当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY期刊下所有文献 > Application of real-time RT-PCR to study gene expression in active dry yeast (ADY) during the rehydration phase.

Application of real-time RT-PCR to study gene expression in active dry yeast (ADY) during the rehydration phase.

Abstract：

:During the industrial production of active dry yeast (ADY) and its subsequent use in winemaking, the yeast cell is subjected to drastic environmental changes that force it to undergo extensive metabolic modifications and changes in gene expression. In this study, we describe the use of real-time reverse transcription-polymerase chain reaction (RT-PCR) to monitor gene expression in ADY Saccharomyces cerevisiae during rehydration in different media. We used three statistical approaches to investigate the expression stability of eight potential reference genes during the rehydration process. The reference system thus obtained was used to normalize the expression values of three genes codifying for the ammonium transporters -- MEP1, MEP2, and MEP3 -- and two genes involved in the osmotic response-SIP18 and GPD1. The results suggested that for the target genes tested, the yeast reacted immediately to rehydration only when a fermentable carbon source was present in the medium. Furthermore, MEP2 expression was modulated by the ammonium concentration, indicating that nitrogen catabolite repression (NCR) is active during the rehydration phase.

journal_name

Int J Food Microbiol

journal_title

International journal of food microbiology

authors

Vaudano E,Costantini A,Cersosimo M,Del Prete V,Garcia-Moruno E

doi

10.1016/j.ijfoodmicro.2008.10.027

subject

Has Abstract

pub_date

2009-01-31 00:00:00

pages

30-6

issue

1

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(08)00569-2

journal_volume

129

pub_type

杂志文章

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