Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936.


:Polymerase chain reaction (PCR) assessment of clonal T-cell receptor (TCR) and immunoglobulin (Ig) gene rearrangements is an important diagnostic tool in mature T-cell neoplasms. However, lack of standardized primers and PCR protocols has hampered comparability of data in previous clonality studies. To obtain reference values for Ig/TCR rearrangement patterns, 19 European laboratories investigated 188 T-cell malignancies belonging to five World Health Organization-defined entities. The TCR/Ig spectrum of each sample was analyzed in duplicate in two different laboratories using the standardized BIOMED-2 PCR multiplex tubes accompanied by international pathology panel review. TCR clonality was detected in 99% (143/145) of all definite cases of T-cell prolymphocytic leukemia, T-cell large granular lymphocytic leukemia, peripheral T-cell lymphoma (unspecified) and angioimmunoblastic T-cell lymphoma (AILT), whereas nine of 43 anaplastic large cell lymphomas did not show clonal TCR rearrangements. Combined use of TCRB and TCRG genes revealed two or more clonal signals in 95% of all TCR clonal cases. Ig clonality was mostly restricted to AILT. Our study indicates that the BIOMED-2 multiplex PCR tubes provide a powerful strategy for clonality assessment in T-cell malignancies assisting the firm diagnosis of T-cell neoplasms. The detected TCR gene rearrangements can also be used as PCR targets for monitoring of minimal residual disease.






Brüggemann M,White H,Gaulard P,Garcia-Sanz R,Gameiro P,Oeschger S,Jasani B,Ott M,Delsol G,Orfao A,Tiemann M,Herbst H,Langerak AW,Spaargaren M,Moreau E,Groenen PJ,Sambade C,Foroni L,Carter GI,Hummel M,Bastard C,D




Has Abstract


2007-02-01 00:00:00
















  • Schedule-dependent efficacy of idarubicin (Ida) and doxorubicin (Dox).

    abstract::Schedule dependency of idarubicin (Ida) and doxorubicin (Dox) toxicity was investigated in vitro using the K562 human leukemia cell line. For Dox, repeated exposure to the IC30 (d x 3) resulted in comparable survival as single exposure to the total accumulative dose (20%). For Ida, repeated exposure to the IC30 (d x 3...


    pub_type: 杂志文章


    authors: Minderman H,Buscaglia MD,Rustum YM

    更新日期:1994-08-01 00:00:00

  • Methylation analysis of the human multidrug resistance 1 gene in normal and leukemic hematopoietic cells.

    abstract::Expression of the human multidrug resistance 1 gene (MDR1), which encodes the P-glycoprotein transmembrane efflux pump, has been associated with treatment failure of some leukemias, primarily acute myeloid leukemia (AML). To elucidate the epigenetic events associated with overexpression of MDR1 in AML, we analyzed the...


    pub_type: 杂志文章


    authors: Fryxell KB,McGee SB,Simoneaux DK,Willman CL,Cornwell MM

    更新日期:1999-06-01 00:00:00

  • Rates of peripheral arterial occlusive disease in patients with chronic myeloid leukemia in the chronic phase treated with imatinib, nilotinib, or non-tyrosine kinase therapy: a retrospective cohort analysis.

    abstract::Peripheral arterial occlusive disease (PAOD) occurs in patients with chronic phase chronic myeloid leukemia (CML-CP) treated with tyrosine kinase inhibitors (TKIs). The risk of developing PAOD on TKI therapy is unknown and causality has not been established. Patients with CML-CP from three randomized phase III studies...


    pub_type: 杂志文章


    authors: Giles FJ,Mauro MJ,Hong F,Ortmann CE,McNeill C,Woodman RC,Hochhaus A,le Coutre PD,Saglio G

    更新日期:2013-06-01 00:00:00

  • Childhood monosomy 7 syndrome: clinical and in vitro studies.

    abstract::The clinical and cell growth characteristics of 11 children with monosomy 7 presenting as preleukemia (eight cases) or acute nonlymphoblastic leukemia (three cases) were studied. Anemia was common to all patients, with nine showing leukocytosis, seven thrombocytopenia, and one thrombocytosis. There was a striking pred...


    pub_type: 杂志文章


    authors: Weiss K,Stass S,Williams D,Kalwinsky D,Dahl GV,Wang W,Johnson FL,Murphy SB,Dow LW

    更新日期:1987-02-01 00:00:00

  • Different isoforms of the B-cell mutator activation-induced cytidine deaminase are aberrantly expressed in BCR-ABL1-positive acute lymphoblastic leukemia patients.

