Abstract:
:In this study we report the development and application of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) strategy for subtyping Listeria monocytogenes. Genome profiles of a collection of forty-five food-borne L. monocytogenes isolates were compared using MLVA. These isolates were obtained as part of an active surveillance programme of foods in the south-east region of Ireland. MLVA successfully discriminated amongst the isolates. The method was easy to perform, relatively fast and could be deployed in any molecular laboratory with basic laboratory equipment. This approach is a valuable tool, which has the capability to provide comparable results when compared with other more established typing methods, including pulsed-field gel electrophoresis (PFGE).
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Murphy M,Corcoran D,Buckley JF,O'Mahony M,Whyte P,Fanning Sdoi
10.1016/j.ijfoodmicro.2006.10.022subject
Has Abstractpub_date
2007-04-10 00:00:00pages
187-94issue
2eissn
0168-1605issn
1879-3460pii
S0168-1605(06)00565-4journal_volume
115pub_type
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