Abstract：
:Sixty vacuum-packed beef samples retailed in Germany were investigated for the occurrence of cold-tolerant Clostridium spp. After a storage period at 4 °C for eight weeks, meat juice from all samples was processed for culturing, DNA extraction and SYBR green qPCR for Clostridium species. After that, a previously developed multiplex qPCR, sequence analysis of the 16S rRNA gene, and MALDI-TOF MS were applied in order to identify Clostridium spp. found in samples. Subsequently, 23 samples were found positive for C. frigoriphilum (n = 19), C. estertheticum (n = 2), C. tagluense (n = 1) and C. lacusfryxellense/C. frigoris (n = 1). By using a new multiplex qPCR and a new RFLP method developed in this study, a further 15 meat juice samples were revealed to be contaminated with C. algidicarnis. With some samples being co-contaminated with two different species, 53% (n = 32) of all investigated vacuum-packed beef samples were found to be positive for cold-tolerant clostridia. This is the first report of detection and identification of C. algidicarnis in meat samples in Germany and Central Europe.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Mang S,Schwaiger K,Lindner R,Gareis M,Dorn-In Sdoi
10.1016/j.ijfoodmicro.2021.109053subject
Has Abstractpub_date
2021-01-12 00:00:00pages
109053eissn
0168-1605issn
1879-3460pii
S0168-1605(21)00012-Xjournal_volume
340pub_type
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