    abstract::The main reason for the unfavorable clinical outcome of BCR-ABL1-positive acute lymphoblastic leukemia (ALL) is genetic instability. However, how normal B-cell precursors acquire the genetic changes that lead to transformation has not yet been completely defined. We investigated the expression of the activation-induce...


    pub_type: 杂志文章


    authors: Iacobucci I,Lonetti A,Messa F,Ferrari A,Cilloni D,Soverini S,Paoloni F,Arruga F,Ottaviani E,Chiaretti S,Messina M,Vignetti M,Papayannidis C,Vitale A,Pane F,Piccaluga PP,Paolini S,Berton G,Baruzzi A,Saglio G,Baccar

    更新日期:2010-01-01 00:00:00

  • Principal component analysis uncovers cytomegalovirus-associated NK cell activation in Ph+ leukemia patients treated with dasatinib.

    abstract::Dasatinib treatment markedly increases the number of large granular lymphocytes (LGLs) in a proportion of Ph+ leukemia patients, which associates with a better prognosis. The lymphocytosis is predominantly observed in cytomegalovirus (CMV)-seropositive patients, yet detectable CMV reactivation exists only in a small f...


    pub_type: 杂志文章


    authors: Ishiyama K,Kitawaki T,Sugimoto N,Sozu T,Anzai N,Okada M,Nohgawa M,Hatanaka K,Arima N,Ishikawa T,Tabata S,Onaka T,Oka S,Nakabo Y,Amakawa R,Matsui M,Moriguchi T,Takaori-Kondo A,Kadowaki N

    更新日期:2017-01-01 00:00:00

  • Derivative chromosome 9 deletions are a significant feature of childhood Philadelphia chromosome positive acute lymphoblastic leukaemia.

    abstract::Deletions from the derivative chromosome 9, der(9), of the translocation, t(9;22)(q34;q11), at the site of the ABL/BCR fusion gene, have been demonstrated by fluorescence in situ hybridisation (FISH), in both Philadelphia chromosome (Ph)-positive chronic myeloid leukaemia (CML) and acute lymphoblastic leukaemia (ALL)....


    pub_type: 杂志文章


    authors: Robinson HM,Martineau M,Harris RL,Barber KE,Jalali GR,Moorman AV,Strefford JC,Broadfield ZJ,Cheung KL,Harrison CJ

    更新日期:2005-04-01 00:00:00

  • Telomere dynamics in childhood leukemia and solid tumors: a follow-up study.

    abstract::Telomeres of hematopoietic cells shorten with age, possibly contributing to the aging-associated hematopoietic pathology (immunosenescence, malignant transformation). Accelerated telomere shortening is seen with replicative stress, such as during administration of serial chemotherapy cycles for the treatment of childh...


    pub_type: 杂志文章


    authors: Franco S,Ozkaynak MF,Sandoval C,Tugal O,Jayabose S,Engelhardt M,Moore MA

    更新日期:2003-02-01 00:00:00

  • Double induction strategy including high dose cytarabine in combination with all-trans retinoic acid: effects in patients with newly diagnosed acute promyelocytic leukemia. German AML Cooperative Group.

    abstract::A prospective multicenter study was performed to investigate the clinical and molecular results of intensified double induction therapy including high-dose cytarabine (ara-C) in combination with ATRA in newly diagnosed acute promyelocytic leukemia (APL), followed by consolidation and 3 years maintenance therapy. Fifty...


    pub_type: 临床试验,杂志文章,多中心研究


    authors: Lengfelder E,Reichert A,Schoch C,Haase D,Haferlach T,Löffler H,Staib P,Heyll A,Seifarth W,Saussele S,Fonatsch C,Gassmann W,Ludwig WD,Hochhaus A,Beelen D,Aul C,Sauerland MC,Heinecke A,Hehlmann R,Wörmann B,Hiddemann

    更新日期:2000-08-01 00:00:00

  • The effects of interleukin-3, GM-CSF, and G-CSF on the growth kinetics of colony-forming cells in myelodysplastic syndromes.

    abstract::In order to obtain more insight into the nature of the abnormal in vitro colony formation in myelodysplastic syndromes (MDS), we investigated the kinetics of the colony formation of 23 MDS cases in response to recombinant human interleukin-3 (IL-3), Granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyt...


    pub_type: 杂志文章


    authors: Schipperus M,Sonneveld P,Lindemans J,Hagemeijer A,Vink N,Pegels J,Abels J

    更新日期:1990-04-01 00:00:00

  • Internal tandem duplication of the flt3 gene found in acute myeloid leukemia.

