Abstract：
:We describe the application of a novel genome walking (GW) strategy for the one-shot identification of members of multigene families. The method was used to study the spinach Lhcb1 family (encoding the light harvesting complex protein Lhcb1), for which three cDNAs were known. Two additional genes and regulatory regions of the five members of the family were identified. For one of the newly detected genes, sequencing of full-length cDNA and analysis of regulatory regions by gel-shift are also reported. To our best knowledge, this is the first report on the use of a GW approach for the study of a multigene family.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Leoni C,Volpicella M,Placido A,Gallerani R,Ceci LRdoi
10.1016/j.jplph.2009.06.020subject
Has Abstractpub_date
2010-01-15 00:00:00pages
138-43issue
2eissn
0176-1617issn
1618-1328pii
S0176-1617(09)00333-2journal_volume
167pub_type
杂志文章abstract：:Mesophyll mitochondria from green leaves of the C(4) plants Zea mays (NADP-ME-type), Panicum miliaceum (NAD-ME-type) and Panicum maximum (PEP-CK-type) oxidized NADH, malate and succinate at relatively high rates with respiratory control, but glycine was not oxidized. Among the mitochondrial proteins involved in glycin...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.07.015
更新日期：2008-07-07 00:00:00
abstract：:The effect of hydroxyurea (HU) and amiprophos-methyl (APM) on the synchronization of suspension cultures of Satsuma mandarin (Citrus unshiu) and micronucleation of the suspension cells sequentially treated with both, HU and APM, were investigated. When suspension cultures in early-log phase were treated with 4 or 10mM...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.09.003
更新日期：2006-11-01 00:00:00
abstract：:Auxin is a well-known, crucial regulator of the entire plant lifecycle, not only orchestrating many aspects of plant growth and development, but also playing various roles in biotic and abiotic stress. This study reports the isolation and functional characterization of a DUF-966 domain-containing gene, At3g46110, re-n...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2019.152990
更新日期：2019-09-01 00:00:00
abstract：:Pandanus fascicularis is dioecious with the female plant producing a non-scented fruit while the male produces a flower rich in volatiles. The essential oil extracted from the flowers is economically exploited as a natural flavouring agent as well as for its therapeutic properties. Molecular dissection of this distinc...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.01.008
更新日期：2010-07-15 00:00:00
abstract：:Plant WRKY transcription factors play a vital role in abiotic stress tolerance and regulation of plant defense responses. This study examined AtWRKY11 and AtWRKY17 expression under ABA, salt, and osmotic stress at different developmental stages in Arabidopsis. We used reverse transcriptase PCR, quantitative real-time ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.04.007
更新日期：2018-07-01 00:00:00
abstract：:Although activation of A-type cyclin-dependent kinase (CDKA) is required for plant cell division, little is known about how CDKA is activated before commitment to cell division. Here, we show that auxin is required for the formation of active CDKA-associated complexes, promoting assembly of the complex in tobacco susp...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.01.005
更新日期：2007-09-01 00:00:00
abstract：:This article reports marked modulation of the activity and regulatory properties of phosphoenolpyruvate carboxylase (PEPC) by temperature and light in leaf discs as well as leaves of Amaranthus hypochondriacus. The activity of PEPC increased by 1.7-fold at 45 degrees C over 25 degrees C. Warm temperature also stimulat...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2007.09.003
更新日期：2008-07-07 00:00:00
abstract：:Climate change is submitting countries of the Mediterranean arc to periods of drought alternating with heavy rain and waterlogging. Eventual floods along with the rising CO2 in the atmosphere present an unpredictable scenario that affects crop survival. The effect of both stresses combined has been studied in sweet ch...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2017.03.011
更新日期：2017-06-01 00:00:00
abstract：:Protein metabolism and expression play important role in plant adaptation to water stress. The objectives of this study were to examine proteomic responses to water stress induced by polyethylene glycol (PEG) in creeping bentgrass (Agrostis stolonifera L.) leaves and to identify proteins associated with stress toleran...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.05.006
更新日期：2010-11-15 00:00:00
abstract：:The interactive effects of salinity and potassium deficiency on the growth, mineral elements and photosynthetic performance were investigated in wild (Hordeum maritimum L.) and cultivated barley (Hordeum vulgare L. var. Manel). At 28 d of growth, plants were treated with 3 mM K and 0 mM NaCl (3-0); 3 mM K and 100 mM N...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.06.013
更新日期：2009-12-15 00:00:00
abstract：:Syringyl lignins result from the oxidative polymerization of sinapyl alcohol in a reaction mediated by syringyl (basic) peroxidases. Several peroxidases have been identified in the genome of Arabidopsis thaliana as close homologues to ZePrx, the best characterized basic peroxidase so far, but none of these has been di...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2014.11.006
更新日期：2015-03-01 00:00:00
abstract：:The changes in some proteins involved in the light reactions of photosynthesis of the resurrection plant Haberlea rhodopensis were examined in connection with desiccation. Fully hydrated (control) and completely desiccated plants (relative water content (RWC) 6.5%) were used for thylakoid preparations. The chlorophyll...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.03.010
更新日期：2009-09-15 00:00:00
abstract：:A study of the effects of elevated levels of Cu2+ and Zn2+ on NO3- uptake and nitrate reductase (NR) activity in Scenedesmus sp. was carried out. The two metals inhibited NR and NO3- uptake in a concentration-dependent manner, with the latter process being inhibited more strongly than the former. After withdrawal of m...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-01238
更新日期：2004-05-01 00:00:00
abstract：:Glycosylation is an important post-translational modification involved in the modulation of a wide variety of cellular processes. Because glycosydases are central, the aim of this study was to investigate the glycosyl activity present in the cotyledons of the seeds of an important crop legume, Lupinus albus, as well a...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.03.009
更新日期：2013-08-15 00:00:00
abstract：:Salicylic acid (SA) is known to affect photosynthesis under normal conditions and induces tolerance in plants to biotic and abiotic stresses through influencing physiological processes. In this study, physiological processes were compared in salt-tolerant (Pusa Vishal) and salt-sensitive (T44) cultivars of mungbean an...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.11.001
更新日期：2011-05-15 00:00:00
abstract：:Cell suspension cultures of Silybum marianum are able to excrete silymarin compounds into the medium upon elicitation with methyl jasmonate or cyclodextrins. Knowledge of transport mechanism is important to understand Sm metabolism and to develop strategies aimed at increasing production by means of cell cultures. For...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2013.10.005
更新日期：2014-01-15 00:00:00
abstract：:We examined the functioning of the antioxidative defense system in Arabidopsis thaliana under sulphur (S) deficiency with an emphasis on the role of mitochondria. In tissue extracts and in isolated mitochondria from S-deficient plants, the concentration of non-protein thiols declined but protein thiols did not change....
