Abstract:
:Newcastle disease virus (NDV) is an oncolytic paramyxovirus with a nonsegmented single-stranded RNA genome. In this report, a recombinant oncolytic NDV was passaged in human tumor xenografts and reisolated and characterized after two rounds of bioselection. Several isolates could be recovered that differed from the parental virus with respect to virus spread in tumor cells and the ability to form syncytia in human tumor cells. Three isolates were identified that demonstrated superior oncolytic potency compared with the parental virus as measured by increased oncolytic potency in confluent tumor cell monolayers, in tumor cell spheroids and in a mouse xenograft tumor model. The surface proteins F and HN were sequence analyzed and characterized for fusogenicity. The present study demonstrates that in vivo NDV bioselection can enable the isolation of novel, oncolytic NDV and thus represents a powerful methodology for the development of highly potent oncolytic viruses.
journal_name
Gene Therjournal_title
Gene therapyauthors
Beier R,Hermiston T,Mumberg Ddoi
10.1038/gt.2012.13subject
Has Abstractpub_date
2013-01-01 00:00:00pages
102-11issue
1eissn
0969-7128issn
1476-5462pii
gt201213journal_volume
20pub_type
杂志文章相关文献
GENE THERAPY文献大全abstract::Retroviral vectors have become the primary tool for gene delivery into hematopoietic cells, including T lymphocytes. Lentiviral vectors offer an advantage over Moloney murine leukemia virus (MuLV) vectors because of their ability to translocate across an intact nuclear membrane and integrate into the genome of nonprol...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3301378
更新日期:2001-02-01 00:00:00
abstract::In 2012, we reported that 5 out of 11 subjects in a clinical trial (NCT00372320) administering AdhAQP1 to radiation-damaged parotid glands showed increased saliva flow rates and decreased symptoms over the initial 42 days. AdhAQP1 is a first-generation, E1-deleted, replication-defective, serotype 5 adenoviral vector e...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2015.55
更新日期:2015-09-01 00:00:00
abstract::Because periodontal ligament (PDL) cells are reported to contain progenitor or stem cell populations, they are considered a beneficial cell source for clinical periodontal regeneration. Both bone morphogenetic protein 4 (BMP4) and human telomerase reverse transcriptase (hTERT) have essential roles in the modulation of...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2010.158
更新日期:2011-05-01 00:00:00
abstract::Alveolar bone loss associated with periodontal diseases is the result of osteoclastogenesis induced by bacterial pathogens. The mitogen-activated protein kinase (MAPK) phosphatase 1 (MKP-1) is a critical negative regulator of immune response as a key phosphatase capable of dephosphorylating activated MAPKs. In this st...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2010.139
更新日期:2011-04-01 00:00:00
abstract::Signal transducer and activator of transcription 3 (STAT3) is constitutively activated in diverse cancers, which contributes to the proliferation and survival of cancer cells by upregulating apoptosis inhibitors and cell cycle regulators. Suppressor of cytokine signaling 1 (SOCS1) is an important negative regulator of...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2012.4
更新日期:2013-01-01 00:00:00
abstract::The canine is the most important large animal model for testing novel hemophilia A (HA) treatment. It is often necessary to use canine factor VIII (cFIII) gene or protein for the evaluation of HA treatment in the canine model. However, different biological properties between cFVIII and human FVIII (hFVIII) indicated t...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2016.34
更新日期:2016-07-01 00:00:00
abstract::The development of lentiviral vectors that use Rev-independent mechanisms of nuclear export for their genomic RNA could facilitate the construction of novel anti-HIV vectors. We have improved the titers of Rev-independent lentiviral vectors having the SRV-1 CTE by mutating the major splice donor and acceptor sites pre...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3301262
更新日期:2000-08-01 00:00:00
abstract::In the last two decades, remarkable advances have been made in the development of technologies used to engineer new aptamers and ribozymes. This has encouraged interest among researchers who seek to create new types of gene-control systems that can be made to respond specifically to small-molecule signals. Validation ...
