Abstract：
:An invasive Escherichia coli expressing the inv gene from Yersinia pseudotuberculosis was used as a vector for protein delivery to mammalian epithelial cells. Upon incubation with beta1-integrin-expressing mammalian cells, the bacteria are internalized, allowing bacteria-encoded proteins to function from within the mammalian cell. These bacteria are eventually processed in the host phagosome where they are destroyed. Expression of listeriolysin O from Listeria monocytogenes in the bacterium and its subsequent release into the phagosome triggers the breakdown of the membrane, allowing the release of the bacterial content into the cytosol of host cells. Using this vector, we demonstrate delivery of a gene and intact, functional proteins into mammalian cells in which beta1-integrin is expressed and accessible. At a ratio of bacteria/mammalian cells compatible with the survival of the mammalian cells, protein delivery can be observed in the entire cell population in vitro, while gene transfer is far less efficient. Protein delivery can also be achieved in vivo in mouse tumour models and can be detected at least 96 h after inoculation. Functional, natural E. coli proteins are delivered in the process and can provide therapeutic benefit in vivo, when associated with prodrugs. This therapeutic effect is associated with infiltration of neutrophils, eosinophils, macrophages and to a lesser extent dendritic cells in the tumour mass.
journal_name
Gene Therjournal_title
Gene therapyauthors
Critchley RJ,Jezzard S,Radford KJ,Goussard S,Lemoine NR,Grillot-Courvalin C,Vassaux Gdoi
10.1038/sj.gt.3302281keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
1224-33issue
15eissn
0969-7128issn
1476-5462pii
3302281journal_volume
11pub_type
杂志文章相关文献
GENE THERAPY文献大全abstract：:Newcastle disease virus (NDV) is an oncolytic paramyxovirus with a nonsegmented single-stranded RNA genome. In this report, a recombinant oncolytic NDV was passaged in human tumor xenografts and reisolated and characterized after two rounds of bioselection. Several isolates could be recovered that differed from the pa...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2012.13
更新日期：2013-01-01 00:00:00
abstract：:Gene transfer technology has opened novel treatment avenues toward the treatment of damaged musculoskeletal tissues, and may be particularly beneficial to articular cartilage. There is no natural repair mechanism to heal damaged or diseased cartilage. Existing pharmacologic, surgical and cell based treatments may offe...
journal_title：Gene therapy
pub_type： 杂志文章,评审
doi：10.1038/sj.gt.3302201
更新日期：2004-02-01 00:00:00
abstract：:Mesenchymal stem cells (MSC) are a group of clonogenic cells present among the bone marrow stroma and capable of multilineage differentiation into mesoderm-type cells such as osteoblasts, adipocytes and chondrocytes. Due to their ease of isolation and their differentiation potential, MSC are being introduced into clin...
journal_title：Gene therapy
pub_type： 杂志文章,评审
doi：10.1038/sj.gt.3303067
更新日期：2008-01-01 00:00:00
abstract：:We have previously shown that recombinant Sendai virus (SeV) vector, derived from murine parainfluenza virus, is one of the most efficient vectors for airway gene transfer. We have also shown that SeV-mediated transfection on second administration, although reduced by 60% when compared with levels achieved after a sin...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2010.131
更新日期：2011-02-01 00:00:00
abstract：:Glucagon-like peptide 1 (GLP-1) and its analogue exendin-4 (Ex4) have displayed potent glucose homeostasis-modulating characteristics in type 2 diabetes (T2D). However, there are few reports of effectiveness in type 1 diabetes (T1D) therapy, where there is massive loss of beta cells. We previously described a novel GL...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302944
更新日期：2007-06-01 00:00:00
abstract：:Small interfering RNAs (siRNAs) are emerging as promising therapeutic tools. However, the widespread clinical application of such molecules as modulators of gene expression is still dependent on several aspects that limit their bioavailability. One of the most promising strategies to overcome the barriers faced by gen...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2011.27
更新日期：2011-07-01 00:00:00
abstract：:The role of gap junctional intercellular communication (GJIC) in bystander killing with herpes simplex virus thymidine kinase (HSV-TK) and ganciclovir (GCV) was evaluated in U251 cells expressing a dominant-negative connexin 43 cDNA (DN14), and in HeLa cells, reportedly devoid of connexin protein. These cell lines bot...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302487
更新日期：2005-07-01 00:00:00
