyrate atrophy (GA) of the choroid and retina is an autosomal recessive chorioretinal degeneration, caused by deficiency of the mitochondrial matrix enzyme ornithine-delta-aminotransferase (OAT). This deficiency results in the accumulation of ornithine in the body fluids and leads to hyperornithinemia. Although the cl...
journal_title：Gene therapy
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Multiple antitumor modalities may be necessary to overcome lung tumor-mediated immunosuppression and effectively treat non-small cell lung cancer (NSCLC). To evaluate a multimodality gene therapy approach for control of local tumor growth, a weakly immunogenic murine alveolar cell carcinoma, L1C2, was transduced with ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300531
更新日期：1997-12-01 00:00:00
abstract：:Radiosensitization of human gastrointestinal tumors by 5-fluorouracil (5-FU) has been studied in vitro and clinically in human cancer therapy trials. The bacterial enzyme cytosine deaminase (CD) converts the nontoxic prodrug 5-fluorocytosine (5-FC) into 5-FU. Human colon cancer cells stably expressing CD have been sho...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301196
更新日期：2000-06-01 00:00:00
abstract：:Varying degrees of metabolic abnormalities mediated by chronic inflammation are implicated in the chronic glomerular injuries associated with obesity. Interleukin (IL)-10, a pleiotropic cytokine, exerts anti-inflammatory effects in numerous biological settings. In the present study, we explored the biological benefits...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2011.183
更新日期：2012-05-01 00:00:00
abstract：:We have designed new vectors for the construction of recombinant adenoviruses containing expression cassettes in the E1 and/or E3 regions. Using a versatile set of restriction enzymes, the cassettes are cloned into small bacterial vectors and subsequently introduced into large plasmids containing the adenoviral sequen...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301047
更新日期：2000-01-01 00:00:00
abstract：:We introduce an injectable system for the formation of a biodegradable DNA-containing implant that releases DNA over a 2-month period to provide a robust and prolonged gene expression at the site. Sustained delivery of the appropriate plasmid DNA resulted in sustained expression of luciferase, the persistent appearanc...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301786
更新日期：2002-09-01 00:00:00
abstract：:Choroidal neovascularization (CNV) leads to loss of vision in age-related macular degeneration (AMD), the leading cause of blindness in adult population over 50 years old. In this study, we developed intravenously administered, nanoparticulate, targeted nonviral retinal gene delivery systems for the management of CNV....
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2008.185
更新日期：2009-05-01 00:00:00
abstract：:RNA interference (RNAi) is an evolutionarily conserved process by which plants and animals protect their genomes utilizing small, double-stranded RNAs to degrade target RNAs in a sequence-specific manner. Post-transcriptional gene silencing by these moieties can lead to degradation of both cellular and viral RNAs. It ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302339
更新日期：2004-12-01 00:00:00
abstract：:Alphavirus vectors based on Sindbis virus and Semliki Forest virus (SFV) were characterized as potential gene transfer vectors. Initial studies were performed using vectors engineered to transfer either lacZ or green fluorescent protein (GFP). High levels of gene transfer were achieved in human primary fibroblasts, BH...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301122
更新日期：2000-03-01 00:00:00
abstract：:A stable packaging cell line (Vero/BC-F) constitutively expressing fusion (F) protein of the human parainfluenza virus type 2 (hPIV2) was established for production of the F-defective and single round-infectious hPIV2 vector in a strategy for recombinant vaccine development. The F gene expression has not evoked cytost...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2014.55
更新日期：2014-08-01 00:00:00
abstract：:betaTC-tet cells are conditionally immortalized pancreatic beta cells which can confer long-term correction of hyperglycemia when transplanted in syngeneic streptozocin diabetic mice. The use of these cells for control of type I diabetes in humans will require their encapsulation and transplantation in non-native site...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300922
更新日期：1999-06-01 00:00:00
abstract：:We evaluated the efficacy of equine infectious anaemia virus (EIAV)-based lentiviral vectors encoding endostatin (EIAV.endostatin) or angiostatin (EIAV.angiostatin) in inhibiting angiogenesis and vascular hyperpermeability in the laser-induced model of choroidal neovascularisation (CNV). Equine infectious anaemia viru...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302769
更新日期：2006-08-01 00:00:00
abstract：:Fibrodysplasia ossificans progressiva (FOP) is an autosomal dominant congenital disorder characterized by progressive heterotopic bone formation. Currently, no definitive treatment exists for FOP. The activin receptor type IA / activin-like kinase 2 (ACVR1/ALK2) gene has been identified as the responsible gene for FOP...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2011.193
更新日期：2012-07-01 00:00:00
abstract：:Retroviral vectors have become the primary tool for gene delivery into hematopoietic cells, including T lymphocytes. Lentiviral vectors offer an advantage over Moloney murine leukemia virus (MuLV) vectors because of their ability to translocate across an intact nuclear membrane and integrate into the genome of nonprol...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301378
更新日期：2001-02-01 00:00:00
abstract：:The efficiency of plasmid gene transfer to skeletal muscle can be significantly improved by the application of an electrical field to the muscle following injection of plasmid DNA. However, this electrotransfer is associated with significant muscle damage which may result in substantial loss of transfected muscle fibr...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301522
更新日期：2001-08-01 00:00:00