journal_title:Gene therapy
pub_type: 杂志文章,评审
doi:10.1038/gt.2009.81
更新日期:2009-10-01 00:00:00
abstract::Gene therapy is thought to be a promising method for the treatment of various diseases. One gene therapy strategy involves the manipulations on a process of formation of new vessels, commonly defined as angiogenesis. Angiogenic and antiangiogenic gene therapy is a new therapeutic approach to the treatment of cardiovas...
journal_title:Gene therapy
pub_type: 杂志文章,评审
doi:10.1038/sj.gt.3302621
更新日期:2005-10-01 00:00:00
abstract::Confinement of transgene expression to target cells is highly desirable in gene therapy. Current strategies of transcriptional targeting to tumors usually rely on tissue-specific promoters to control gene expression. However, such promoters generally have much lower activity than the constitutive viral promoters. We h...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3302029
更新日期:2003-08-01 00:00:00
abstract::Virotherapy is currently being developed for many different types of viruses including replication-competent murine leukaemia virus (MLV) as a novel tool in cancer therapy. However, there is the risk of insertional mutagenesis associated with this virus, making careful preclinical studies necessary before its first ap...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2008.92
更新日期:2008-11-01 00:00:00
abstract::Replicating viruses for cancer gene therapy have beneficial antitumor effects, however, in the setting of an enzyme/prodrug system, the interactions between these viruses and the activated agents are complex. A replicating vaccinia virus expressing the cytosine deaminase gene (VVCD), which converts the prodrug 5-FC in...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3301237
更新日期:2000-07-01 00:00:00
abstract::Mobilized blood CD34+ cells from cancer patients were ex vivo infected by a recombinant adenovirus vector carrying an alkaline phosphatase gene, whose expression is evaluable by flow cytometry. A mean of 40% CD34+ cells were infected by the vector, with high levels of expression of the transgene. Among attempts to imp...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3300620
更新日期:1998-04-01 00:00:00
abstract::The failure of pharmacological approaches to cure infection with the human immunodeficiency virus (HIV) has renewed the interest in gene-based therapies. Among the various strategies that are currently explored, the blockade of HIV entry into susceptible T cells and macrophages promises to be the most powerful interve...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3302755
更新日期:2006-07-01 00:00:00
abstract::Urea cycle defects presenting in the neonatal period with hyperammonaemia are associated with high morbidity and mortality, and necessitate liver transplantation for long-term management. Gene therapy is therefore an attractive possibility, with vectors based on adeno-associated virus (rAAV) currently showing exciting...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2013.51
更新日期:2013-12-01 00:00:00
abstract::Partial resistance of primary mouse hepatocytes to lentiviral (LV) vector transduction poses a challenge for ex vivo gene therapy protocols in models of monogenetic liver disease. We thus sought to optimize ex vivo LV gene transfer while preserving the hepatocyte integrity for subsequent transplantation into recipient...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2011.117
更新日期:2012-04-01 00:00:00
abstract::The ability to restrict gene delivery and expression to particular cell types is of paramount importance for many types of gene therapy, especially in the lung. The alveolar epithelial type I (ATI) cell, in particular, is an attractive cell type to target, as it comprises 95% of the internal surface area of the lung. ...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2016.52
更新日期:2016-10-01 00:00:00
abstract::Selection of targeted vectors from virus display peptide libraries is a versatile and efficient approach to improve vector specificity and efficiency. This strategy has been used to target various cell types in vitro. Here, we report the screening of an adeno-associated virus type 2 (AAV2) display peptide library in v...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2010.44
更新日期:2010-08-01 00:00:00
abstract::The existence of cells with stem cell-like abilities derived from various tissues can now be extended to include the skeletal muscle compartment. Although researchers have focused on the utilization of these cells with regard to their myogenic capacity, initially exploring more efficient cellular therapy treatments fo...
