Abstract:
:Time to detection (TTD) measurements using turbidometry allow a straightforward method for the measurement of bacterial growth rates under isothermal conditions. Growth rate measurements were carried out for Listeria monocytogenes at 25, 30 and 37°C and for Pseudomonas aeruginosa over the temperature range 25 to 45°C. The classical three-parameter logistic model was rearranged to provide the theoretical foundation for the observed TTD. A model was subsequently developed for the analysis of TTD data from non-isothermal studies based on the Malthusian approximation of the logistic model. The model was able to predict the TTD for cultures of L. monocytogenes or P. aeruginosa undergoing simple temperature shunts (e.g. 25 to 37°C and vice versa), and for a multiple temperature shunt for L. monocytogenes (25-37-25-37°C and 37-25-37-25°C) over a period of 24h. In no case did a temperature shunt induce a lag.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Salih M,Mytilinaios I,Schofield HK,Lambert RJdoi
10.1016/j.ijfoodmicro.2012.01.011subject
Has Abstractpub_date
2012-04-02 00:00:00pages
29-35issue
1-2eissn
0168-1605issn
1879-3460pii
S0168-1605(12)00040-2journal_volume
155pub_type
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