    abstract::We analyzed mRNA expression of the flt3 gene in 30 patients with acute myeloid leukemia (AML) and 50 with acute lymphoblastic leukemia (ALL). Using reverse transcriptase-polymerase chain reaction (RT-PCR), expression of flt3 was observed in 61 patients; 22 (73%) with AML and 39 (78%) with ALL. Among these, five patien...


    pub_type: 杂志文章


    authors: Nakao M,Yokota S,Iwai T,Kaneko H,Horiike S,Kashima K,Sonoda Y,Fujimoto T,Misawa S

    更新日期:1996-12-01 00:00:00

  • Epstein-Barr virus in T and natural killer (NK) cell non-Hodgkin's lymphomas.

    abstract::Several studies using sensitive in situ hybridization techniques show that, in non-immunocompromised patients, Epstein-Barr virus (EBV) is more frequently detected in lymphomas expressing T cell markers than in B cell lymphomas. Among lymphomas expressing T cell markers, the presence of EBV is highly related to the si...


    pub_type: 杂志文章,评审


    authors: Kanavaros P,Briere J,Emile JF,Gaulard P

    更新日期:1996-06-01 00:00:00

  • Synthetic triterpenoids activate a pathway for apoptosis in AML cells involving downregulation of FLIP and sensitization to TRAIL.

    abstract::Acute myelogenous leukemia (AML) remains a deadly disease for most adult patients, due primarily to the emergence of chemoresistant cells. Defects in apoptosis pathways make important contributions to chemoresistance, suggesting a need to restore apoptosis sensitivity or to identify alternative pathways for apoptosis ...


    pub_type: 杂志文章


    authors: Suh WS,Kim YS,Schimmer AD,Kitada S,Minden M,Andreeff M,Suh N,Sporn M,Reed JC

    更新日期:2003-11-01 00:00:00

  • Monoclonal antibodies specific for P-glycoprotein.

    abstract::Multidrug resistance (MDR) is one of the major obstacles to successful cancer chemotherapy. Since P-glycoprotein (P-gp) encoded by the MDR-1 gene plays a key role in MDR, many P-gp-specific monoclonal antibodies (MAbs) have been generated for characterization and analysis of P-gp. Among those antibodies, MRK16 has bee...


    pub_type: 杂志文章,评审


    authors: Okochi E,Iwahashi T,Tsuruo T

    更新日期:1997-07-01 00:00:00

  • Insulin is a potent myeloma cell growth factor through insulin/IGF-1 hybrid receptor activation.

    abstract::Insulin and insulin growth factor type 1 (IGF-1) and their receptors are closely related molecules, but both factors bind to the receptor of the other one with a weak affinity. No study has presently documented a role of insulin as a myeloma growth factor (MGF) for human multiple myeloma cells (MMCs), whereas many stu...


    pub_type: 杂志文章


    authors: Sprynski AC,Hose D,Kassambara A,Vincent L,Jourdan M,Rossi JF,Goldschmidt H,Klein B

    更新日期:2010-11-01 00:00:00

  • Polyclonal expansion of T-cell receptor-gamma delta+ T lymphocytes associated with neutropenia and thrombocytopenia.

    abstract::A patient with large granular lymphocyte (LGL) expansion (T-gamma lymphocytosis), neutropenia and thrombocytopenia was studied longitudinally. Analysis of peripheral blood mononuclear cells (PBMC) demonstrated an unusual large proportion of CD3+ T-lymphocytes expressing a gamma delta T-cell receptor (TcR-gamma delta)....


    pub_type: 杂志文章


    authors: van Oostveen JW,Breit TM,de Wolf JT,Brandt RM,Smit JW,van Dongen JJ,Borst J,Melief CJ

    更新日期:1992-05-01 00:00:00

  • Flow cytometric analysis of intercellular adhesion between B-cell precursor acute lymphoblastic leukemic cells and bone marrow stromal cells.

    abstract::The growth of B-cell precursor acute lymphoblastic leukemic (BCP ALL) cells in vitro is dependent on interactions with bone marrow (BM) stromal cells. We have recently demonstrated that the rate of cell division of BCP ALL cells increases when cultured in direct contact with BM stromal cells. In this study we describe...


    pub_type: 杂志文章


    authors: Ashley DM,Bol SJ,Tucker DP,Waugh CM,Kannourakis G

    更新日期:1995-01-01 00:00:00

  • Impact of pretransplant minimal residual disease after cord blood transplantation for childhood acute lymphoblastic leukemia in remission: an Eurocord, PDWP-EBMT analysis.

