Abstract:
:Replication defective vectors derived from simple retroviruses or the more complex genomes of lentiviruses continue to offer the advantages of long-term expression, cell and tissue specific tropism, and large packaging capacity for the delivery of therapeutic genes. The occurrence of adverse events caused by insertional mutagenesis in three patients in a gene therapy trial for X-linked SCID emphasizes the potential for problems in translating this approach to the clinic. Several genome-wide studies of retroviral integration are now providing novel insights into the integration site preferences of different vector classes. We review recent developments in vector design, integration, biosafety, and production.
journal_name
Gene Therjournal_title
Gene therapyauthors
Sinn PL,Sauter SL,McCray PB Jrdoi
10.1038/sj.gt.3302570subject
Has Abstractpub_date
2005-07-01 00:00:00pages
1089-98issue
14eissn
0969-7128issn
1476-5462pii
3302570journal_volume
12pub_type
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