Development and application of Multiple-Locus Variable number of tandem repeat Analysis (MLVA) to subtype a collection of Listeria monocytogenes.

Abstract:

:In this study we report the development and application of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) strategy for subtyping Listeria monocytogenes. Genome profiles of a collection of forty-five food-borne L. monocytogenes isolates were compared using MLVA. These isolates were obtained as part of an active surveillance programme of foods in the south-east region of Ireland. MLVA successfully discriminated amongst the isolates. The method was easy to perform, relatively fast and could be deployed in any molecular laboratory with basic laboratory equipment. This approach is a valuable tool, which has the capability to provide comparable results when compared with other more established typing methods, including pulsed-field gel electrophoresis (PFGE).

journal_name

Int J Food Microbiol

authors

Murphy M,Corcoran D,Buckley JF,O'Mahony M,Whyte P,Fanning S

doi

10.1016/j.ijfoodmicro.2006.10.022

subject

Has Abstract

pub_date

2007-04-10 00:00:00

pages

187-94

issue

2

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(06)00565-4

journal_volume

115

pub_type

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