Abstract:
:B-cell chronic lymphocytic leukaemia (B-CLL) is a slowly progressing malignancy of CD5(+) B cells, for which at present no curative treatment is available. In our current study, we apply a novel bridging reagent to redirect cytomegalovirus (CMV)-specific cytotoxic T lymphocytes (CTL) to target B-CLL. A streptavidin-fused anti-CD20 single-chain variable fragment (scFv) is used in combination with biotinylated MHC class I molecules containing CMV pp65 peptide (HLA/CMV). We demonstrate that B-CLL cells coated with this CD20-HLA/CMV complex can be lysed by autologous CMV-specific CTL with similar efficiency as B-CLL cells directly loaded with CMV peptide. Killing is HLA restricted and occurs at scFv CD20 concentrations of >/=100 ng ml(-1) and HLA/CMV concentrations of >/=20 ng ml(-1). Furthermore, complex-coated B-CLL cells induce both proliferation and cytokine production (interferon gamma, tumour necrosis factor alpha and macrophage inflammatory protein-1 beta) in CMV-specific CD8(+) T cells. Hereby, a necessary step towards possible application of CD20-HLA/CMV complexes for immunotherapy of B-cell malignancies is constituted.
journal_name
Leukemiajournal_title
Leukemiaauthors
Mous R,Savage P,Remmerswaal EB,van Lier RA,Eldering E,van Oers MHdoi
10.1038/sj.leu.2404185subject
Has Abstractpub_date
2006-06-01 00:00:00pages
1096-102issue
6eissn
0887-6924issn
1476-5551pii
2404185journal_volume
20pub_type
杂志文章相关文献
LEUKEMIA文献大全abstract::Continuous leukemia-lymphoma cell lines are important research tools, in particular as starting material for the cloning of recurrent translocations. In 1998, we established the continuous leukemia cell line MUTZ-5 and its two simultaneous sister cell lines MUTZ-6 and MUTZ-7. The primary specimen was obtained from the...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402212
更新日期:2001-09-01 00:00:00
abstract::We performed cytogenetic and molecular analysis of the BCR-ABL rearrangement by polymerase chain reaction (PCR) in 39 consecutive cases of adult acute lymphoblastic leukemia (ALL). Eleven patients had a Philadelphia (Ph) chromosome. Thirteen patients had a BCR-ABL rearrangement, involving minor breakpoint cluster regi...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Genomic analyses of chronic lymphocytic leukemia (CLL) identified somatic mutations and associations of clonal diversity with adverse outcomes. Clonal evolution likely has therapeutic implications but its dynamic is less well studied. We studied clonal composition and prognostic value of seven recurrently mutated driv...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/s41375-018-0215-9
更新日期:2019-02-01 00:00:00
abstract::Myelofibrosis (MF; primary or post-essential thrombocythemia/polycythemia vera) is incurable clonal myeloproliferative disorder, with no effective treatment. Epigenetic changes play an important role in cancer pathogenesis through transcriptional silencing of critical tumor suppressor genes. We conducted a phase-II st...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2008.91
更新日期:2008-05-01 00:00:00
abstract::During infection with different retroviruses, high levels of unintegrated extrachromosomal DNA accumulate in infected cells. While extrachromosomal linear DNA is the immediate precursor of the integrated provirus, the function, if any, of extrachromosomal circular DNA has been unclear. Several groups have attempted to...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/sj.leu.2400776
更新日期:1997-09-01 00:00:00
abstract::Imatinib is a highly effective therapy for chronic phase-chronic myeloid leukaemia (CP-CML) patients; however, responses to frontline imatinib are variable. The human organic cation transporter 1 (OCT1; SLC22A1) has been reported to be the main influx transporter involved in imatinib uptake into CML cells. Furthermore...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/leu.2015.170
更新日期:2015-10-01 00:00:00
abstract::The purpose of this study was to determine the incidence of and pharmacokinetic parameters associated with the development of transient encephalopathy following the administration of high-dose methotrexate and intrathecal chemotherapy in children with acute lymphoblastic leukemia (ALL). Two hundred and fifty-nine chil...