abstract：:In an earlier study exploring the potential of gene transfer to repair myocardial conduction defects, we observed that myotubes, generated by forced expression of MyoD, exhibit reduced excitability when also modified to express connexin43 (Cx43). We hypothesized that this effect was caused by gap junction-mediated cou...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302813
更新日期：2006-11-01 00:00:00
abstract：:Lentiviral vectors pseudotyped with the baculovirus envelope protein GP64 transduce primary cultures of human airway epithelia (HAE) at their apical surface. Our goal in this study was to harness a directed evolution approach to develop a novel envelope glycoprotein with increased transduction properties for HAE. Usin...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2017.78
更新日期：2017-10-01 00:00:00
abstract：:We introduce a lung inflation-fixation protocol to examine the distribution and gene transfer efficiency of fluorescently tagged lipoplexes using fluorescence confocal microscopy within thick lung tissue sections. Using this technique, we tested the hypothesis that factors related to lipoplex distribution were the pre...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301461
更新日期：2001-06-01 00:00:00
abstract：:With the long-term goal of developing a gene-based treatment for osteoarthritis (OA), we performed studies to evaluate the equine joint as a model for adeno-associated virus (AAV)-mediated gene transfer to large, weight-bearing human joints. A self-complementary AAV2 vector containing the coding regions for human inte...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2012.81
更新日期：2013-06-01 00:00:00
abstract：:Cell transplantation holds promise as a potential treatment for cardiac dysfunction. Our group has isolated populations of murine skeletal muscle-derived stem cells (MDSCs) that exhibit stem cell-like properties. Here, we investigated the fate of MDSCs after transplantation into the hearts of dystrophin-deficient mdx ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302521
更新日期：2005-08-01 00:00:00
abstract：:Solid tumors meet their demands for nascent blood vessels and increased glycolysis, to combat hypoxia, by activating multiple genes involved in angiogenesis and glucose metabolism. Hypoxia inducible factor-1 (HIF-1) is a constitutively expressed basic helix-loop-helix transcription factor, formed by the assembly of HI...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301388
更新日期：2001-04-01 00:00:00
abstract：:Retroviral vectors are being used increasingly in clinical gene therapy protocols but low transduction frequencies are presenting a significant obstacle to progress. In this paper we report a simple method to enhance the efficiency of ex vivo retroviral gene transfer. Calcium chloride is added to the vector stock and ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Mobilized blood CD34+ cells from cancer patients were ex vivo infected by a recombinant adenovirus vector carrying an alkaline phosphatase gene, whose expression is evaluable by flow cytometry. A mean of 40% CD34+ cells were infected by the vector, with high levels of expression of the transgene. Among attempts to imp...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300620
更新日期：1998-04-01 00:00:00
abstract：:Transplantation of allogeneic pancreatic islets is an effective approach to treat type 1 diabetes. To bypass the need for systemic administration of immunosuppression drugs following transplantation, approaches to genetically modify allogeneic islets to express anti-inflammatory, immunosuppressive, or antiapoptotic pr...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302530
更新日期：2005-09-01 00:00:00
abstract：:Various methods for determining the expression of the beta-galactosidase (beta-gal) gene after retroviral transduction were compared as a means to assess retroviral titre. To allow better comparison, different retroviral vectors were constructed carrying two mutants of the green fluorescent protein and assessed as sen...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300519
更新日期：1997-11-01 00:00:00
abstract：:Cationic lipid-DNA complexes (lipoplexes) have been widely used as gene transfer vectors which avoid the adverse immunogenicity and potential for viraemia of viral vectors. With the long-term aim of gene transfer into skeletal muscle in vivo, we describe a direct in vitro comparison of two commercially available catio...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300604
更新日期：1998-04-01 00:00:00
abstract：:In vitro culture systems of human myogenic cells contribute greatly to elucidation of the molecular mechanisms underlying terminal myogenic differentiation and symptoms of neuromuscular diseases. However, human myogenic cells have limited ability to proliferate in culture. We have established an improved immortalizati...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2011.44