    abstract::To address the prognostic value of minimal residual disease (MRD) before unrelated cord blood transplantation (UCBT) in children with acute lymphoblastic leukemia (ALL), we analyzed 170 ALL children transplanted in complete remission (CR) after myeloablative conditioning regimen. In all, 72 (43%) were in first CR (CR1...


    pub_type: 杂志文章,多中心研究


    authors: Ruggeri A,Michel G,Dalle JH,Caniglia M,Locatelli F,Campos A,de Heredia CD,Mohty M,Hurtado JM,Bierings M,Bittencourt H,Mauad M,Purtill D,Cunha R,Kabbara N,Gluckman E,Labopin M,Peters C,Rocha V

    更新日期:2012-12-01 00:00:00

  • Homing and mobilization of hematopoietic stem cells and hematopoietic cancer cells are mirror image processes, utilizing similar signaling pathways and occurring concurrently: circulating cancer cells constitute an ideal target for concurrent treatment wi

    abstract::Adhesion molecules and stromal cell-derived factor-1 (SDF-1)/CXCR4 signaling play key role in homing and mobilization of hematopoietic progenitor (HPC) and hematopoietic cancer clonogenic cells (HCC). High expression of VLA-4 is required for homing of HPC and HCC, whereas downregulation of these molecules is required ...


    pub_type: 杂志文章,评审


    authors: Gazitt Y

    更新日期:2004-01-01 00:00:00

  • γδT cells elicited by CMV reactivation after allo-SCT cross-recognize CMV and leukemia.

    abstract::Human cytomegalovirus (CMV) infections and relapse of disease remain major problems after allogeneic stem cell transplantation (allo-SCT), in particular in combination with CMV-negative donors or cordblood transplantations. Recent data suggest a paradoxical association between CMV reactivation after allo-SCT and reduc...


    pub_type: 杂志文章


    authors: Scheper W,van Dorp S,Kersting S,Pietersma F,Lindemans C,Hol S,Heijhuurs S,Sebestyen Z,Gründer C,Marcu-Malina V,Marchant A,Donner C,Plachter B,Vermijlen D,van Baarle D,Kuball J

    更新日期:2013-06-01 00:00:00

  • Outcome in children with Down's syndrome and acute lymphoblastic leukemia: role of IKZF1 deletions and CRLF2 aberrations.

    abstract::Children with Down's syndrome (DS) have an increased risk of developing acute lymphoblastic leukemia (ALL) and have a low frequency of established genetic aberrations. We aimed to determine which genetic abnormalities are involved in DS ALL. We studied the frequency and prognostic value of deletions in B-cell developm...


    pub_type: 杂志文章


    authors: Buitenkamp TD,Pieters R,Gallimore NE,van der Veer A,Meijerink JP,Beverloo HB,Zimmermann M,de Haas V,Richards SM,Vora AJ,Mitchell CD,Russell LJ,Schwab C,Harrison CJ,Moorman AV,van den Heuvel-Eibrink MM,den Boer ML,Zwaan

    更新日期:2012-10-01 00:00:00

  • Alpha interferon gene deletions in adults, children and infants with acute lymphoblastic leukemia.

    abstract::DNA from 76 cases of acute lymphoblastic leukemia (ALL) was tested with a cDNA probe encoding the alpha 2B interferon (IFN) gene transcript. Deletions were found in three of ten pre-B, three of 21 T-cell, four of 22 common and one of 23 null ALL cases. Amongst those with null ALL were 20 infants, most with characteris...


    pub_type: 杂志文章


    authors: Middleton PG,Prince RA,Williamson IK,Taylor PR,Reid MM,Jackson GH,Katz F,Chessells JM,Proctor SJ

    更新日期:1991-08-01 00:00:00

  • Inhibition of CXCR4 in CML cells disrupts their interaction with the bone marrow microenvironment and sensitizes them to nilotinib.

    abstract::Drug resistance is a growing area of concern. It has been shown that a small, residual pool of leukemic CD34+ progenitor cells can survive in the marrow microenvironment of chronic myeloid leukemia (CML) patients after years of kinase inhibitor treatment. Bone marrow (BM) stroma has been implicated in the long-term su...


    pub_type: 杂志文章


    authors: Weisberg E,Azab AK,Manley PW,Kung AL,Christie AL,Bronson R,Ghobrial IM,Griffin JD

    更新日期:2012-05-01 00:00:00

  • A novel CD10-positive erythroid cell line, RM10, established from a patient with chronic myelogenous leukemia.

    abstract::A novel erythroid cell line, RM10, was established from a long-term bone marrow culture of a patient with chronic myelogenous leukemia (CML). RM10 cells were positive for periodic acid Schiff (PAS), but negative for peroxidase and dual esterase. RM10 cells had la, pre B (CD10), myeloid (CD13, CD14, CD33) and erythroid...


