Abstract:
:Cell lines derived from cervical neoplasias, as well as cells from normal cervix and neonatal foreskin were examined in an in vitro culture system (raft system) that allows for stratification and differentiation of epithelial cells at an air-liquid interface. Epithelial cells from human foreskin and ectocervix were observed to differentiate in a manner histologically similar to normal epithelium in vivo as indicated by a single layer of basal cells with a defined mid and upper zone. In contrast, cells expanded from cervical squamous carcinoma explants showed total absence of normal differentiation in the raft system with numerous cells in the upper portion of the stratum exhibiting mitotic figures. Cell cultures derived from low grade cervical intraepithelial neoplasia and condyloma acuminata exhibited partial differentiation in addition to perinuclear clearing, binucleate cells and individual cell keratinization. These studies show that in the raft system, cultured cells derived from tissue biopsies can duplicate many of the histological features observed in cervical neoplasias. In addition, epithelial cells derived from normal fetal ectocervix and electroporated with cloned human papillomavirus (HPV) type 16 DNA exhibited abnormal differentiation patterns similar to those of cervical intraepithelial neoplasia in vivo. This model system will thus be useful for examining the effects of HPV infection on epithelial maturation and for investigating the role of other factors in the progression of cervical malignancies.
journal_name
Oncogenejournal_title
Oncogeneauthors
Rader JS,Golub TR,Hudson JB,Patel D,Bedell MA,Laimins LAsubject
Has Abstractpub_date
1990-04-01 00:00:00pages
571-6issue
4eissn
0950-9232issn
1476-5594journal_volume
5pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Heat shock protein 70 (HSP70) can inhibit apoptosis by neutralizing and interacting with apoptosis-inducing factor (AIF), a mitochondrial flavoprotein that translocates upon apoptosis induction to the nucleus, via the cytosol. Here, we show that only members of the HSP70 family interact with AIF. Systematic deletion m...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206794
更新日期:2003-10-02 00:00:00
abstract::The A-myb gene belongs to the family of the c-myb proto-oncogene. We report here the cloning from a B lymphocyte cDNA library of the previously missing 3' half of the human A-myb cDNA, thus closing the previously still incomplete open reading frame. Analysis of the homologies between the different myb proteins reveals...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase aberrantly expressed in neuroblastoma, a devastating pediatric cancer of the sympathetic nervous system. Germline and somatically acquired ALK aberrations induce increased autophosphorylation, constitutive ALK activation and increased downstream signaling....
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.647
更新日期:2012-11-15 00:00:00
abstract::Reduced co-expression of the c-fos and c-jun protooncogenes has been correlated with the down regulation of H-2K class I major histocompatibility antigens in high-metastatic cell lines from the Lewis lung carcinoma, B16 melanoma and the K1735 melanoma. Transfection of c-jun and c-fos genes into the high metastatic clo...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::We have found that a malignant mesothelioma cell line, NCI-H28, had a chromosome 3p21.3 homozygous deletion containing the beta-catenin gene (CTNNB1), which suggested that the deletion of beta-catenin might have a growth advantage in the development of this tumor. To determine whether beta-catenin has a growth-inhibit...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206533
更新日期:2003-09-11 00:00:00
abstract::Activation of the mitogen-activated protein kinase (MAPK) cascade is a well documented mechanism for the G-protein-coupled receptors. Here, we have analysed the requirements for ERKs and p38 MAPK activation by thrombin in Jurkat T cells. We show that thrombin-mediated ERKs activation requires both PTK and PKC activiti...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204266
更新日期:2001-04-12 00:00:00
abstract::B-Myb, a highly conserved member of the Myb oncoprotein family, is a 110 kDa sequence-specific DNA binding protein expressed in virtually all proliferating cells. B-myb expression reaches its maximum at the G1/S phase boundary and during the S phase of the cell cycle. We have previously shown that B-Myb activity is ce...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203618
更新日期:2000-06-15 00:00:00
