当前位置： SCI文献检索 > JOURNAL OF PLANT PHYSIOLOGY期刊下所有文献 > Salicylic acid induces H2O2 production and endochitinase gene expression but not ethylene biosynthesis in Castanea sativa in vitro model system.

Salicylic acid induces H2O2 production and endochitinase gene expression but not ethylene biosynthesis in Castanea sativa in vitro model system.

Abstract：

:Salicylic acid (SA), ethylene (ET), and wounding are all known to influence plant defense response. Experiments attempting to determine SA's relation to ET biosynthesis and defense gene expression have shown conflicting results. To confront this, we developed an in vitro model system to investigate how SA affects ET biosynthesis, hydrogen peroxide (H(2)O(2)) production and endochitinase gene expression in the European chestnut. ET measurements of in vitro shoots indicated a critical time point for SA exogenous application, enabling us to study its effects independent of ET. In addition, ET measurements demonstrated that its own increased biosynthesis was a response to wounding but not to SA treatment. Application of the ET biosynthesis inhibitor, aminoethoxyvinylglycine (AVG), on wounded and SA-treated shoots blocked wounding-induced ET production. Interestingly, SA inhibited ET production, but to a lesser extent than AVG. Additionally, SA also induced the accumulation of endochitinase transcript level. Likewise, a sensitive tissue-print assay showed that SA further increased the level of H(2)O(2). Yet, SA-induced endochitinase gene expression and SA-enhanced H(2)O(2) production levels were independent of ET. The cumulative results indicate that SA acts as an inducer of endochitinase PR gene expression and of H(2)O(2) oxidative burst. This suggests that SA is a component of the signal transduction pathway leading to defense against pathogens in chestnut. Further, the model system developed for this experiment should facilitate the deciphering of defense signaling pathways and their cross-talk. Moreover, it should also benefit the study of trees of long generation time that are known to be recalcitrant to in vitro studies.

journal_name

J Plant Physiol

journal_title

Journal of plant physiology

authors

Harfouche AL,Rugini E,Mencarelli F,Botondi R,Muleo R

doi

10.1016/j.jplph.2007.03.010

subject

Has Abstract

pub_date

2008-05-05 00:00:00

pages

734-44

issue

eissn

0176-1617

issn

1618-1328

pii

S0176-1617(07)00150-2

journal_volume

165

pub_type

杂志文章

在线工具

Salicylic acid induces H2O2 production and endochitinase gene expression but not ethylene biosynthesis in Castanea sativa in vitro model system.