Abstract:
:Gene targeting by single-stranded oligodeoxyribonucleotides (ssODNs) is a promising technique for introducing site-specific sequence alterations without affecting the genomic organization of the target locus. Here, we discuss the significant progress that has been made over the last 5 years in unraveling the mechanisms and reaction parameters underlying ssODN-mediated gene targeting. We will specifically focus on ssODN-mediated gene targeting in murine embryonic stem cells (ESCs) and the impact of the DNA mismatch repair (MMR) system on the targeting process. Implications of novel findings for routine application of ssODN-mediated gene targeting and challenges that need to be overcome for future therapeutic applications are highlighted.
journal_name
Gene Therjournal_title
Gene therapyauthors
Aarts M,te Riele Hdoi
10.1038/gt.2010.161subject
Has Abstractpub_date
2011-03-01 00:00:00pages
213-9issue
3eissn
0969-7128issn
1476-5462pii
gt2010161journal_volume
18pub_type
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