abstract::Long-lived species Homo sapiens have evolved robust protection mechanisms against cancer by repressing telomerase and maintaining short telomeres, thereby delaying the onset of the majority of cancer types until post-reproductive age. Indeed, telomerase is silent in most differentiated human cells, predominantly due t...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/s41388-019-0872-9
更新日期:2019-08-01 00:00:00
abstract::The E2F1 transcription factor, which is deregulated in most human cancers by mutations in the p16-cyclin D-Rb pathway, has both oncogenic and tumor-suppressive properties. This is dramatically illustrated by the phenotype of an E2F1 transgenic mouse model that spontaneously develops tumors in the skin and other epithe...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209120
更新日期:2006-02-09 00:00:00
abstract::Integrins are adhesion receptors that mediate both cell-extracellular matrix and cell-cell interactions. It has also been reported that the loss of integrin alpha4 expression might be associated with metastasis in several cancers. However, the molecular mechanism for loss of their expression in cancers has not been ex...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207470
更新日期:2004-04-22 00:00:00
abstract::Dual specificity kinases that phosphorylate the Thr- and Tyr-residues within the TXY motif of MAP-kinases of play a central role in the regulation of various processes of cell growth. These dual specificity kinases also known as MAP kinase kinases are constituents of the sequential kinase signaling modules. Seven dist...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1202251
更新日期:1998-09-17 00:00:00
abstract::A potential role for 1-oleoyl-sn-glycero-3-phosphate or lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) in the regulation of malignant diseases has been widely considered. In this study, we found that in transformed astroglial cells, the expression profile of lysophospholipid receptor mRNA and the action...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208805
更新日期:2005-10-06 00:00:00
abstract::Latent membrane protein 1 (LMP1), an oncoprotein encoded by Epstein-Barr virus (EBV), is an integral membrane protein, which acts like a constitutively active receptor. LMP1 is critical for some facet of EBV's induction and maintenance of proliferation of infected B cells. It, in part, mimics signaling by the CD40 rec...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208367
更新日期:2005-03-03 00:00:00
abstract::The microenvironment of glioblastoma (GBM) contains high levels of inflammatory cytokine interleukin 6 (IL-6), which contributes to promote tumour progression and invasion. The common epidermal growth factor receptor variant III (EGFRvIII) mutation in GBM is associated with significantly higher levels of IL-6. Further...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.225
更新日期:2015-05-28 00:00:00
abstract::Myeloid differentiation is a highly regulated process governed by various cytokines, such as EPO, TPO, G-CSF, IL-3, IL-5 and GM-CSF. These cytokines act in part through activation of the STAT transcription factor family. In particular, various isoforms of STAT3 and STAT5 are activated during myeloid differentiation in...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1203479
更新日期:2000-05-15 00:00:00
abstract::The Ste20-like kinase, SLK, is involved in the control of cell motility through its effects on actin reorganization and focal adhesion turnover. Here we investigated the role of SLK in chemotaxis downstream of the tyrosine kinase receptor, HER2/ErbB2/Neu, which is frequently overexpressed in human breast cancers. Our ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.146
更新日期:2009-08-06 00:00:00
abstract::We have studied the role of the HPV-16 E5 protein in apoptosis, using HaCaT cell lines stably transfected with either E5 (HaCaT/E5) or the empty vector (HaCaT/pMSG) as control. When subjected to a hyperosmolar concentration of sorbitol, HaCaT/E5 cells respond with cytochrome c release, activation of caspase-3, -8, and...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205147
更新日期:2002-01-31 00:00:00
abstract::The role of the hepatitis B virus protein HBx in liver cell proliferation and apoptosis remains controversial. Using a transgenic mouse model, we have recently shown that HBx stimulates the apoptotic turnover of hepatocytes, independently of p53. In this paper, we tested whether the proapoptotic function of HBx can in...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205110
更新日期:2002-01-17 00:00:00
abstract::Exposure of CV-1P cells to hypoxic conditions results in reversible cell cycle arrest concomitant with accumulation of pRB in the hypophosphorylated, growth suppressive form. Similar to cell cycle arrest induced by serum starvation, we show here that hypoxia-induced arrest is accompanied by a decrease in pRB-directed ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202159