journal_title:Leukemia
pub_type: 临床试验,杂志文章
doi:10.1038/sj.leu.2401098
更新日期:1998-08-01 00:00:00
abstract::Previously, we identified SETD2 loss-of-function mutations in 22% of MLL-rearranged (MLLr) acute leukemia patients, implicating a mechanism for cooperativity between SETD2 mutations and MLL fusions. However, the detailed mechanism of how SETD2-H3K36me3 downregulation accelerates MLLr leukemia remains unclear. Here, we...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2017.339
更新日期:2018-04-01 00:00:00
abstract::Outcomes for patients with chronic myeloid leukemia (CML) have substantially improved due to advances in drug development and rational treatment intervention strategies. Despite these significant advances there are still unanswered questions on patient management regarding how to more reliably predict treatment failur...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/s41375-019-0512-y
更新日期:2019-08-01 00:00:00
abstract::Serum levels of cytokines and in vitro cytokine production by lymph node mononuclear cells (LNMC) were studied in four patients with angio-immunoblastic lymphadenopathy with dysproteinemia (AILD) or AILD-type T cell lymphoma. An increased level of serum interleukin-6 (IL-6) was detected on initial diagnosis in both of...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::Between August 1978 and September 1982, 642 patients with newly diagnosed acute myelogenous leukemia (AML) were entered onto a Southwest Oncology Group Study which addressed four questions. (i) What is the comparative utility of rubidazone versus adriamycin in remission induction? (ii) What is the role of prophylactic...
journal_title:Leukemia
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:1992-07-01 00:00:00
abstract::Previous studies demonstrated that triazene compounds (TZC) possess antitumor, antimetastatic and immunosuppressive activity, and induce novel antigenic properties in neoplastic cells. Moreover, TZC showed marked antitumor activity in patients with acute myelogenous leukemias (AML). In most cases leukemic blasts with ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::The choice of adequate controls for reverse transcriptase (RT-) PCR analysis has been the focus of a debate pursued in Leukemia over the past 3 years. Twenty-six authors from 15 different centers contributed to the Debate, and the points presented have been carefully evaluated. This survey reviews the issues discussed...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/sj.leu.2402133
更新日期:2001-07-01 00:00:00
abstract::Fas ligand (FasL) is capable of inducing apoptosis of lymphoid cells by cross-linking with its natural receptor, Fas. We aimed to investigate the possible role of the Fas/FasL-mediated apoptosis in the development of human lymphomas. FasL mRNA was detected by reverse transcriptase-polymerase chain reaction in 38 out o...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2400815
更新日期:1997-11-01 00:00:00
abstract::Analysis at the DNA and RNA level revealed a mature genetic marker profile in a case of T type blast crisis of chronic myelocytic leukemia. T cell receptor beta chain gene rearrangement as well as T cell receptor alpha mRNA transcription was demonstrated in blasts of the malignant clone. Corresponding findings were ob...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract::The polymerase chain reaction (PCR) cannot be used to amplify the breakpoint in the chimaeric BCR-ABL gene in CML and acute leukaemias due to the large variation in the sites of breakpoint in the BCR gene (within a 5.8 kb region) and in the ABL gene (within a 150 kb region). The disease state is usually monitored usin...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::The stem cell leukaemia (SCL) gene is a member of the basic helix-loop-helix family of transcription factors and is essential for the development of all haematopoietic lineages. SCL is expressed in pluripotent haematopoietic stem cells and also following commitment to the erythroid, mast and megakaryocytic lineages. T...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401420
更新日期:1999-05-01 00:00:00
abstract::In vitro enzymatic amplification and direct sequencing were used to detect and characterize t(14;18) recombination junctions in peripheral blood and bone marrow mononuclear cell preparations from patients with follicular lymphoma in remission. Samples from 24/44 patients were found to be positive for translocations in...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::I use the hematological, morphological and molecular criteria recently established by the World Health Organization to diagnose essential thrombocytemia. In these patients, major causes of morbidity and mortality are represented by thrombosis and bleeding, whereas progression to myelofibrosis and transformation to acu...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/leu.2011.306