    pub_type: 杂志文章


    authors: Hirata J,Sato H,Takahira H,Shiokawa S,Endo T,Nishimura J,Katsuno M,Masuda S,Sasaki R,Fukumaki Y

    更新日期:1990-05-01 00:00:00

  • Long-term follow-up of reduced-intensity allogeneic stem cell transplantation for chronic lymphocytic leukemia: prognostic model to predict outcome.

    abstract::Chronic lymphocytic leukemia (CLL) remains incurable with chemoimmunotherapy, and allogeneic hematopoietic stem cell transplantation (HSCT) offers the potential for cure. We assessed the outcomes of 108 CLL patients undergoing first allogeneic HSCTs, 76 with reduced-intensity (RIC) and 32 with myeloablative conditioni...


    pub_type: 杂志文章


    authors: Brown JR,Kim HT,Armand P,Cutler C,Fisher DC,Ho V,Koreth J,Ritz J,Wu C,Antin JH,Soiffer RJ,Gribben JG,Alyea EP

    更新日期:2013-02-01 00:00:00

  • Treatment strategies and long-term results in paediatric patients treated in four consecutive AML-BFM trials.

    abstract::A total of 1111 children with acute myeloblastic leukaemia (AML) were treated in four consecutive Berlin-Frankfurt-Münster (BFM) studies from 1978 to 1998. The first cooperative trial AML-BFM 78 established intensive chemotherapy with seven drugs, CNS irradiation and 2-year maintenance, achieving a long-term survival ...


    pub_type: 临床试验,杂志文章


    authors: Creutzig U,Zimmermann M,Ritter J,Reinhardt D,Hermann J,Henze G,Jürgens H,Kabisch H,Reiter A,Riehm H,Gadner H,Schellong G

    更新日期:2005-12-01 00:00:00

  • The benzene metabolite, hydroquinone, induces dose-dependent hypoploidy in a human cell line.

    abstract::Chronic exposure to high concentrations of benzene can result in the development of myelodysplastic syndrome (MDS) and acute myelogenous leukemia (AML). Studies of patients occupationally exposed to benzene show a pattern of cytogenetic aberrations involving high frequency of loss of all or part of chromosomes 5 and/o...


    pub_type: 杂志文章


    authors: Stillman WS,Varella-Garcia M,Gruntmeir JJ,Irons RD

    更新日期:1997-09-01 00:00:00

  • Spontaneous responsiveness to cytokines by human T-cell leukemias.

    abstract::The molecular basis for autonomous growth of malignant forms of human T lymphocytes is not known. It can be investigated by functional responses of malignant cells in comparison with untransformed counterparts. At least two pathways (the CD2 and CD3 pathways) of human T-cell activation have been recently defined on th...


    pub_type: 杂志文章


    authors: Schirren CA,Völpel H,Meuer SC

    更新日期:1992-06-01 00:00:00

  • Long-term outcome of patients with newly diagnosed chronic myeloid leukemia: a randomized comparison of stem cell transplantation with drug treatment.

    abstract::Tyrosine kinase inhibitors represent today's treatment of choice in chronic myeloid leukemia (CML). Allogeneic hematopoietic stem cell transplantation (HSCT) is regarded as salvage therapy. This prospective randomized CML-study IIIA recruited 669 patients with newly diagnosed CML between July 1997 and January 2004 fro...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Gratwohl A,Pfirrmann M,Zander A,Kröger N,Beelen D,Novotny J,Nerl C,Scheid C,Spiekermann K,Mayer J,Sayer HG,Falge C,Bunjes D,Döhner H,Ganser A,Schmidt-Wolf I,Schwerdtfeger R,Baurmann H,Kuse R,Schmitz N,Wehmeier A,

    更新日期:2016-03-01 00:00:00

  • Prognostic impact of RAS-pathway mutations in patients with myelofibrosis.

    abstract::RAS-pathway mutations are recurrent events in myeloid malignancies. However, there is limited data on the significance of RAS-pathway mutations in patients with myelofibrosis (MF). We analyzed next-generation sequencing data of 16 genes, including RAS-pathway genes, from 723 patients with primary and secondary MF acro...


    pub_type: 杂志文章,多中心研究


    authors: Santos FPS,Getta B,Masarova L,Famulare C,Schulman J,Datoguia TS,Puga RD,Alves Paiva RM,Arcila ME,Hamerschlak N,Kantarjian HM,Levine RL,Campregher PV,Rampal RK,Verstovsek S

    更新日期:2020-03-01 00:00:00