更新日期:1998-11-05 00:00:00
abstract::Cyclooxygenase-2 (COX-2) is an inducible enzyme that contributes to the generation of chronic inflammation in response to chemical carcinogens and environmental stresses, including ultraviolet B (UVB) irradiation. Although post-translational histone modifications are believed to have an important role in modulating tr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.71
更新日期:2013-01-24 00:00:00
abstract::Pretreatment of cells with 0.5 mM sodium arsenite (but not other activators of stress-activated MAP kinase cascades) prevents the activation of p21Ras and strongly suppresses the activation of c-Raf and the MAP kinase cascade by a variety of growth factors. Arsenite appears to exert its effect by preventing the guanin...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202168
更新日期:1998-07-09 00:00:00
abstract::Pancreatic cancer is characterized by early metastatic spread, but the process of tumor cell dissemination is largely unknown. In this study we show that the soluble protein pancreatic adenocarcinoma upregulated factor (PAUF) has an important role in the metastasis and progression of the disease. Variations in the lev...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.298
更新日期:2010-01-07 00:00:00
abstract::Histone acetylation has a central role in establishing an active chromatin environment. The functional contribution of histone acetylation to chromatin transcription is accomplished by a dominant action of histone acetyltransferases over repressive histone-modifying activities at gene promoters; misregulation of these...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.273
更新日期:2013-05-16 00:00:00
abstract::The cellular transcription factor Brn-3a differentially regulates different human papilloma virus (HPV)-16 variants that are associated with different risks of progression to cervical carcinoma in infected humans. The upstream regulatory regions (URRs) of high- and intermediate-risk HPV-16 variants are activated by th...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.33
更新日期:2010-05-06 00:00:00
abstract::The 120 kD product of the c-cbl oncogene is rapidly tyrosine phosphorylated and recruited to the EGF receptor following ligand binding. Cbl's oncogenic potential is activated by a large carboxy-terminal truncation that generated v-cbl and removes the Ring finger and proline-rich SH3-binding domains. Here we show that ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201193
更新日期:1997-05-08 00:00:00
abstract::The transcription factor Snail has been recently proposed as an important mediator of tumour invasion because of its role in downregulation of E-cadherin and induction of epithelial-mesenchymal transitions (EMT). This behaviour has led to the consideration of Snail as a potential therapeutic target to block tumour pro...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209997
更新日期:2007-03-22 00:00:00
abstract::Migration and invasion inhibitory protein (MIIP) is recently identified as an inhibitor in tumor development. However, the regulatory mechanism and biological contributions of MIIP in pancreatic cancer (PC) have been not elucidated. In this study, we demonstrated a negative feedback of MIIP and hypoxia-induced factor-...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-017-0082-2
更新日期:2018-03-01 00:00:00
abstract::The DCC (deleted in colorectal cancer) gene was originally identified as a candidate tumour suppressor gene in colon carcinogenesis on the basis of allelic losses in chromosome 18q.21 in 70% of colon cancers. Reverse transcriptase polymerase chain reaction (RT-PCR) of DCC mRNA suggests that DCC expression may also be ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-08-15 00:00:00
abstract::The t(12;21) translocation, generating the TEL/AML1 fusion protein, is the most common genetic lesion in childhood cancer. Using a bone marrow transplantation model, we demonstrate that TEL/AML1 expression impinges on normal hematopoietic differentiation, leading to the in vivo accumulation and persistence of an early...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208931
更新日期:2005-11-17 00:00:00
abstract::Proteolytic cleavage of the extracellular domain of CD44 from the surface of cells has been observed recently in different cell types. In cell culture supernatants of human melanoma cell lines a 70 kDa soluble CD44 protein (solCD44) was detected at concentrations of 250-300 ng/ml. Protease inhibitor studies revealed t...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204435
更新日期:2001-06-07 00:00:00