更新日期:2012-05-01 00:00:00
abstract::Multiple myeloma (MM) is a plasma cell tumor marked by clonal evolution and preceded by a premalignant stage, which progresses via molecular pathway deregulation, including MYC activation. This activation relates to translocation or gain of the MYC locus and deregulation of upstream pathways such as IRF4, DIS3/LIN28B/...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/s41375-018-0036-x
更新日期:2018-06-01 00:00:00
abstract::The proto-oncogene c-Jun has been implicated in the control of cell proliferation and differentiation and more recently in the regulation of apoptosis. We have previously reported the involvement of c-Jun in the erythroid differentiation block in murine erythroleukemia (MEL) cells. As reported here, we investigated th...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402350
更新日期:2002-02-01 00:00:00
abstract::The inv(16)(p13q22) chromosomal rearrangement associated with FAB M4Eo acute myeloid leukemia (AML) subtype is characterized by the presence of the CBFbeta/MYH11 fusion transcript that can be used to detect minimal residual disease (MRD). However, qualitative RT-PCR studies of MRD have so far produced conflicting resu...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402478
更新日期:2002-06-01 00:00:00
abstract::Interferon-alpha produces a complete hematologic and cytogenetic remission in approximately 20% of patients with chronic myelogenous leukemia (CML). In this study, we applied fluorescent in situ hybridization (FISH) methodology to examine the possibility that a low level of proliferating Philadelphia-chromosome-positi...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::Of 51 infants with acute leukemia, 13 (25%) had contradictory findings on 11q23/MLL rearrangements that were analyzed by cytogenetic and Southern blot methods: seven had rearranged MLL and normal karyotype, four had rearranged MLL and abnormal karyotype with no 11q23 translocation, and two had germline MLL and 11q23 t...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402900
更新日期:2003-05-01 00:00:00
abstract::Reciprocal rearrangements of the MLL gene are among the most common chromosomal abnormalities in both Acute Lymphoblastic and Myeloid Leukemia. The MLL gene, located on the 11q23 chromosomal band, is involved in more than 40 recurrent translocations. In the present study, we describe the development and validation of ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2403439
更新日期:2004-09-01 00:00:00
abstract::We have investigated the activity of ITF2357, a novel hydroxamate histone deacetylase inhibitor, on multiple myeloma (MM) and acute myelogenous leukemia (AML) cells in vitro and in vivo. ITF2357 induced apoptosis in 8/9 MM and 6/7 AML cell lines, as well as 4/4 MM and 18/20 AML freshly isolated cases, with a mean IC(5...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2404860
更新日期:2007-09-01 00:00:00
abstract::T-cell large granular lymphocytic (LGL) leukemia is a complex diagnosis, requiring persistent clonal expansions of LGLs, and cytopenias. Often the diagnosis is unclear as non-clonal expansions of LGLs commonly occur in reactive conditions. To better understand T-LGL leukemia, we performed a comprehensive clinicopathol...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2011.107
更新日期:2011-09-01 00:00:00
abstract::Monoclonal antibody (mAb) M195 is a mouse IgG2a reactive with a myelomonocytic differentiation antigen found on early myeloid cells and monocytes. The reactivity of M195 with fresh hematopoietic neoplasms in the blood or bone marrow from 227 patients at Memorial Hospital was determined by flow cytometry. M195 was posi...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
abstract::An increased number of circulating CD34+ hematopoietic progenitors with a prominent proliferation of the megakaryocytic (MK) population are the hallmarks of the myeloproliferation in myelofibrosis with myeloid metaplasia (MMM). Analyzing the potential contribution of the stem cell leukemia (SCL) gene in MMM myeloproli...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2403089
更新日期:2003-10-01 00:00:00
abstract::The presence of CD19 in myelomatous plasma cells (MM-PCs) correlates with adverse prognosis in multiple myeloma (MM). Although CD19 expression is upregulated by CD81, this marker has been poorly investigated and its prognostic value in MM remains unknown. We have analyzed CD81 expression by multiparameter flow cytomet...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2012.42
更新日期:2012-08-01 00:00:00