Abstract:
:In a European BIOMED-2 collaborative study, multiplex PCR assays have successfully been developed and standardized for the detection of clonally rearranged immunoglobulin (Ig) and T-cell receptor (TCR) genes and the chromosome aberrations t(11;14) and t(14;18). This has resulted in 107 different primers in only 18 multiplex PCR tubes: three VH-JH, two DH-JH, two Ig kappa (IGK), one Ig lambda (IGL), three TCR beta (TCRB), two TCR gamma (TCRG), one TCR delta (TCRD), three BCL1-Ig heavy chain (IGH), and one BCL2-IGH. The PCR products of Ig/TCR genes can be analyzed for clonality assessment by heteroduplex analysis or GeneScanning. The detection rate of clonal rearrangements using the BIOMED-2 primer sets is unprecedentedly high. This is mainly based on the complementarity of the various BIOMED-2 tubes. In particular, combined application of IGH (VH-JH and DH-JH) and IGK tubes can detect virtually all clonal B-cell proliferations, even in B-cell malignancies with high levels of somatic mutations. The contribution of IGL gene rearrangements seems limited. Combined usage of the TCRB and TCRG tubes detects virtually all clonal T-cell populations, whereas the TCRD tube has added value in case of TCRgammadelta(+) T-cell proliferations. The BIOMED-2 multiplex tubes can now be used for diagnostic clonality studies as well as for the identification of PCR targets suitable for the detection of minimal residual disease.
journal_name
Leukemiajournal_title
Leukemiaauthors
van Dongen JJ,Langerak AW,Brüggemann M,Evans PA,Hummel M,Lavender FL,Delabesse E,Davi F,Schuuring E,García-Sanz R,van Krieken JH,Droese J,González D,Bastard C,White HE,Spaargaren M,González M,Parreira A,Smith JL,Mordoi
10.1038/sj.leu.2403202subject
Has Abstractpub_date
2003-12-01 00:00:00pages
2257-317issue
12eissn
0887-6924issn
1476-5551pii
2403202journal_volume
17pub_type
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