Detection of Clostridium perfringens type A enterotoxin in faecal and food samples using immunomagnetic separation (IMS)-ELISA.

Abstract:

:A simple, rapid and sensitive immunoassay, based on immunomagnetic particles (Dynabeads M-280) was developed for detection and quantitation of Clostridium perfringens type A enterotoxin from faecal and food extracts. The assay had a detection limit of 2.5 ng/ml enterotoxin in homogenates of faeces and inoculated meat extracts. The specificity was confirmed by both crossed immunoelectrophoresis and Western immunoblotting techniques, using a purified enterotoxin as standard.

journal_name

Int J Food Microbiol

authors

Cudjoe KS,Thorsen LI,Sørensen T,Reseland J,Olsvik O,Granum PE

doi

10.1016/0168-1605(91)90145-f

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

313-21

issue

4

eissn

0168-1605

issn

1879-3460

pii

0168-1605(91)90145-F

journal_volume

12

pub_type

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