Abstract：
:A total of 57 strains of Salmonella spp. were differentiated by random amplification of polymorphic DNA (RAPD) fingerprinting using three different primers (OPL-03, primer 1, and primer A); by Enterobacterial Repetitive Intergenic Consensus (ERIC) fingerprinting; by ribotyping-PCR; and by Single Strand Conformation Polymorphism (SSCP). From the 57 strains, RAPD fingerprinting with primers OPL-03, 1, and A produced 42, 51, and 54 fingerprint patterns respectively. ERIC fingerprinting produced 50 patterns; ribotyping-PCR produced four patterns, and SSCP produced 11 patterns. Combinations of two different typing methods generally increased the discrimination of Salmonella strains. A combination of two different RAPDs or a combination of RAPD and ERIC was better than the other combinations. Discrimination using a combination of RAPD (primer 1 or primer A) and ERIC, which could differentiate all 57 Salmonella strains, was better than the combination of two RAPDs. This study indicated that the use of a combination of RAPD (primer 1 or primer A) and ERIC should be useful for the differentiation of field-isolated Salmonella strains and epidemiological studies.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Lim H,Lee KH,Hong CH,Bahk GJ,Choi WSdoi
10.1016/j.ijfoodmicro.2005.03.019subject
Has Abstractpub_date
2005-12-15 00:00:00pages
411-8issue
3eissn
0168-1605issn
1879-3460pii
S0168-1605(05)00343-0journal_volume
105pub_type
杂志文章abstract：:In this work, psychrotrophs known to cause food spoilage were isolated from commercial food products. Further, temperature sensitivities and volatile organic compounds of the representative strains were characterized to evaluate the population heterogeneity. A total of 490 isolates belonging to 38 genera of 20 familie...
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更新日期：2019-02-02 00:00:00
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journal_title：International journal of food microbiology
pub_type： 杂志文章
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更新日期：2005-11-25 00:00:00
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更新日期：2018-04-20 00:00:00
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journal_title：International journal of food microbiology
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更新日期：2008-02-10 00:00:00
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pub_type： 杂志文章
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更新日期：2021-01-12 00:00:00
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journal_title：International journal of food microbiology
pub_type： 杂志文章
doi：10.1016/j.ijfoodmicro.2016.12.019
更新日期：2017-03-06 00:00:00
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journal_title：International journal of food microbiology
pub_type： 杂志文章
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journal_title：International journal of food microbiology
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更新日期：2018-04-02 00:00:00
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journal_title：International journal of food microbiology
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journal_title：International journal of food microbiology
pub_type： 杂志文章
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更新日期：2017-01-02 00:00:00
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pub_type： 杂志文章
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journal_title：International journal of food microbiology
pub_type： 杂志文章
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journal_title：International journal of food microbiology
pub_type： 杂志文章,评审
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更新日期：2008-08-15 00:00:00
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journal_title：International journal of food microbiology
pub_type： 杂志文章
doi：10.1016/j.ijfoodmicro.2018.07.001
更新日期：2018-11-02 00:00:00
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journal_title：International journal of food microbiology
pub_type： 杂志文章
doi：10.1016/s0168-1605(98)00180-9
更新日期：1999-03-01 00:00:00