journal_title:Gene therapy
pub_type: 杂志文章,评审
doi:10.1038/sj.gt.3301719
更新日期:2002-05-01 00:00:00
abstract::There is currently an urgent need to develop efficient gene-delivery systems for the lung that are free of inflammatory effects. The LID vector is a synthetic gene delivery system, comprised of lipofectin (L), an integrin-targeting peptide (I) and DNA (D) that has previously been shown to have high transfection effici...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3301963
更新日期:2003-06-01 00:00:00
abstract::Hydrodynamic gene delivery to the liver is an attractive approach for clinical liver gene therapy, but critical aspects of technique remain uncertain. There has not been to date any report of high levels of hydrodynamic gene delivery to the liver, except in rodents. Regional hydrodynamic delivery to individual lobes/s...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2009.167
更新日期:2010-04-01 00:00:00
abstract::Until recently, adeno-associated virus 9 (AAV9) was considered the AAV serotype most effective in crossing the blood-brain barrier (BBB) and transducing cells of the central nervous system (CNS), following systemic injection. However, a newly engineered capsid, AAV-PHP.B, is reported to cross the BBB at even higher ef...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/s41434-018-0005-z
更新日期:2018-04-01 00:00:00
abstract::Gene targeting by single-stranded oligodeoxyribonucleotides (ssODNs) is a promising technique for introducing site-specific sequence alterations without affecting the genomic organization of the target locus. Here, we discuss the significant progress that has been made over the last 5 years in unraveling the mechanism...
journal_title:Gene therapy
pub_type: 杂志文章,评审
doi:10.1038/gt.2010.161
更新日期:2011-03-01 00:00:00
abstract::To achieve high transgene expression in the liver, we have compared the reporter gene expression among various murine retroviral long terminal repeats (LTRs) or leader sequences in vitro. Transient reporter gene expression assays revealed the highest gene expression by the polycythemic strain of spleen focus-forming v...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3301655
更新日期:2002-02-01 00:00:00
abstract::Isolated limb perfusion (ILP) is a limb salvage surgical modality used to deliver chemotherapy and biologic agents to locally advanced and recurrent extremity soft tissue sarcoma (STS), and may be readily tailored for delivery of gene therapy. We set out to test the feasibility of delivering AdFLAGp53 (replication inc...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3302911
更新日期:2007-04-01 00:00:00
abstract::This study was designed to determine whether Coxsackie adenovirus receptor (CAR) and alpha nu beta3/alpha nu beta5 integrin co-receptors are involved in adenovirus gene transfer in the rat cochlea. We find that CAR and integrin co-receptors are expressed in every cell subtype transduced by the adenoviral vector Ad5 De...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3302826
更新日期:2007-01-01 00:00:00
abstract::In muscle, mutant genes can be targeted and corrected directly by intramuscular (i.m.) injection of corrective DNA, or by ex vivo delivery of DNA to myogenic cells, followed by cell transplantation. Short fragment homologous replacement (SFHR) has been used to repair the exon 23 nonsense transition at the Xp21.1 dys l...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3301737
更新日期:2002-06-01 00:00:00
abstract::Cellular immunity against cancer can be achieved with viral vector- and DNA-based immunizations. In preclinical studies, cancer vaccines are very potent, but in clinical trials these potencies are not achieved yet. Thus, a rational approach to improve cancer vaccines is warranted. We previously demonstrated that the r...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/gt.2015.24
更新日期:2015-07-01 00:00:00
abstract::The potential for gene therapy to be an effective treatment for cystic fibrosis has been hampered by the limited gene transfer efficiency of current vectors. We have shown that recombinant Sendai virus (SeV) is highly efficient in mediating gene transfer to differentiated airway epithelial cells, because of its capaci...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3302991
更新日期:2007-10-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infection ultimately leads to the destruction of the CD4+ lymphocyte subset and the onset of AIDS. In recent years, several gene therapy procedures making use of retroviral vectors that selectively target HIV susceptible cells have been proposed in order to interfere with HIV product...
journal_title:Gene therapy
pub_type: 杂志文章
doi:10.1038/sj.gt.3300646
更新日期:1998-05-01 00:00:00