Abstract：
:Microbial population dynamics were studied during the start-up and stabilization periods in thermophilic-dry anaerobic digestion at lab-scale. The experimental protocol was defined to quantify Eubacteria and Archaea using Fluorescent in situ hybridization (FISH) in a continuously stirred tank reactor (CSTR), without recycling solids. The reactor was subjected to a programme of steady-state operation over a range of the retention times from 40 to 25 days, with an organic loading rate between 4.42 and 7.50 kg volatile solid/m3/day. Changes in microbial concentrations were linked to traditional performance parameters such as biogas production and VS removal. The relations of Eubacteria:Archaea and H2-utilising methanogens:acetate-utilising methanogens were 88:12 and 11:1, respectively, during start-up stage. Hydrogenotrophic methanogens, although important in the initial phase of the reactor start-up, were displaced by acetoclastic methanogens at steady-state, thus their relation were 7:32, respectively. The methane yield coefficient, the methane content in the biogas and VS removal were stabilized around 0.30 LCH4/gCOD, 50% and 80%, respectively. Methanogenic population correlated well with performance measurements.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Montero B,Garcia-Morales JL,Sales D,Solera Rdoi
10.1016/j.biortech.2007.05.063subject
Has Abstractpub_date
2008-05-01 00:00:00pages
3233-43issue
8eissn
0960-8524issn
1873-2976pii
S0960-8524(07)00488-9journal_volume
99pub_type
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更新日期：2011-05-01 00:00:00
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更新日期：2001-05-01 00:00:00
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