Abstract：
:To determine the prevalence of Clostridium botulinum types A, B, E, and/or F in pig intestinal samples, different PCR-based methods were compared using artificially contaminated faeces. The methods included a multiplex PCR targeted to botulinum neurotoxin genes A, B, E, and F or a nested PCR targeted to toxin genes B, E, and F, combined with different pre-enrichment protocols and forms of templates. A method including the two-step enrichment followed by DNA extraction and multiplex PCR yielded the highest number of positives. This assay protocol was employed to investigate 100 pig intestinal samples. The sample materials studied included colon wall, intestinal content, and mucus peeled from the colon wall. Three pigs (3%) were positive for C. botulinum type B, and no other toxinotypes were detected in any sample. The number of positive samples was higher when colon wall or peeled mucus was analyzed compared to the intestinal content. C. botulinum was isolated from two PCR-positive samples and confirmed to be type B by PCR. Both isolates were shown to be proteolytic and thus to represent C. botulinum group I.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Myllykoski J,Nevas M,Lindström M,Korkeala Hdoi
10.1016/j.ijfoodmicro.2006.04.017subject
Has Abstractpub_date
2006-07-15 00:00:00pages
172-7issue
2eissn
0168-1605issn
1879-3460pii
S0168-1605(06)00245-5journal_volume
110pub_type
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