Culture media for the isolation and enumeration of pathogenic Vibrio species in foods and environmental samples.

Abstract:

:The genus Vibrio now includes a large number of species. Clear evidence is only available for the aetiological role of V. cholerae, V. vulnificus and V. parahaemolyticus in foodborne diseases. Until recently, V. cholerae serogroup 0:1 was accepted as the cause of epidemic cholera. However, the designation of outbreaks of diarrhoeal diseases caused by V. cholerae 0:139 as clinical cholera has lead to renewed interest in Non 0:1 serogroups of V. cholerae. A wide range of enrichment and selective media for the isolation of vibrios has been developed. These media are reviewed with respect to their ability to recover and differentiate the target vibrios. Alkaline peptone water (APW) remains the recommended enrichment medium for vibrios in parallel with either salt polymyxin broth (SPB) or glucose teepol (or sodium dodecylsulphate) salt broth (GTSB) when tests for V. parahaemolyticus are required. Thiosulphate citrate bile salt agar (TCBS) in parallel with polymyxin mannose tellurite (PMT) or sodium dodecylsulphate polymyxin sucrose agar (SPS) are the recommended selective plating media.

journal_name

Int J Food Microbiol

authors

Donovan TJ,van Netten P

doi

10.1016/0168-1605(95)00015-c

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

77-91

issue

1

eissn

0168-1605

issn

1879-3460

pii

0168-1605(95)00015-C

journal_volume

26

pub_type

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