更新日期：2011-09-01 00:00:00
abstract：:We have developed a novel immunostimulatory molecule against tumor cells, composed of an anti-FcgammaRIII (CD16) scFv fused to the platelet-derived growth factor receptor (PDGFR) transmembrane region. This fusion molecule was stably expressed on the tumor cell surface and retained the ability of the parental antibody ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301575
更新日期：2001-11-01 00:00:00
abstract：:Intramuscular (i.m.) injection of a plasmid encoding human carcinoembryonic antigen (CEA) elicited immunity against transplanted syngeneic (C57BL/6) CEA-positive Lewis lung carcinoma (CEA/LLC) cells, but tumors still appeared in all mice. In wild-type mice, coinjection of an IL-12 plasmid markedly enhanced anti-CEA hu...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3301274
更新日期：2000-09-01 00:00:00
abstract：:RNA interference (RNAi) was reported to block hepatitis B virus (HBV) gene expression and replication in vitro and in vivo. However, it remains a technical challenge for RNAi-based therapy to achieve long-term and complete inhibition effects in chronic HBV infection, which presumably requires more extensive and unifor...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302846
更新日期：2007-01-01 00:00:00
abstract：:HaCaT cells, a spontaneously immortalised, nontumorigenic keratinocyte line, were used as a more amenable model than primary keratinocytes for ex vivo-mediated gene transfer. These cells were transduced with retroviral vectors containing the factor IX cDNA under the control of a cytomegaloviral (CMV) promoter/enhancer...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300591
更新日期：1998-03-01 00:00:00
abstract：:Overexpression of vascular endothelial growth factor (VEGF) and its cognate receptor KDR has been linked to a more aggressive phenotype of human prostate carcinomas. The importance of signal transduction through the VEGF receptor 2 is illustrated by use of soluble KDR, which binds to VEGF and sequesters this ligand be...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302432
更新日期：2005-03-01 00:00:00
abstract：:We have compared the efficacy of daily injection of recombinant leptin protein (rh-leptin) with adenovirus-mediated delivery of the murine or human leptin gene (Ad-leptin) for treatment of obesity in the obese (ob/ob) mouse model. We demonstrate an improved correction profile for obesity and associated surrogate marke...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300565
更新日期：1998-01-01 00:00:00
abstract：:Current tools for the inhibition of microRNA (miR) function are limited to modified antisense oligonucleotides, sponges and decoy RNA molecules and none have been used to understand miR function during development. CRISPR/Cas-mediated deletion of miR sequences within the genome requires multiple chromosomal deletions ...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2016.22
更新日期：2016-06-01 00:00:00
abstract：:Studies reviewed show that lentiviral gene therapy directed either at inhibiting the synthesis of brain acetaldehyde generated from ethanol or at degrading brain acetaldehyde fully prevent ethanol intake by rats bred for their high alcohol preference. However, after animals have chronically consumed alcohol, the above...
journal_title：Gene therapy
pub_type： 杂志文章,评审
doi：10.1038/s41434-019-0064-9
更新日期：2019-11-01 00:00:00
abstract：:The current therapies to treat hepatitis B virus (HBV) infection are limited. Recently, clustered regularly interspaced short palindromic repeat (CRISPR) systems, originally identified in bacteria and archaea, have been found to consist of an RNA-based adaptive immune system that degrades complimentary sequences of in...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/gt.2015.2
更新日期：2015-05-01 00:00:00
abstract：:This study examines the clinical relevance of tissue engineering integrating gene therapy and polymer science to bone regeneration. Bilateral maxillary defects (3 x 1.2 cm(2)) in 20 miniature swine were bridged with a bioresorbable internal splint. Constructs were created using ex vivo adenovirus bone morphogenetic pr...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3302106
更新日期：2003-11-01 00:00:00
abstract：:To elucidate further the potential of a Semliki Forest virus (SFV) vector in vivo for gene therapy, we constructed a vector, SFV-IL12, to transfer murine IL-12 genes into tumors. A single intratumoral injection of established B16 murine melanoma with SFV-IL12 resulted in a significant inhibition of tumor growth, while...
journal_title：Gene therapy
pub_type： 杂志文章
doi：10.1038/sj.gt.3300841
更新日期：1999-04-01 00:00:00