abstract：:The prognosis of pancreatic adenocarcinoma is poor and current treatment ineffective. A novel treatment strategy is described here using a mouse model system for pancreatic cancer. Cells that have been genetically modified to express the cytochrome P450 2B1 enzyme are encapsulated in cellulose sulphate and implanted i...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300671
更新日期：1998-08-01 00:00:00
abstract：:Interferon-alpha (IFN-alpha) or CD80 transduction of tumor cells individually reduces tumorigenicity and enhances antitumor responses. Here, we report that the combination of IFN-alpha and CD80 cancer gene therapy in poorly immunogenic murine tumor models, the colorectal adenocarcinoma cell line MC38, and the methylch...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301034
更新日期：1999-12-01 00:00:00
abstract：:Cationic lipid-DNA complexes (lipoplexes) have been widely used as gene transfer vectors which avoid the adverse immunogenicity and potential for viraemia of viral vectors. With the long-term aim of gene transfer into skeletal muscle in vivo, we describe a direct in vitro comparison of two commercially available catio...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300604
更新日期：1998-04-01 00:00:00
abstract：:We have previously synthesized a new cationic liposome that displays high efficiency and low toxicity, 3 beta[l-ornithinamide-carbamoyl] cholesterol (O-Chol), using solid-phase synthesis. In this study, O-Chol was applied to in vitro and in vivo models of ovarian cancer. Intraperitoneal gene delivery for peritoneal di...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301704
更新日期：2002-07-01 00:00:00
abstract：:Gene targeting by single-stranded oligodeoxyribonucleotides (ssODNs) is a promising technique for introducing site-specific sequence alterations without affecting the genomic organization of the target locus. Here, we discuss the significant progress that has been made over the last 5 years in unraveling the mechanism...
journal_title：Gene therapy
pub_type： 杂志文章,评审
doi：10.1038/gt.2010.161
更新日期：2011-03-01 00:00:00
abstract：:We explored the possibility of using a genetic approach to inhibit integrin-mediated endothelial cell adhesion and survival. We constructed recombinant adenoviruses (Ads) expressing chimeric proteins consisting of the cytoplasmic and transmembrane domains of integrin beta1 (CH1), beta3 (CH3) or the beta1 transmembrane...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301236
更新日期：2000-08-01 00:00:00
abstract：:In view of our recent findings that a truncated form of the envelope (Env) glycoprotein of human immunodeficiency virus type 1 (HIV-1) was efficiently incorporated into MoMLV particles, we studied the generation of Moloney murine leukemia virus (MoMLV)/simian immunodeficiency virus (SIV) pseudotypes. Unlike HIV-1, bot...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300603
更新日期：1998-02-01 00:00:00
abstract：:Sustainable correction of severe human genetic disorders of self-renewing tissues, such as the blistering skin disease junctional epidermolysis bullosa (JEB), is facilitated by stable genomic integration of therapeutic genes into somatic tissue stem cells. While integrating viral vectors can achieve this, they suffer ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301978
更新日期：2003-07-01 00:00:00
abstract：:In vivo approaches to liver gene therapy will require restriction of transgene expression to hepatocytes. Since targeting of viral vectors exclusively to the liver is not easy to achieve, use of liver-specific promoters for driving expression of therapeutic genes is an interesting alternative. We have shown previously...
journal_title：Gene therapy
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:Self-amplifying RNA (saRNA) is a promising biotherapeutic tool that has been used as a vaccine against both infectious diseases and cancer. saRNA has been shown to induce protein expression for up to 60 days and elicit immune responses with lower dosing than messenger RNA (mRNA). Because saRNA is a large (~9500 nt), n...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/s41434-019-0095-2
更新日期：2019-09-01 00:00:00
abstract：:For patients with metastatic prostate cancer, the 5-year survival rate of 31% points to a need for novel therapies and improvement of existing modalities. We propose that p53 gene therapy and chemotherapy, when combined, will provide superior tumor cell killing for the treatment of prostate carcinoma. To this end, we ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/s41434-019-0071-x
更新日期：2020-02-01 00:00:00
abstract：:For the development of human immunodeficiency virus type 1 (HIV-1) vaccines, traditional approaches inducing virus-neutralizing antibodies have so far failed. Thus the effort is now focused on elicitation of cellular immunity. We are currently testing in clinical trials in the United Kingdom and East Africa a T-cell v...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302241
更新日期：2004-07-01 00:00:00
abstract：:In an earlier study exploring the potential of gene transfer to repair myocardial conduction defects, we observed that myotubes, generated by forced expression of MyoD, exhibit reduced excitability when also modified to express connexin43 (Cx43). We hypothesized that this effect was caused by gap junction-mediated cou...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302813
更新日期：2006-11-01 00:00:00
abstract：:The development of efficient systems for in vivo gene transfer to the central nervous system (CNS) may provide a useful therapeutic strategy for the alleviation of several neurological disorders. In this study, we evaluated the feasibility of nonviral gene therapy to the CNS mediated by cationic liposomes. We present ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302516
更新日期：2005-08-01 00:00:00
abstract：:Vessel wall inflammation and matrix destruction are critical to abdominal aortic aneurysm (AAA) formation and rupture. We have previously shown that urokinase plasminogen activator (uPA) is highly expressed in experimental AAA and is essential for AAA formation and expansion. In this study, we examined the effects of ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3303069
更新日期：2008-02-01 00:00:00