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2015.07.011
更新日期：2015-08-15 00:00:00
abstract：:In open-field soilless culture there can be great deal of leaching, particularly in rainy springs. Ligneous plants have the capacity to store large quantities of nutrients in perennial organs. Knowledge of the plant's internal nutrient mobilisation during spring to supply growing organs could lead to reduction of fert...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2006.03.012
更新日期：2007-05-01 00:00:00
abstract：:The anthranilate synthase ASA1, CYP79B2 and CYP71B15 (PAD3) are biosynthetic genes of the Arabidopsis phytoalexin camalexin, which are induced after pathogen infection and abiotic treatments like silver nitrate spraying. The natural variation of camalexin biosynthesis in response to Pseudomonas syringae infection was ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2006.04.012
更新日期：2007-05-01 00:00:00
abstract：:Cryptochromes are blue-light photoreceptors that control many aspects of plant development. In this study, cryptochrome mutants of Arabidopsis were examined to assess the role of cryptchrome-1 (CRY1) in lateral roots growth. When grown in blue light for 12d, mutant seedlings (cry1) showed increased growth of lateral r...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.12.003
更新日期：2010-05-15 00:00:00
abstract：:The leaf is considered the most important vegetative organ of tank epiphytic bromeliads due to its ability to absorb and assimilate nutrients. However, little is known about the physiological characteristics of nutrient uptake and assimilation. In order to better understand the mechanisms utilized by some tank epiphyt...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2011.01.008
更新日期：2011-07-15 00:00:00
abstract：:Increasing atmospheric CO₂ concentrations are expected to enhance plant photosynthesis and yield. Nevertheless, after long-term exposure, plants acclimate and show a reduction in photosynthetic activity (called down-regulation), which may cause a reduction in potential yield. Some authors suggest that down-regulation ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2010.06.015
更新日期：2010-12-15 00:00:00
abstract：:In the highly vacuolated epidermis cells of leaf explants of Nautilocalyx we investigated whether inhibition of cytokinesis by 2,6-dichlorobenzonitrile (DCB) could be caused by a suppression of the formation of phragmosome and band of microtubules (BMT), two structures which are probably involved in cytokinesis and in...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/S0176-1617(84)80092-9
更新日期：1984-09-01 00:00:00
abstract：:Paeonia ostii var. lishizhenii, a well-known medicinal and horticultural plant, is indigenous to China. Recent studies have shown that its seed has a high oil content, and it was approved as a novel resource of edible oil with a high level of α-linolenic acid by the Chinese Government. This study measured the seed oil...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2018.05.011
更新日期：2018-09-01 00:00:00
abstract：:The assimilation and availability of nitrogen in its different forms can significantly affect the response of primary productivity under the current atmospheric alteration and soil degradation. An elevated CO2 concentration (e[CO2]) triggers changes in the efficiency and efficacy of photosynthetic processes, water use...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2016.05.020
更新日期：2016-08-01 00:00:00
abstract：:Plants resistant to triazine-type herbicides are known to be altered in their photosystem II reaction center. Serine at site 264 in D1 protein is replaced by glycine. The measurements of chlorophyll a fluorescence excitations with a variable number of saturating flashes in Chenopodium album plants show characteristic ...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2009.04.011
更新日期：2009-10-15 00:00:00
abstract：:The effects of three N(6)-substituted aminopurine derivatives containing either allenic or acetylenic side-chains on in vitro and in vivo cytokinin dehydrogenase (CKX; EC 1.5.99.12) activities were determined. At concentrations < or = 100 microM, the acetylenic derivative (HA-2) had no effect on in vitro CKX activity....
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.03.012
更新日期：2005-11-01 00:00:00
abstract：:Plants are able to acclimate to highly fluctuating light environment and evolved a short- and long-term light acclimatory responses, that are dependent on chloroplasts retrograde signalling. In this review we summarise recent evidences suggesting that the chloroplasts act as key sensors of light intensity changes in a...
journal_title：Journal of plant physiology
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.jplph.2013.06.005
更新日期：2013-11-15 00:00:00
abstract：:The effects of high salinity (0-400 mmol/L NaCl) on photosystem II (PSII) photochemistry and photosynthetic pigment composition were investigated in the halophyte Artimisia anethifolia grown under outdoor conditions and exposed to full sunlight. High salinity resulted in an inhibition in plant growth and a significant...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1078/0176-1617-00839
更新日期：2003-04-01 00:00:00
abstract：:The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-spe...
journal_title：Journal of plant physiology
pub_type： 杂志文章
doi：10.1016/j.jplph.2005.04.018
更新日期：2005-11-01 00:00:00
